PMID- 35746618 OWN - NLM STAT- MEDLINE DCOM- 20220627 LR - 20220722 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 14 IP - 6 DP - 2022 May 25 TI - Secretory Profile of Adipose-Tissue-Derived Mesenchymal Stem Cells from Cats with Calicivirus-Positive Severe Chronic Gingivostomatitis. LID - 10.3390/v14061146 [doi] LID - 1146 AB - The feline calicivirus (FCV) causes infections in cats all over the world and seems to be related to a broad variety of clinical presentations, such as feline chronic gingivostomatitis (FCGS), a severe oral pathology in cats. Although its etiopathogeny is largely unknown, FCV infection is likely to be a main predisposing factor for developing this pathology. During recent years, new strategies for treating FCGS have been proposed, based on the use of mesenchymal stem cells (MSC) and their regenerative and immunomodulatory properties. The main mechanism of action of MSC seems to be paracrine, due to the secretion of many biomolecules with different biological functions (secretome). Currently, several pathologies in humans have been shown to be related to functional alterations of the patient's MSCs. However, the possible roles that altered MSCs might have in different diseases, including virus-mediated diseases, remain unknown. We have recently demonstrated that the exosomes produced by the adipose-tissue-derived MSCs (fAd-MSCs) from cats suffering from FCV-positive severe and refractory FCGS showed altered protein contents. Based on these findings, the goal of this work was to analyze the proteomic profile of the secretome produced by feline adipose-tissue-derived MSCs (fAd-MSCs) from FCV-positive patients with FCGS, in order to identify differences between them and to increase our knowledge of the etiopathogenesis of this disease. We used high-resolution mass spectrometry and functional enrichment analysis with Gene Ontology to compare the secretomes produced by the fAd-MSCs of healthy and calicivirus-positive FCGS cats. We found that the fAd-MSCs from cats with FCGS had an increased expression of pro-inflammatory cytokines and an altered proteomic profile compared to the secretome produced by cells from healthy cats. These findings help us gain insight on the roles of MSCs and their possible relation to FCGS, and may be useful for selecting specific biomarkers and for identifying new therapeutic targets. FAU - Villatoro, Antonio J AU - Villatoro AJ AD - Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, Biomedical Research Institute of Malaga (IBIMA), University of Malaga, 29071 Malaga, Spain. AD - Grupo Ynmun, Inmunologia Clinica y Terapia Celular (IMMUNESTEM), 29071 Malaga, Spain. FAU - Martin-Astorga, Maria Del Carmen AU - Martin-Astorga MDC AUID- ORCID: 0000-0003-4784-9944 AD - Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, Biomedical Research Institute of Malaga (IBIMA), University of Malaga, 29071 Malaga, Spain. FAU - Alcoholado, Cristina AU - Alcoholado C AUID- ORCID: 0000-0002-6238-2521 AD - Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, Biomedical Research Institute of Malaga (IBIMA), University of Malaga, 29071 Malaga, Spain. FAU - Kazantseva, Liliya AU - Kazantseva L AD - Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, Biomedical Research Institute of Malaga (IBIMA), University of Malaga, 29071 Malaga, Spain. FAU - Cardenas, Casimiro AU - Cardenas C AUID- ORCID: 0000-0001-6259-8296 AD - Research Support Central Services (SCAI) of the University of Malaga, 29071 Malaga, Spain. FAU - Farinas, Fernando AU - Farinas F AD - Grupo Ynmun, Spanish Association for the Research in Immunological and Infectious Diseases, 29071 Malaga, Spain. FAU - Becerra, Jose AU - Becerra J AUID- ORCID: 0000-0002-4849-8931 AD - Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, Biomedical Research Institute of Malaga (IBIMA), University of Malaga, 29071 Malaga, Spain. AD - Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain. FAU - Visser, Rick AU - Visser R AD - Laboratory of Bioengineering and Tissue Regeneration, Department of Cell Biology, Genetics and Physiology, Biomedical Research Institute of Malaga (IBIMA), University of Malaga, 29071 Malaga, Spain. AD - Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220525 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 146-14-5 (Flavin-Adenine Dinucleotide) SB - IM MH - Animals MH - *Calicivirus, Feline MH - *Cat Diseases/therapy MH - Cats MH - Flavin-Adenine Dinucleotide MH - Humans MH - *Mesenchymal Stem Cells MH - Proteomics MH - *Stomatitis PMC - PMC9228153 OTO - NOTNLM OT - bioinformatics OT - cats OT - feline chronic gingivostomatitis OT - immunoassay OT - mesenchymal stem cells OT - secretome OT - ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) COIS- The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. EDAT- 2022/06/25 06:00 MHDA- 2022/06/28 06:00 PMCR- 2022/05/25 CRDT- 2022/06/24 01:42 PHST- 2022/03/31 00:00 [received] PHST- 2022/05/14 00:00 [revised] PHST- 2022/05/22 00:00 [accepted] PHST- 2022/06/24 01:42 [entrez] PHST- 2022/06/25 06:00 [pubmed] PHST- 2022/06/28 06:00 [medline] PHST- 2022/05/25 00:00 [pmc-release] AID - v14061146 [pii] AID - viruses-14-01146 [pii] AID - 10.3390/v14061146 [doi] PST - epublish SO - Viruses. 2022 May 25;14(6):1146. doi: 10.3390/v14061146.