PMID- 35764514
OWN - NLM
STAT- MEDLINE
DCOM- 20220630
LR  - 20220630
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 47
IP  - 6
DP  - 2022 Jun 25
TI  - [Electroacupuncture improves learning-memory ability by down-regulating 
      expression of hippocampal Abeta1-42 and Tau and NF-kappaB proteins in diabetic rats with 
      cognitive impairment].
PG  - 485-90
LID - 10.13702/j.1000-0607.20210707 [doi]
AB  - OBJECTIVE: To observe the effect of electroacupuncture(EA) on the expression of 
      nuclear transcription factors kappaB (NF-kappaB) and Tau protein and content of amyloid 
      (Abeta) in diabetic rats with cognitive impairment, so as to explore its mechanism 
      underlying improvement of learning-memory ability. METHODS: Male SD rats were 
      randomly divided into normal control (n=9), model (n=9) and EA (n=9) groups. The 
      diabetic mellitus model was established by feeding the rats with high fat and 
      high sugar for 1 month and intraperitoneal injection of STZ (25 mg.kg-1.d-1) for 
      2 days. EA was applied to "Zusanli"(ST36) and "Neiting"(ST44) for 20 min, 
      alternatively on both side every day, and "Yishu"(EX-B3) was stimulated by 
      twirling the acupuncture needle with uniform reinforcing-reducing method for 1 
      min, followed by retaining it for 20 min. The treatment was conducted 6 times a 
      week for 4 weeks. The learning-memory ability was evaluated by using Morris water 
      maze swimming test. The blood glucose level was randomly detected by using a 
      glucometer, the content of Abeta1-42 in the hippocampal tissue was detected by 
      ELISA, and the relative expression levels of hippocampal Tau and NF-kappaB p65 
      proteins and mRNAs were determined by Western blot and fluorescence quantitative 
      real-time PCR, separately. RESULTS: After modeling, the blood glucose, escape 
      latency, Abeta1-42 content and the expression levels of Tau and NF-kappaB p65 proteins 
      and mRNAs in the model group were significantly increased (P<0.01, P<0.05) in 
      comparison with the normal control group. Following EA intervention, the modeling 
      induced increase of blood glucose, escape latency, Abeta1-42 content and the 
      expression levels of Tau and NF-kappaB p65 proteins and mRNAs were reversed (P<0.05, 
      P<0.01). CONCLUSION: EA can improve the learning-memory ability in rats with 
      diabetic cognitive impairment, which may be related to its function in 
      down-regulating the levels of hippocampal Abeta1-42, Tau and NF-kappaB proteins.
FAU - He, Chao
AU  - He C
AD  - Graduate School of Anhui University of Chinese Medicine, Hefei 230038, China.
FAU - Yuan, Ai-Hong
AU  - Yuan AH
AD  - The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 
      230031.
FAU - Yang, Jun
AU  - Yang J
AD  - The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 
      230031.
FAU - Fan, Yin-Qiu
AU  - Fan YQ
AD  - The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 
      230031.
FAU - Xie, Hong-Yu
AU  - Xie HY
AD  - The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 
      230031.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (Amyloid beta-Peptides)
RN  - 0 (Blood Glucose)
RN  - 0 (NF-kappa B)
RN  - 0 (Peptide Fragments)
RN  - 0 (amyloid beta-protein (1-42))
RN  - 0 (tau Proteins)
SB  - IM
MH  - Amyloid beta-Peptides
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - *Cognitive Dysfunction/genetics/therapy
MH  - *Diabetes Mellitus, Experimental/genetics/metabolism/therapy
MH  - *Electroacupuncture
MH  - Hippocampus/metabolism
MH  - Male
MH  - Memory
MH  - NF-kappa B/genetics/metabolism
MH  - Peptide Fragments
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - tau Proteins/genetics/metabolism
OTO - NOTNLM
OT  - Abeta1-42
OT  - Cognitive impairment
OT  - Diabetes mellitus
OT  - Electroacupuncture
OT  - Hippocampus
OT  - Learning-memory ability
OT  - Nuclear transcription factors kappaB
OT  - Tau protein
EDAT- 2022/06/29 06:00
MHDA- 2022/07/01 06:00
CRDT- 2022/06/28 22:12
PHST- 2022/06/28 22:12 [entrez]
PHST- 2022/06/29 06:00 [pubmed]
PHST- 2022/07/01 06:00 [medline]
AID - 10.13702/j.1000-0607.20210707 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2022 Jun 25;47(6):485-90. doi: 10.13702/j.1000-0607.20210707.