PMID- 35761623
OWN - NLM
STAT- MEDLINE
DCOM- 20220629
LR  - 20220908
IS  - 1873-7145 (Electronic)
IS  - 0963-9969 (Linking)
VI  - 157
DP  - 2022 Jul
TI  - Effect of chlorine sanitizer on metabolic responses of Escherichia coli biofilms 
      "big six" during cross-contamination from abiotic surface to sponge cake.
PG  - 111361
LID - S0963-9969(22)00418-5 [pii]
LID - 10.1016/j.foodres.2022.111361 [doi]
AB  - The effect of chlorine on Escherichia coli biofilm O157:H7 are well established; 
      however, the effect on biofilm adhesion to food as well as the six emerging E. 
      coli serotypes ("big six") have not been fully understood. Chlorine sanitization 
      with 1-min 100 mg/L was applied against seven pathogenic E. coli (O111, O121:H19, 
      O45:H2, O26:H11, O103:H11, O145, and O157:H7) biofilms on high-density 
      polyethylene (HDPE) and stainless steel (SS) coupons, respectively. Using sponge 
      cake as a food model, the adhesion behavior was evaluated by comparison of 
      bacteria transfer rate before and after treatment. Besides, the metabolic 
      profiles of biofilms were analyzed by nuclear magnetic resonance (NMR) 
      spectrometer. A significant decrease in transfer rate (79% decline on SS and 33% 
      decline on HDPE) was recorded as well as the distinctive pattern between SS and 
      HDPE coupons was also noticed, with a low population (6-7 log CFU/coupon) 
      attached and low survivals (0-3 log CFU/coupon) upon chlorine on SS, while high 
      population (7-8 log CFU/coupon) attached and high survivals (5-7 log CFU/coupon) 
      on HDPE. Moreover, O121:H19 and O26:H11 demonstrated the highest resistance to 
      chlorine with the least metabolic status and pathways affected. O103:H11, O145, 
      and O111 followed similar metabolic patterns on both surfaces. Distinct metabolic 
      patterns were found in O45:H2 and O157:H7, where the former had more affected 
      metabolic status and pathways on SS but less on HDPE, whereas the latter showed 
      an opposite trend. Overall, a potential contamination source of STEC infection in 
      flour products was demonstrated and metabolic changes induced by chlorine were 
      revealed by NMR-based metabolomics, which provides insights to avoid "big six" 
      biofilms contamination in food.
CI  - Copyright (c) 2022. Published by Elsevier Ltd.
FAU - Lin, Zejia
AU  - Lin Z
AD  - Department of Food Science & Technology, National University of Singapore, 
      Singapore 117542, Singapore; National University of Singapore (Suzhou) Research 
      Institute, 377 Lin Quan Street, Suzhou Industrial Park, Suzhou, Jiangsu 215123, 
      PR China.
FAU - Chen, Tong
AU  - Chen T
AD  - Department of Food Science & Technology, National University of Singapore, 
      Singapore 117542, Singapore; National University of Singapore (Suzhou) Research 
      Institute, 377 Lin Quan Street, Suzhou Industrial Park, Suzhou, Jiangsu 215123, 
      PR China.
FAU - Zhou, Lehao
AU  - Zhou L
AD  - Department of Food Science & Technology, National University of Singapore, 
      Singapore 117542, Singapore; National University of Singapore (Suzhou) Research 
      Institute, 377 Lin Quan Street, Suzhou Industrial Park, Suzhou, Jiangsu 215123, 
      PR China.
FAU - Yang, Hongshun
AU  - Yang H
AD  - Department of Food Science & Technology, National University of Singapore, 
      Singapore 117542, Singapore; National University of Singapore (Suzhou) Research 
      Institute, 377 Lin Quan Street, Suzhou Industrial Park, Suzhou, Jiangsu 215123, 
      PR China. Electronic address: fstynghs@nus.edu.sg.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220513
PL  - Canada
TA  - Food Res Int
JT  - Food research international (Ottawa, Ont.)
JID - 9210143
RN  - 12597-68-1 (Stainless Steel)
RN  - 4R7X1O2820 (Chlorine)
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Biofilms
MH  - *Chlorine/metabolism/pharmacology
MH  - Colony Count, Microbial
MH  - *Escherichia coli O157
MH  - Food Microbiology
MH  - Polyethylene
MH  - Stainless Steel
OTO - NOTNLM
OT  - Biofilm
OT  - Escherichia coli
OT  - Flour
OT  - High-density polyethylene
OT  - Metabolomics
OT  - NMR
OT  - Sodium hypochlorite
OT  - Stainless steel
EDAT- 2022/06/29 06:00
MHDA- 2022/06/30 06:00
CRDT- 2022/06/28 01:02
PHST- 2022/02/17 00:00 [received]
PHST- 2022/04/19 00:00 [revised]
PHST- 2022/05/10 00:00 [accepted]
PHST- 2022/06/28 01:02 [entrez]
PHST- 2022/06/29 06:00 [pubmed]
PHST- 2022/06/30 06:00 [medline]
AID - S0963-9969(22)00418-5 [pii]
AID - 10.1016/j.foodres.2022.111361 [doi]
PST - ppublish
SO  - Food Res Int. 2022 Jul;157:111361. doi: 10.1016/j.foodres.2022.111361. Epub 2022 
      May 13.