PMID- 35737739
OWN - NLM
STAT- MEDLINE
DCOM- 20230126
LR  - 20230701
IS  - 1554-8635 (Electronic)
IS  - 1554-8627 (Print)
IS  - 1554-8627 (Linking)
VI  - 19
IP  - 2
DP  - 2023 Feb
TI  - Modulation of Atg genes expression in aged rat liver, brain, and kidney by 
      caloric restriction analyzed via single-nucleus/cell RNA sequencing.
PG  - 706-715
LID - 10.1080/15548627.2022.2091903 [doi]
AB  - Dysregulation of macroautophagy/autophagy has been closely implicated in aging. 
      Caloric restriction (CR) is an effective intervention of aging partially via 
      activation of autophagy. Recently, a high-throughput single-cell RNA-seq 
      technique has been employed to detect the comprehensive transcriptomes of 
      individual cells. However, the transcriptional networks of ATG (autophagy 
      related) genes in the aging process and the modulation of ATG genes expression by 
      CR at the single-cell level have not been elucidated. Here, by performing data 
      analysis of single nucleus/cells RNA sequencing in rats undergoing aging and the 
      modulation by CR, we demonstrate that the transcription patterns of Atg genes in 
      different cell types of rat liver, brain, and kidney are highly heterogeneous. 
      Importantly, CR reversed aging-induced changes of multiple Atg genes across 
      different cell types in the brain, liver, and kidney. In summary, our results, 
      for the first time, provide comprehensive information on Atg gene expression in 
      specific cell types of different organs in a mammal during aging and give novel 
      insight into the protective role of autophagy and CR in aging at the single-cell 
      resolution.Abbreviations: ATG genes: autophagy-related genes; Atg5: autophagy 
      related 5; Atg7: autophagy related 7; CR: caloric restriction; DEATG: 
      differentially expressed autophagy-related; NAFLD: nonalcoholic fatty liver 
      disease; ScRNA-seq: single-cell RNA sequencing.
FAU - Yang, Chuanbin
AU  - Yang C
AUID- ORCID: 0000-0001-8288-4038
AD  - Department of Geriatrics, Shenzhen People's Hospital (The Second Clinical Medical 
      College of Jinan University; the First Affiliated Hospital of Southern University 
      of Science and Technology), Shenzhen, Guangdong, China.
FAU - Xia, Siyu
AU  - Xia S
AD  - Department of Geriatrics, Shenzhen People's Hospital (The Second Clinical Medical 
      College of Jinan University; the First Affiliated Hospital of Southern University 
      of Science and Technology), Shenzhen, Guangdong, China.
AD  - Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan 
      University, Guangzhou, Guangdong, China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Geriatrics, Shenzhen People's Hospital (The Second Clinical Medical 
      College of Jinan University; the First Affiliated Hospital of Southern University 
      of Science and Technology), Shenzhen, Guangdong, China.
AD  - Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan 
      University, Guangzhou, Guangdong, China.
FAU - Shen, Han-Ming
AU  - Shen HM
AUID- ORCID: 0000-0001-7369-5227
AD  - Faculty of Health Sciences, University of Macau, Taipa, Macau, China.
FAU - Wang, Jigang
AU  - Wang J
AUID- ORCID: 0000-0002-0575-0105
AD  - Department of Geriatrics, Shenzhen People's Hospital (The Second Clinical Medical 
      College of Jinan University; the First Affiliated Hospital of Southern University 
      of Science and Technology), Shenzhen, Guangdong, China.
AD  - Artemisinin Research Center, Institute of Chinese Materia Medica, China Academy 
      of Chinese Medical Sciences, Beijing, Beijing, China China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220623
PL  - United States
TA  - Autophagy
JT  - Autophagy
JID - 101265188
SB  - IM
MH  - Animals
MH  - Rats
MH  - Aging/genetics/metabolism
MH  - Autophagy/physiology
MH  - Brain/metabolism
MH  - *Caloric Restriction
MH  - Kidney/metabolism
MH  - Mammals/genetics
MH  - *Non-alcoholic Fatty Liver Disease
MH  - Sequence Analysis, RNA
MH  - Cell Nucleus/genetics/metabolism
PMC - PMC9851201
OTO - NOTNLM
OT  - ATGs
OT  - Aging
OT  - autophagy
OT  - brain
OT  - caloric restriction (CR)
OT  - kidney
OT  - liver
OT  - longevity
OT  - single-cell RNA sequencing
COIS- No potential conflict of interest to declare by the authors.
EDAT- 2022/06/24 06:00
MHDA- 2023/01/20 06:00
PMCR- 2023/06/23
CRDT- 2022/06/23 13:54
PHST- 2022/06/24 06:00 [pubmed]
PHST- 2023/01/20 06:00 [medline]
PHST- 2022/06/23 13:54 [entrez]
PHST- 2023/06/23 00:00 [pmc-release]
AID - 2091903 [pii]
AID - 10.1080/15548627.2022.2091903 [doi]
PST - ppublish
SO  - Autophagy. 2023 Feb;19(2):706-715. doi: 10.1080/15548627.2022.2091903. Epub 2022 
      Jun 23.