PMID- 35762511
OWN - NLM
STAT- MEDLINE
DCOM- 20220629
LR  - 20220906
IS  - 1477-9137 (Electronic)
IS  - 0021-9533 (Linking)
VI  - 135
IP  - 12
DP  - 2022 Jun 15
TI  - Syntaxin 7 contributes to breast cancer cell invasion by promoting invadopodia 
      formation.
LID - jcs259576 [pii]
LID - 10.1242/jcs.259576 [doi]
AB  - Invasion in various cancer cells requires coordinated delivery of signaling 
      proteins, adhesion proteins, actin-remodeling proteins and proteases to 
      matrix-degrading structures called invadopodia. Vesicular trafficking involving 
      SNAREs plays a crucial role in the delivery of cargo to the target membrane. 
      Screening of 13 SNAREs from the endocytic and recycling route using a gene 
      silencing approach coupled with functional assays identified syntaxin 7 (STX7) as 
      an important player in MDA-MB-231 cell invasion. Total internal reflection 
      fluorescence microscopy (TIRF-M) studies revealed that STX7 resides near 
      invadopodia and co-traffics with MT1-MMP (also known as MMP14), indicating a 
      possible role for this SNARE in protease trafficking. STX7 depletion reduced the 
      number of invadopodia and their associated degradative activity. 
      Immunoprecipitation studies revealed that STX7 forms distinct SNARE complexes 
      with VAMP2, VAMP3, VAMP7, STX4 and SNAP23. Depletion of VAMP2, VAMP3 or STX4 
      abrogated invadopodia formation, phenocopying what was seen upon lack of STX7. 
      Whereas depletion of STX4 reduced MT1-MMP level at the cell surfaces, STX7 
      silencing significantly reduced the invadopodia-associated MT1-MMP pool and 
      increased the non-invadosomal pool. This study highlights STX7 as a major 
      contributor towards the invadopodia formation during cancer cell invasion. This 
      article has an associated First Person interview with the first author of the 
      paper.
CI  - (c) 2022. Published by The Company of Biologists Ltd.
FAU - Parveen, Sameena
AU  - Parveen S
AUID- ORCID: 0000-0002-9181-8283
AD  - Department of Biological Sciences, Indian Institute of Science Education and 
      Research, Bhopal, Bhopal 462066, India.
FAU - Khamari, Amrita
AU  - Khamari A
AUID- ORCID: 0000-0003-3454-2759
AD  - Department of Biological Sciences, Indian Institute of Science Education and 
      Research, Bhopal, Bhopal 462066, India.
FAU - Raju, Jyothikamala
AU  - Raju J
AUID- ORCID: 0000-0001-9444-6211
AD  - Thazhathemalayil House, Thodupuzha East PO, Keerikode, Kerala 685585, India.
FAU - Coppolino, Marc G
AU  - Coppolino MG
AUID- ORCID: 0000-0002-4538-7929
AD  - Department of Molecular and Cellular Biology, University of Guelph, Ontario N1G 
      2W1, Canada.
FAU - Datta, Sunando
AU  - Datta S
AUID- ORCID: 0000-0002-1417-0276
AD  - Department of Biological Sciences, Indian Institute of Science Education and 
      Research, Bhopal, Bhopal 462066, India.
LA  - eng
GR  - MHRD/BIO/2019047/Ministry of Human Resources and Development, India/
GR  - CRG/2019/004580/Science and Engineering Research Board/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220628
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Qa-SNARE Proteins)
RN  - 0 (SNARE Proteins)
RN  - 0 (Vesicle-Associated Membrane Protein 2)
RN  - 0 (Vesicle-Associated Membrane Protein 3)
RN  - EC 3.4.24.80 (Matrix Metalloproteinase 14)
SB  - IM
MH  - *Breast Neoplasms/genetics/metabolism
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinase 14/genetics/metabolism
MH  - Neoplasm Invasiveness
MH  - *Podosomes/metabolism
MH  - Protein Transport
MH  - *Qa-SNARE Proteins/genetics/metabolism
MH  - SNARE Proteins/metabolism
MH  - Vesicle-Associated Membrane Protein 2/genetics/metabolism
MH  - Vesicle-Associated Membrane Protein 3/metabolism
OTO - NOTNLM
OT  - Cortactin
OT  - Invadopodia
OT  - MT1-MMP
OT  - SNAREs
OT  - STX4
OT  - STX7
OT  - Tks5
OT  - VAMP2
OT  - VAMP3
COIS- Competing interests The authors declare no competing or financial interests.
EDAT- 2022/06/29 06:00
MHDA- 2022/06/30 06:00
CRDT- 2022/06/28 07:12
PHST- 2021/11/12 00:00 [received]
PHST- 2022/05/12 00:00 [accepted]
PHST- 2022/06/28 07:12 [entrez]
PHST- 2022/06/29 06:00 [pubmed]
PHST- 2022/06/30 06:00 [medline]
AID - 275829 [pii]
AID - 10.1242/jcs.259576 [doi]
PST - ppublish
SO  - J Cell Sci. 2022 Jun 15;135(12):jcs259576. doi: 10.1242/jcs.259576. Epub 2022 Jun 
      28.