PMID- 35739192
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2059-0105 (Electronic)
IS  - 2059-0105 (Linking)
VI  - 7
IP  - 1
DP  - 2022 Jun 23
TI  - Engineering the hydroxyl content on aluminum oxyhydroxide nanorod for elucidating 
      the antigen adsorption behavior.
PG  - 62
LID - 10.1038/s41541-022-00495-9 [doi]
LID - 62
AB  - The interaction between the aluminum salt-based adjuvants and the antigen in the 
      vaccine formulation is one of the determining factors affecting the 
      immuno-potentiation effect of vaccines. However, it is not clear how the 
      intrinsic properties of the adjuvants could affect this interaction, which limits 
      to benefit the improvement of existing adjuvants and further formulation of new 
      vaccines. Here, we engineered aluminum oxyhydroxide (AlOOH) nanorods and used a 
      variety of antigens including hepatitis B surface antigen (HBsAg), SARS-CoV-2 
      spike protein receptor-binding domain (RBD), bovine serum albumin (BSA) and 
      ovalbumin (OVA) to identify the key physicochemical properties of adjuvant that 
      determine the antigen adsorption at the nano-bio interface between selected 
      antigen and AlOOH nanorod adjuvant. By using various physicochemical and 
      biophysical characterization methods, it was demonstrated that the surface 
      hydroxyl contents of AlOOH nanorods affected the adsorptive strength of the 
      antigen and their specific surface area determined the adsorptive capacity of the 
      antigen. In addition, surface hydroxyl contents had an impact on the stability of 
      the adsorbed antigen. By engineering the key intrinsic characteristics of 
      aluminum-based adjuvants, the antigen adsorption behavior with the aluminum 
      adjuvant could be regulated. This will facilitate the design of vaccine 
      formulations to optimize the adsorption and stability of the antigen in vaccine.
CI  - (c) 2022. The Author(s).
FAU - Yu, Ge
AU  - Yu G
AD  - State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 
      Linggong Road, 116024, Dalian, China.
AD  - School of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 
      116024, Dalian, China.
FAU - Liang, Zhihui
AU  - Liang Z
AD  - State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 
      Linggong Road, 116024, Dalian, China.
AD  - School of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 
      116024, Dalian, China.
FAU - Yu, Zilan
AU  - Yu Z
AD  - School of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 
      116024, Dalian, China.
FAU - Li, Min
AU  - Li M
AD  - State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 
      Linggong Road, 116024, Dalian, China.
AD  - School of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 
      116024, Dalian, China.
FAU - Yang, Wenqi
AU  - Yang W
AD  - State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 
      Linggong Road, 116024, Dalian, China.
AD  - School of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 
      116024, Dalian, China.
FAU - Zhang, Yawei
AU  - Zhang Y
AD  - School of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 
      116024, Dalian, China.
FAU - Zhao, Yuhang
AU  - Zhao Y
AD  - School of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 
      116024, Dalian, China.
FAU - Yang, Cheng
AU  - Yang C
AD  - School of Chemistry, Dalian University of Technology, 2 Linggong Road, 116024, 
      Dalian, China.
FAU - Xue, Changying
AU  - Xue C
AD  - School of Bioengineering, Dalian University of Technology, 2 Linggong Road, 
      116024, Dalian, China.
FAU - Shi, Li
AU  - Shi L
AD  - Immune Path Biotechnology (Su Zhou) Co., Ltd., Building A, 8 Chang Ting Road, 
      DaXin Industry Park, 215151, Su Zhou, Jiang Su, China.
FAU - Sun, Bingbing
AU  - Sun B
AUID- ORCID: 0000-0002-5444-5078
AD  - State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 
      Linggong Road, 116024, Dalian, China. bingbingsun@dlut.edu.cn.
AD  - School of Chemical Engineering, Dalian University of Technology, 2 Linggong Road, 
      116024, Dalian, China. bingbingsun@dlut.edu.cn.
LA  - eng
GR  - 31870919/National Natural Science Foundation of China (National Science 
      Foundation of China)/
GR  - 21876017/National Natural Science Foundation of China (National Science 
      Foundation of China)/
GR  - No. XLYC1807113/Natural Science Foundation of Liaoning Province (Liaoning 
      Provincial Natural Science Foundation)/
PT  - Journal Article
DEP - 20220623
PL  - England
TA  - NPJ Vaccines
JT  - NPJ vaccines
JID - 101699863
PMC - PMC9226065
COIS- The authors declare no competing interests.
EDAT- 2022/06/24 06:00
MHDA- 2022/06/24 06:01
PMCR- 2022/06/23
CRDT- 2022/06/23 23:21
PHST- 2022/01/21 00:00 [received]
PHST- 2022/05/13 00:00 [accepted]
PHST- 2022/06/23 23:21 [entrez]
PHST- 2022/06/24 06:00 [pubmed]
PHST- 2022/06/24 06:01 [medline]
PHST- 2022/06/23 00:00 [pmc-release]
AID - 10.1038/s41541-022-00495-9 [pii]
AID - 495 [pii]
AID - 10.1038/s41541-022-00495-9 [doi]
PST - epublish
SO  - NPJ Vaccines. 2022 Jun 23;7(1):62. doi: 10.1038/s41541-022-00495-9.