PMID- 35741774
OWN - NLM
STAT- MEDLINE
DCOM- 20220627
LR  - 20220924
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 13
IP  - 6
DP  - 2022 Jun 3
TI  - Molecular Cytogenetics Reveals Mosaicism in Human Umbilical Vein Endothelial 
      Cells.
LID - 10.3390/genes13061012 [doi]
LID - 1012
AB  - Primary human umbilical vein endothelial cells (HUVECs) are consistently the most 
      reliable in vitro model system for studying the inner lining of blood and 
      lymphatic vessels or the endothelium. Primary human cells originate from freshly 
      isolated tissues without genetic manipulation and generally show a modal number 
      of 46 chromosomes with no structural alterations, at least during early passages. 
      We investigated the cytogenetic integrity of HUVECs with conventional (G-banding) 
      and molecular cytogenetic methods (spectral karyotyping (SKY) and fluorescence in 
      situ hybridization (FISH)). Our G-band data shows two X-chromosomes, confirming 
      these HUVECs originate from a female donor. Notably, some cells consistently 
      exhibit an unfamiliar banding pattern on one X chromosome toward the distal end 
      of the long arm (Xq). Our FISH analysis confirms that approximately 50% of these 
      HUVECs have a deletion of the Xq terminal region. SKY analysis indicates that the 
      deleted region is apparently not integrated into any other chromosome. Finally, 
      we demonstrated the presence of a similar Xq deletion in the daughter cell line, 
      EA.hy926, which was generated by fusing HUVECs with A549 (a thioguanine-resistant 
      clone of adenocarcinomic human alveolar basal epithelial cells). These findings 
      will advance comprehension of HUVECs biology and will augment future endothelial 
      studies.
FAU - Binz, Regina L
AU  - Binz RL
AUID- ORCID: 0000-0002-4689-3073
AD  - Division of Radiation Health, Department of Pharmaceutical Sciences, College of 
      Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR 72205, 
      USA.
FAU - Pathak, Rupak
AU  - Pathak R
AUID- ORCID: 0000-0002-6239-301X
AD  - Division of Radiation Health, Department of Pharmaceutical Sciences, College of 
      Pharmacy, University of Arkansas for Medical Sciences, Little Rock, AR 72205, 
      USA.
LA  - eng
GR  - P20 GM109005/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20220603
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
SB  - IM
MH  - Cytogenetics
MH  - Female
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Karyotyping
MH  - *Mosaicism
PMC - PMC9222953
OTO - NOTNLM
OT  - G-banding
OT  - chromosomal aberrations
OT  - endothelial cells
OT  - fluorescence in situ hybridization
OT  - spectral karyotyping
COIS- The authors declare no conflict of interest.
EDAT- 2022/06/25 06:00
MHDA- 2022/06/28 06:00
PMCR- 2022/06/03
CRDT- 2022/06/24 01:13
PHST- 2022/05/09 00:00 [received]
PHST- 2022/05/31 00:00 [revised]
PHST- 2022/06/02 00:00 [accepted]
PHST- 2022/06/24 01:13 [entrez]
PHST- 2022/06/25 06:00 [pubmed]
PHST- 2022/06/28 06:00 [medline]
PHST- 2022/06/03 00:00 [pmc-release]
AID - genes13061012 [pii]
AID - genes-13-01012 [pii]
AID - 10.3390/genes13061012 [doi]
PST - epublish
SO  - Genes (Basel). 2022 Jun 3;13(6):1012. doi: 10.3390/genes13061012.