PMID- 35765856 OWN - NLM STAT- MEDLINE DCOM- 20221012 LR - 20221104 IS - 1745-4514 (Electronic) IS - 0145-8884 (Linking) VI - 46 IP - 10 DP - 2022 Oct TI - Anti-diabetic effect of banana peel dietary fibers on type 2 diabetic mellitus mice induced by streptozotocin and high-sugar and high-fat diet. PG - e14275 LID - 10.1111/jfbc.14275 [doi] AB - We used a high-fat diet (HFD) and streptozotocin (STZ) to induce type 2 diabetic mellitus (T2DM) mice and evaluated the effect of banana peel dietary fibers (BP-DFs) as potential hypoglycemic agents. After 5 weeks of intervention with banana peel dietary fibers (BP-DFs), food intake was reduced, body weight was increased, blood lipids and glucose were reduced, fasting insulin and GLP-1 levels were increased, and liver and pancreatic tissue damage was reduced. Banana peel soluble dietary fiber (BP-SDF) has the most significant effect. The results of fecal microbiota analysis showed that BP-DFs could ameliorates gut microbiome dysbiosis, and all three types of dietary fibers have obvious effects. The results of fecal short-chain fatty acids (SCFAs) showed that the content of fecal SCFAs was increased after BP-DFs dietary intervention, and BP-SDF had the most obvious effect. RT-PCR experiment results show that BP-DFs can up-regulate the mRNA expression levels of PI3K, AKT, IRS-1, and FOXO1 in the liver of diabetic mice, which indicates that BD-DFs may play a role in improving insulin resistance and insulin signal transduction via the IRS/PI3K/AKT pathway, improving insulin resistance and insulin signal transduction. Our research may be extended to BP-DFs, especially BP-SDF, as the basis for potential dietary intervention to prevent or treat type 2 diabetic mellitus. This work supports future research studies of the anti-diabetic properties of BP-SDF in humans. PRACTICAL APPLICATIONS: Diabetes can lead to a variety of complications that have a huge impact on health. Dietary fiber may help in lowering blood sugar. Our experimental results showed that banana peel dietary fibers have the effect of reducing food intake, blood sugar, improving liver and pancreas function, increasing the abundance of intestinal flora, and improving the IRS/PI3K/AKT pathway in T2DM mice. Therefore, this study could provide a theoretical basis for the development of functional foods with banana peel dietary fiber. CI - (c) 2022 Wiley Periodicals LLC. FAU - Wang, Mengyang AU - Wang M AD - Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, Anhui, PR China. AD - School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, PR China. FAU - Yang, Fan AU - Yang F AD - Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, Anhui, PR China. AD - School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, PR China. FAU - Yan, Xiang AU - Yan X AD - Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, Anhui, PR China. AD - School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, PR China. FAU - Chao, Xinyu AU - Chao X AD - Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, Anhui, PR China. AD - School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, PR China. FAU - Zhang, Wencheng AU - Zhang W AD - Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, Anhui, PR China. AD - School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, PR China. FAU - Yuan, Chuanxun AU - Yuan C AD - Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, Anhui, PR China. AD - School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, PR China. FAU - Zeng, Qingmei AU - Zeng Q AUID- ORCID: 0000-0003-1711-9887 AD - Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei, Anhui, PR China. AD - School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui, PR China. LA - eng GR - 31071556/National Natural Science Foundation of China/ GR - 33171844/National Natural Science Foundation of China/ GR - 807094537012/the Science and Technology Department of Anhui province/ GR - 912268728082/the Science and Technology Department of Anhui province/ PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220629 PL - United States TA - J Food Biochem JT - Journal of food biochemistry JID - 7706045 RN - 0 (Blood Glucose) RN - 0 (Dietary Fiber) RN - 0 (Hypoglycemic Agents) RN - 0 (Insulin) RN - 0 (Lipids) RN - 0 (RNA, Messenger) RN - 5W494URQ81 (Streptozocin) RN - 89750-14-1 (Glucagon-Like Peptide 1) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) RN - IY9XDZ35W2 (Glucose) SB - IM MH - Animals MH - Blood Glucose MH - *Diabetes Mellitus, Experimental/drug therapy/metabolism MH - *Diabetes Mellitus, Type 2/drug therapy MH - Diet, High-Fat MH - Dietary Fiber MH - Glucagon-Like Peptide 1 MH - Glucose MH - Humans MH - Hypoglycemic Agents/pharmacology MH - Insulin MH - *Insulin Resistance MH - Lipids MH - Mice MH - *Musa/metabolism MH - Phosphatidylinositol 3-Kinases/genetics/metabolism MH - Proto-Oncogene Proteins c-akt/metabolism MH - RNA, Messenger MH - Streptozocin OTO - NOTNLM OT - SCFAs OT - banana peels OT - dietary fiber OT - gut microbiota OT - type 2 diabetes mellitus EDAT- 2022/06/30 06:00 MHDA- 2022/10/13 06:00 CRDT- 2022/06/29 03:32 PHST- 2022/04/16 00:00 [revised] PHST- 2022/01/19 00:00 [received] PHST- 2022/05/28 00:00 [accepted] PHST- 2022/06/30 06:00 [pubmed] PHST- 2022/10/13 06:00 [medline] PHST- 2022/06/29 03:32 [entrez] AID - 10.1111/jfbc.14275 [doi] PST - ppublish SO - J Food Biochem. 2022 Oct;46(10):e14275. doi: 10.1111/jfbc.14275. Epub 2022 Jun 29.