PMID- 35761831 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220716 IS - 1687-966X (Print) IS - 1687-9678 (Electronic) VI - 2022 DP - 2022 TI - Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1beta Stimulation. PG - 7819234 LID - 10.1155/2022/7819234 [doi] LID - 7819234 AB - Thin endometrium (< 7 mm) could cause low clinical pregnancy, reduced live birth, increased spontaneous abortion, and decreased birth weight. However, the treatments for thin endometrium have not been well developed. In this study, we aim to determine the role of Pluronic F-127 (PF-127) encapsulation of human umbilical cord mesenchymal stem cells (hUC-MSCs) in the regeneration of thin endometrium and its underlying mechanism. Thin endometrium rat model was created by infusion of 95% ethanol. Thin endometrium modeled rat uterus were treated with saline, hUC-MSCs, PF-127, or hUC-MSCs plus PF-127 separately. Regenerated rat uterus was measured for gene expression levels of angiogenesis factors and histological morphology. Angiogenesis capacity of interleukin-1 beta (IL-1beta)-primed hUC-MSCs was monitored via quantitative polymerase chain reaction (q-PCR), Luminex assay, and tube formation assay. Decreased endometrium thickness and gland number and increased inflammatory factor IL-1beta were achieved in the thin endometrium rat model. Embedding of hUC-MSCs with PF-127 could prolong the hUC-MSCs retaining, which could further enhance endometrium thickness and gland number in the thin endometrium rat model via increasing angiogenesis capacity. Conditional medium derived from IL-1beta-primed hUC-MSCs increased the concentration of angiogenesis factors (basic fibroblast growth factor (bFGF), vascular endothelial growth factors (VEGF), and hepatocyte growth factor (HGF)). Improvement in the thickness, number of glands, and newly generated blood vessels could be achieved by uterus endometrium treatment with PF-127 and hUC-MSCs transplantation. Local IL-1beta stimulation-primed hUC-MSCs promoted the release of angiogenesis factors and may play a vital role on thin endometrium regeneration. CI - Copyright (c) 2022 Shuling Zhou et al. FAU - Zhou, Shuling AU - Zhou S AUID- ORCID: 0000-0003-3408-9501 AD - Jinxin Research Institute for Reproductive Medicine and Genetics, 66 Bisheng Road, Chengdu, 610066 Sichuan, China. AD - Department of Pathology and Provincial Key Laboratory of Infectious Diseases and Immunopathology, Collaborative and Creative Center, Shantou University Medical College, 22 Xinling Road, Shantou, 515041 Guangdong, China. FAU - Lei, Yu AU - Lei Y AD - Jinxin Research Institute for Reproductive Medicine and Genetics, 66 Bisheng Road, Chengdu, 610066 Sichuan, China. AD - Department of Pathology and Provincial Key Laboratory of Infectious Diseases and Immunopathology, Collaborative and Creative Center, Shantou University Medical College, 22 Xinling Road, Shantou, 515041 Guangdong, China. FAU - Wang, Ping AU - Wang P AD - Jinxin Research Institute for Reproductive Medicine and Genetics, 66 Bisheng Road, Chengdu, 610066 Sichuan, China. FAU - Chen, Jianying AU - Chen J AD - Jinxin Research Institute for Reproductive Medicine and Genetics, 66 Bisheng Road, Chengdu, 610066 Sichuan, China. FAU - Zeng, Liting AU - Zeng L AD - Department of Pathology and Provincial Key Laboratory of Infectious Diseases and Immunopathology, Collaborative and Creative Center, Shantou University Medical College, 22 Xinling Road, Shantou, 515041 Guangdong, China. FAU - Qu, Ting AU - Qu T AD - Jinxin Research Institute for Reproductive Medicine and Genetics, 66 Bisheng Road, Chengdu, 610066 Sichuan, China. FAU - Maldonado, Martin AU - Maldonado M AD - Jinxin Research Institute for Reproductive Medicine and Genetics, 66 Bisheng Road, Chengdu, 610066 Sichuan, China. FAU - Huang, Jihua AU - Huang J AD - Jinxin Research Institute for Reproductive Medicine and Genetics, 66 Bisheng Road, Chengdu, 610066 Sichuan, China. FAU - Han, Tingting AU - Han T AD - Jinxin Research Institute for Reproductive Medicine and Genetics, 66 Bisheng Road, Chengdu, 610066 Sichuan, China. FAU - Wen, Zina AU - Wen Z AD - Department of Andrology, Chengdu Xi'nan Gynecological Hospital, 66 Bisheng Road, Chengdu, 610066 Sichuan, China. FAU - Tian, Erpo AU - Tian E AD - Department of Andrology, Chengdu Xi'nan Gynecological Hospital, 66 Bisheng Road, Chengdu, 610066 Sichuan, China. FAU - Meng, Xiangqian AU - Meng X AD - Department of Embryology, Chengdu Jinjiang Hospital for Maternal and Child Health Care, 3 San-guantang Road, Chengdu, 610066 Sichuan, China. FAU - Zhong, Ying AU - Zhong Y AUID- ORCID: 0000-0001-9041-3172 AD - Department of Embryology, Chengdu Jinjiang Hospital for Maternal and Child Health Care, 3 San-guantang Road, Chengdu, 610066 Sichuan, China. FAU - Gu, Jiang AU - Gu J AUID- ORCID: 0000-0002-0706-8329 AD - Department of Pathology and Provincial Key Laboratory of Infectious Diseases and Immunopathology, Collaborative and Creative Center, Shantou University Medical College, 22 Xinling Road, Shantou, 515041 Guangdong, China. LA - eng PT - Journal Article DEP - 20220618 PL - United States TA - Stem Cells Int JT - Stem cells international JID - 101535822 PMC - PMC9233600 COIS- The authors declare no conflict of interest. EDAT- 2022/06/29 06:00 MHDA- 2022/06/29 06:01 PMCR- 2022/06/18 CRDT- 2022/06/28 01:54 PHST- 2022/01/19 00:00 [received] PHST- 2022/05/19 00:00 [revised] PHST- 2022/05/20 00:00 [accepted] PHST- 2022/06/28 01:54 [entrez] PHST- 2022/06/29 06:00 [pubmed] PHST- 2022/06/29 06:01 [medline] PHST- 2022/06/18 00:00 [pmc-release] AID - 10.1155/2022/7819234 [doi] PST - epublish SO - Stem Cells Int. 2022 Jun 18;2022:7819234. doi: 10.1155/2022/7819234. eCollection 2022.