PMID- 35758657
OWN - NLM
STAT- MEDLINE
DCOM- 20220629
LR  - 20230825
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 184
DP  - 2022 Jun 10
TI  - Manual Construction of a Tissue Microarray using the Tape Method and a Handheld 
      Microarrayer.
LID - 10.3791/63086 [doi]
AB  - The tissue microarray (TMA) is an important research tool in which many formalin 
      fixed paraffin embedded (FFPE) samples can be represented in a single paraffin 
      block. This is achieved by using tissue cores extracted from the region of 
      interest of different donor FFPE blocks and arranging them into a single TMA 
      paraffin block. Once constructed, sections from the completed TMA can be used to 
      perform immunohistochemistry, chromogenic, fluorescence in situ hybridization 
      (FISH) and RNA ISH studies to assess protein expression as well as genomic and 
      transcriptional alterations in many samples simultaneously, thus minimizing 
      tissue usage and reducing reagent costs. There are several different TMA 
      construction techniques. One of the most common construction methods is the 
      recipient method, which works best with cores of the same length for which a 
      minimum length of 4 mm is recommended. Unfortunately, tissue blocks can be 
      heavily resected during the diagnostic process, frequently resulting in 
      "non-ideal" donor block thicknesses of less than 4 mm. The current article and 
      video focus on the double-sided adhesive tape method; an alternative manual, low 
      cost, easy to use, and rapid method to construct low density (<50 cores) TMAs 
      that is highly compatible with these non-ideal donor blocks. This protocol 
      provides a step-by-step guide on how to construct a TMA using this method, with a 
      focus on the critical importance of pathological review and post construction 
      validation.
FAU - Wisner, Lee
AU  - Wisner L
AD  - Department of Research, Mayo Clinic.
FAU - Larsen, Brandon
AU  - Larsen B
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic.
FAU - Maguire, Alanna
AU  - Maguire A
AD  - Department of Research, Mayo Clinic; Maguire.Alanna@mayo.edu.
LA  - eng
GR  - UM1 CA181255/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Video-Audio Media
DEP - 20220610
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Adrenergic Agents)
RN  - 0 (Amines)
RN  - 8002-74-2 (Paraffin)
SB  - IM
MH  - Adrenergic Agents
MH  - Amines
MH  - *Genomics
MH  - In Situ Hybridization, Fluorescence
MH  - *Paraffin
PMC - PMC10448997
MID - NIHMS1914858
EDAT- 2022/06/28 06:00
MHDA- 2022/06/30 06:00
PMCR- 2023/08/24
CRDT- 2022/06/27 09:03
PHST- 2022/06/27 09:03 [entrez]
PHST- 2022/06/28 06:00 [pubmed]
PHST- 2022/06/30 06:00 [medline]
PHST- 2023/08/24 00:00 [pmc-release]
AID - 10.3791/63086 [doi]
PST - epublish
SO  - J Vis Exp. 2022 Jun 10;(184):10.3791/63086. doi: 10.3791/63086.