PMID- 35753740
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220626
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 45
IP  - 11
DP  - 2020 Nov 28
TI  - Effect of salvianolic acid A on the proliferation and apoptosis in esophageal 
      cancer cells and the underlying mechanisms.
PG  - 1269-1275
LID - 1672-7347(2020)11-1269-07 [pii]
LID - 10.11817/j.issn.1672-7347.2020.190291 [doi]
AB  - OBJECTIVES: To explore the effect of salvianolic acid A (SalA) on the 
      proliferation and apoptosis in esophageal cancer cell line KYSE-150 and the 
      possible mechanisms. METHODS: The esophageal cancer cells were randomly divided 
      into 4 groups: a control group, a 10 mumol/L SalA group, a 25 mumol/L SalA group, 
      and a 50 mumol/L SalA group. Cell counting kit-8 (CCK-8) was used to detect the 
      cell proliferation activity. Flow cytometry was used to detect cell cycle 
      distribution and cell apoptosis rate. Western blotting was used to detect the 
      protein expression of cell proliferation maker Ki-67, cyclin D1, cyclin-dependent 
      kinase 4 (CDK4), CDK6, B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X (Bax), 
      cleaved-caspase-9, cleaved-caspase-3, phosphatidylinositol 3-kinase (PI3K), 
      phosphorylated protein kinase B (p-Akt) and mechanistic target of rapamycin 
      (mTOR). RESULTS: Compared with the control group, the cell proliferation activity 
      was significantly reduced (P<0.01); the cells in the G(1) phase were 
      significantly increased, and the S phase cells were significantly reduced (both 
      P<0.01); the cell apoptosis rate was significantly increased (P<0.01) in the SalA 
      groups at different concentration; the expression levels of Ki-67, cyclin D1, 
      CDK4, CDK6, Bcl-2, PI3K, p-Akt and mTOR were decreased significantly, but the 
      expression levels of p21, Bax, cl-caspase-9 and cl-caspase-3 were increased 
      significantly in the SalA groups at different concentration (all P<0.01). 
      CONCLUSIONS: SalA can inhibit the proliferation and induce G(1) phase arrest and 
      apoptosis in the esophageal cancer cell line KYSE-150, which may be related to 
      the activation of PI3K/Akt/mTOR signal pathway.
FAU - Yin, Xiaofei
AU  - Yin X
AD  - Department of Gastroenterology, Nanshan People's Hospital, Shenzhen Guangdong 
      518052, China. yinxiaofei1221@163.com.
FAU - Feng, Yanyan
AU  - Feng Y
AD  - Department of Gastroenterology, Nanshan People's Hospital, Shenzhen Guangdong 
      518052, China.
FAU - Kang, Wenquan
AU  - Kang W
AD  - Department of Gastroenterology, Nanshan People's Hospital, Shenzhen Guangdong 
      518052, China.
LA  - eng
LA  - chi
GR  - 201775320/the Natural Science Foundation of Guangdong Provience, China/
PT  - Journal Article
TT  - 丹酚酸A对食管癌细胞增殖与凋亡的影响及其机制.
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
SB  - IM
OTO - NOTNLM
OT  - apoptosis
OT  - cell cycle arrest
OT  - esophageal cancer
OT  - proliferation
OT  - salvianolic acid A
EDAT- 2020/11/28 00:00
MHDA- 2020/11/28 00:01
CRDT- 2022/06/26 21:22
PHST- 2022/06/26 21:22 [entrez]
PHST- 2020/11/28 00:00 [pubmed]
PHST- 2020/11/28 00:01 [medline]
AID - 1672-7347(2020)11-1269-07 [pii]
AID - 10.11817/j.issn.1672-7347.2020.190291 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2020 Nov 28;45(11):1269-1275. doi: 
      10.11817/j.issn.1672-7347.2020.190291.