PMID- 35745065
OWN - NLM
STAT- MEDLINE
DCOM- 20220627
LR  - 20220716
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 12
DP  - 2022 Jun 20
TI  - Graphene Oxide-Sensitized Surface Plasmon Resonance Biosensor of Porcine 
      Reproductive and Respiratory Syndrome Virus.
LID - 10.3390/molecules27123942 [doi]
LID - 3942
AB  - Biosensor analysis based on the surface plasmon resonance (SPR) phenomenon 
      enables label-free, highly sensitive analyte detection without prior sample 
      purification or processing. However, potential applications of SPR biosensors in 
      virus detection in biological samples remain to be explored. Owing to its 
      excellent biocompatibility and abundance of hydroxyl and carboxyl functional 
      groups, graphene oxide (GO) has been widely used as a biosensor of proteins and 
      metal ions in living cells. The present work explored the effect of GO 
      modification on the sensitivity of an SPR biosensor and used a GO-modified sensor 
      to detect porcine reproductive and respiratory syndrome virus in cell culture, as 
      shown. The GO modification markedly enhanced the sensitivity of the Fourier 
      transform SPR sensor and enabled linear detection of porcine reproductive and 
      respiratory syndrome virus (PRRSV) with a multiplicity of infection in the range 
      0.2-1.7 (R(2) = 0.998). Such a GO-modified sensor provides a promising 
      alternative for virus detection.
FAU - Liu, Xuemei
AU  - Liu X
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Veterinary Medicine, Shandong Agricultural University, 
      Taian 271018, China.
AD  - College of Chemistry and Material Science, Shandong Agricultural University, 
      Taian 271018, China.
FAU - Xu, Chao
AU  - Xu C
AD  - College of Chemistry and Material Science, Shandong Agricultural University, 
      Taian 271018, China.
FAU - Fu, Chunyu
AU  - Fu C
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Veterinary Medicine, Shandong Agricultural University, 
      Taian 271018, China.
FAU - Xia, Dongfang
AU  - Xia D
AD  - College of Chemistry and Material Science, Shandong Agricultural University, 
      Taian 271018, China.
FAU - Wang, Fuchao
AU  - Wang F
AD  - College of Chemistry and Material Science, Shandong Agricultural University, 
      Taian 271018, China.
FAU - Yin, Hongzong
AU  - Yin H
AD  - College of Chemistry and Material Science, Shandong Agricultural University, 
      Taian 271018, China.
FAU - Peng, Jun
AU  - Peng J
AUID- ORCID: 0000-0003-3900-4183
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, College of Veterinary Medicine, Shandong Agricultural University, 
      Taian 271018, China.
LA  - eng
GR  - 31672587/National Natural Science Foundation of China/
GR  - 2020CXGC010801/Great scientific and technological innovation projects in Shandong 
      province of China/
GR  - 2021LZGC001/Agricultural improved varieties project in Shandong province of 
      China/
PT  - Journal Article
DEP - 20220620
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - *Biosensing Techniques
MH  - *Graphite
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Surface Plasmon Resonance
PMC - PMC9229610
OTO - NOTNLM
OT  - FT-SPR
OT  - GO
OT  - PRRSV
OT  - sensitization
COIS- The authors declare no conflict of interest.
EDAT- 2022/06/25 06:00
MHDA- 2022/06/28 06:00
PMCR- 2022/06/20
CRDT- 2022/06/24 01:33
PHST- 2022/05/24 00:00 [received]
PHST- 2022/06/13 00:00 [revised]
PHST- 2022/06/13 00:00 [accepted]
PHST- 2022/06/24 01:33 [entrez]
PHST- 2022/06/25 06:00 [pubmed]
PHST- 2022/06/28 06:00 [medline]
PHST- 2022/06/20 00:00 [pmc-release]
AID - molecules27123942 [pii]
AID - molecules-27-03942 [pii]
AID - 10.3390/molecules27123942 [doi]
PST - epublish
SO  - Molecules. 2022 Jun 20;27(12):3942. doi: 10.3390/molecules27123942.