PMID- 35746666
OWN - NLM
STAT- MEDLINE
DCOM- 20220627
LR  - 20220721
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 14
IP  - 6
DP  - 2022 May 31
TI  - Evolution Characterization and Pathogenicity of a Porcine Reproductive and 
      Respiratory Syndrome Virus Isolate from a Pig Farm in Shandong Province, China.
LID - 10.3390/v14061194 [doi]
LID - 1194
AB  - In recent years, porcine reproductive and respiratory syndrome virus (PRRSV) 
      strains have been experiencing extensive recombination in Chinese swine farms. 
      This recombination usually happens in NADC30/34 strains and highly pathogenic 
      (HP) PRRSV strains. This study identified a new PRRSV isolate that shared 99% and 
      99.1% nucleotide identity with CH-1a and CH-1R at the genomic level, 
      respectively. After purification by viral plaque assay, this isolate was named 
      PRRSV CSR1801. The isolate did not experience any recombination with other PRRSV 
      strains common in swine herd epidemics in China, which means it still maintains 
      the stable features of the classical PRRSV strain and did not easily recombine 
      with other PRRSV strains. Further analysis of the pathogenicity of the PRRSV 
      isolate CSR1801 was performed in piglets. The results indicated that none of the 
      inoculated piglets showed the typical clinical manifestations of PRRS, which 
      presented with runny noses, rough back hair, rectal temperatures always below 
      40.5  degrees C, and no deaths. Additionally, no obvious histopathological lesions such 
      as severe interstitial pneumonia could be observed in the lungs of the piglets. 
      Hence, the PRRSV isolate CSR1801 should be classified as a classical-like PRRSV 
      strain. This classical PRRSV strain showed genetic stability and maintained low 
      pathogenicity. This study may provide new clues for further understanding the 
      genetic evolution and pathogenicity of PRRSV and may also be an important 
      reference for the prevention and control of PRRS in swine farms.
FAU - Xu, Yulin
AU  - Xu Y
AD  - College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271000, 
      China.
FAU - Ji, Xiaojing
AU  - Ji X
AD  - College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271000, 
      China.
FAU - Fu, Chunyu
AU  - Fu C
AD  - College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271000, 
      China.
FAU - Hu, Dong
AU  - Hu D
AD  - College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271000, 
      China.
FAU - Pang, Heng
AU  - Pang H
AD  - College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271000, 
      China.
FAU - Wang, Tingting
AU  - Wang T
AD  - College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271000, 
      China.
FAU - Li, Chuangang
AU  - Li C
AD  - College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271000, 
      China.
FAU - Wang, Gang
AU  - Wang G
AD  - College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271000, 
      China.
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention of China, East China Scientific Experimental Station of Animal 
      Pathogen Biology of Ministry of Agriculture and Rural Affairs of China, Tai'an 
      271000, China.
FAU - Peng, Jun
AU  - Peng J
AUID- ORCID: 0000-0003-3900-4183
AD  - College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271000, 
      China.
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention of China, East China Scientific Experimental Station of Animal 
      Pathogen Biology of Ministry of Agriculture and Rural Affairs of China, Tai'an 
      271000, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220531
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
SB  - IM
MH  - Animals
MH  - China/epidemiology
MH  - Farms
MH  - Genome, Viral
MH  - Phylogeny
MH  - *Porcine Reproductive and Respiratory Syndrome/epidemiology/genetics
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - Swine
MH  - Virulence
PMC - PMC9228802
OTO - NOTNLM
OT  - genetic stability
OT  - low pathogenicity
OT  - novel classical isolate
OT  - porcine reproductive and respiratory syndrome virus
COIS- The authors declare no conflict of interest.
EDAT- 2022/06/25 06:00
MHDA- 2022/06/28 06:00
PMCR- 2022/05/31
CRDT- 2022/06/24 01:43
PHST- 2022/05/03 00:00 [received]
PHST- 2022/05/27 00:00 [revised]
PHST- 2022/05/29 00:00 [accepted]
PHST- 2022/06/24 01:43 [entrez]
PHST- 2022/06/25 06:00 [pubmed]
PHST- 2022/06/28 06:00 [medline]
PHST- 2022/05/31 00:00 [pmc-release]
AID - v14061194 [pii]
AID - viruses-14-01194 [pii]
AID - 10.3390/v14061194 [doi]
PST - epublish
SO  - Viruses. 2022 May 31;14(6):1194. doi: 10.3390/v14061194.