PMID- 35745755
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1999-4923 (Print)
IS  - 1999-4923 (Electronic)
IS  - 1999-4923 (Linking)
VI  - 14
IP  - 6
DP  - 2022 May 31
TI  - Polyphenols-Rich Fraction from Annona muricata Linn. Leaves Attenuates Oxidative 
      and Inflammatory Responses in Neutrophils, Macrophages, and Experimental Lung 
      Injury.
LID - 10.3390/pharmaceutics14061182 [doi]
LID - 1182
AB  - Annona muricata Linn. is a common plant found in the warmest regions of South and 
      Central America and its use in traditional medicine has been reported for the 
      treatment of various illnesses. In the current study, we investigate the 
      antioxidant and anti-inflammatory activities of crude extract and fractions from 
      A. muricata L. leaves in isolated murine phagocytic immune cells as well as 
      experimental LPS-induced acute lung injury (ALI). In a luminol-dependent 
      chemiluminescence assay, we showed that ethyl acetate (EtOAc.f) and n-butanol 
      (BuOH.f) fractions-both rich in polyphenols-reduced the generation of reactive 
      oxygen species (ROS) by neutrophils stimulated with opsonized zymosan; similar 
      results were found in culture of bone marrow-derived macrophages (BMDMs). By 
      evaluating anti-inflammatory activity in BMDMs, EtOAc.f and BuOH.f reduced 
      secretion of IL-6 and expression of the co-stimulatory molecule CD40. 
      Furthermore, in LPS-induced ALI, oral administration of EtOAc.f reduced 
      myeloperoxidase (MPO) activity in lung tissue. In addition, on a mechanism 
      dependent on glutathione levels, the oxidative damage was also attenuated. These 
      findings revealed direct antioxidant and anti-inflammatory activities of 
      polyphenols-rich fractions of A. muricata L. leaves on neutrophils and 
      macrophages. Moreover, the reduced oxidative damage and levels of inflammatory 
      markers in experimental ALI suggest that these fractions might be explored for 
      the development of new therapies for inflammatory conditions.
FAU - Saraiva, Andre Lopes
AU  - Saraiva AL
AD  - Institute of Biotechnology, Federal University of Uberlandia, Rua Acre s/n, Bloco 
      2E, Uberlandia 38400-902, MG, Brazil.
FAU - Justino, Allisson Benatti
AU  - Justino AB
AD  - Institute of Biotechnology, Federal University of Uberlandia, Rua Acre s/n, Bloco 
      2E, Uberlandia 38400-902, MG, Brazil.
FAU - Franco, Rodrigo Rodrigues
AU  - Franco RR
AUID- ORCID: 0000-0002-9138-311X
AD  - Institute of Biotechnology, Federal University of Uberlandia, Rua Acre s/n, Bloco 
      2E, Uberlandia 38400-902, MG, Brazil.
FAU - Silva, Heitor Cappato Guerra
AU  - Silva HCG
AD  - Institute of Biotechnology, Federal University of Uberlandia, Rua Acre s/n, Bloco 
      2E, Uberlandia 38400-902, MG, Brazil.
FAU - Arruda, Felipe Dos Santos
AU  - Arruda FDS
AUID- ORCID: 0000-0002-6775-390X
AD  - Department of Bioscience and Technology, Institute of Tropical Pathology and 
      Public Health, Federal University of Goias, Rua 235, Setor Leste Universitario, 
      Goiania 74605-050, GO, Brazil.
FAU - Klein, Sandra Gabriela
AU  - Klein SG
AD  - Rodent Vivarium Network (REBIR), Dean of Research and Graduate Studies, Federal 
      University of Uberlandia, Rua Ceara s/n, Bloco 4U, Uberlandia 38405-315, MG, 
      Brazil.
FAU - Celes, Mara Rubia Nunes
AU  - Celes MRN
AUID- ORCID: 0000-0002-4733-007X
AD  - Department of Bioscience and Technology, Institute of Tropical Pathology and 
      Public Health, Federal University of Goias, Rua 235, Setor Leste Universitario, 
      Goiania 74605-050, GO, Brazil.
FAU - Goulart, Luiz Ricardo
AU  - Goulart LR
FAU - Espindola, Foued Salmen
AU  - Espindola FS
AUID- ORCID: 0000-0002-6937-1411
AD  - Institute of Biotechnology, Federal University of Uberlandia, Rua Acre s/n, Bloco 
      2E, Uberlandia 38400-902, MG, Brazil.
LA  - eng
GR  - 465669/ 2014-0/Institutos Nacionais de Ciencia e Tecnologia/
GR  - APQ-01856-14/Fundacao de Amparo a Pesquisa do Estado de Minas Gerais/
PT  - Journal Article
DEP - 20220531
PL  - Switzerland
TA  - Pharmaceutics
JT  - Pharmaceutics
JID - 101534003
PMC - PMC9228609
OTO - NOTNLM
OT  - Annonaceae
OT  - acute lung injury
OT  - macrophages
OT  - natural products
OT  - neutrophils
OT  - reactive oxygen species
COIS- The authors declare that there are no conflict of interest.
EDAT- 2022/06/25 06:00
MHDA- 2022/06/25 06:01
PMCR- 2022/05/31
CRDT- 2022/06/24 01:37
PHST- 2022/03/08 00:00 [received]
PHST- 2022/05/23 00:00 [revised]
PHST- 2022/05/26 00:00 [accepted]
PHST- 2022/06/24 01:37 [entrez]
PHST- 2022/06/25 06:00 [pubmed]
PHST- 2022/06/25 06:01 [medline]
PHST- 2022/05/31 00:00 [pmc-release]
AID - pharmaceutics14061182 [pii]
AID - pharmaceutics-14-01182 [pii]
AID - 10.3390/pharmaceutics14061182 [doi]
PST - epublish
SO  - Pharmaceutics. 2022 May 31;14(6):1182. doi: 10.3390/pharmaceutics14061182.