PMID- 35743241
OWN - NLM
STAT- MEDLINE
DCOM- 20220627
LR  - 20220716
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 12
DP  - 2022 Jun 18
TI  - Unraveling the DNA Methylation in the rDNA Foci in Mutagen-Induced Brachypodium 
      distachyon Micronuclei.
LID - 10.3390/ijms23126797 [doi]
LID - 6797
AB  - Many years have passed since micronuclei were first observed then accepted as an 
      indicator of the effect of mutagens. However, the possible mechanisms of their 
      formation and elimination from the cell are still not fully understood. Various 
      stresses, including mutagens, can alter gene expression through changes in DNA 
      methylation in plants. In this study we demonstrate for the first time DNA 
      methylation in the foci of 5S and 35S rDNA sequences in individual Brachypodium 
      distachyon micronuclei that are induced by mutagenic treatment with maleic acid 
      hydrazide (MH). The impact of MH on global epigenetic modifications in nuclei and 
      micronuclei has been studied in plants before; however, no in situ analyses of 
      DNA methylation in specific DNA sequence sites are known. To address this 
      problem, we used sequential immunodetection of 5-methylcytosine and fluorescence 
      in situ hybridization (FISH) with 5S and 25S rDNA probes on the non-dividing 
      cells of B. distachyon. Such investigations into the presence or absence of DNA 
      methylation within specific DNA sequences are extremely important in plant 
      mutagenesis in the light of altering gene expression.
FAU - Bara-Halama, Adrianna W
AU  - Bara-Halama AW
AD  - Plant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, 
      University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, Poland.
FAU - Idziak-Helmcke, Dominika
AU  - Idziak-Helmcke D
AUID- ORCID: 0000-0002-0713-2466
AD  - Plant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, 
      University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, Poland.
FAU - Kwasniewska, Jolanta
AU  - Kwasniewska J
AUID- ORCID: 0000-0002-8965-8727
AD  - Plant Cytogenetics and Molecular Biology Group, Faculty of Natural Sciences, 
      University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, Poland.
LA  - eng
GR  - This work was supported by the funds granted under the Research Excellence 
      Initiative of the University of Silesia in Katowice./
PT  - Journal Article
DEP - 20220618
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Mutagens)
RN  - TQ7L3FCV66 (Maleic Hydrazide)
SB  - IM
MH  - *Brachypodium/genetics
MH  - Chromosomes, Plant
MH  - DNA Methylation
MH  - DNA, Plant/genetics
MH  - DNA, Ribosomal/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Maleic Hydrazide/pharmacology
MH  - Mutagens/toxicity
MH  - Plants/genetics
PMC - PMC9224279
OTO - NOTNLM
OT  - Brachypodium distachyon
OT  - DNA methylation
OT  - FISH
OT  - maleic acid hydrazide
OT  - micronuclei
OT  - rDNA
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses or interpretation of the data; 
      in the writing of the manuscript; or in the decision to publish the results.
EDAT- 2022/06/25 06:00
MHDA- 2022/06/28 06:00
PMCR- 2022/06/18
CRDT- 2022/06/24 01:23
PHST- 2022/05/17 00:00 [received]
PHST- 2022/06/15 00:00 [revised]
PHST- 2022/06/17 00:00 [accepted]
PHST- 2022/06/24 01:23 [entrez]
PHST- 2022/06/25 06:00 [pubmed]
PHST- 2022/06/28 06:00 [medline]
PHST- 2022/06/18 00:00 [pmc-release]
AID - ijms23126797 [pii]
AID - ijms-23-06797 [pii]
AID - 10.3390/ijms23126797 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Jun 18;23(12):6797. doi: 10.3390/ijms23126797.