PMID- 35768837
OWN - NLM
STAT- MEDLINE
DCOM- 20220701
LR  - 20220716
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 18
IP  - 1
DP  - 2022 Jun 29
TI  - Long read sequencing revealed proventricular virome of broiler chicken with 
      transmission viral proventriculitis.
PG  - 253
LID - 10.1186/s12917-022-03339-9 [doi]
LID - 253
AB  - BACKGROUND: Transmissible viral proventriculitis (TVP) causes significant 
      economic loss to the poultry industry. However, the exact causative agents are 
      obscure. Here we examine the virome of proventriculus from specified pathogen 
      free (SPF) chickens that reproduced by infection of proventricular homogenate 
      from broiler chicken with TVP using long read sequencing of the Pacific 
      Biosciences RSII platform. The normal SPF chickens were used as control. RESULTS: 
      Our investigation reveals a virome of proventriculitis, including three Gyrovirus 
      genera of the Aneloviridae: Gyrovirus homsa1 (GyH1) (also known as Gyrovirus 3, 
      GyV3) (n = 2662), chicken anemia virus (CAV) (n = 482) and Gyrovirus galga1 
      (GyG1) (also known as avian Gyrovirus 2, AGV2) (n = 11); a plethora of novel 
      CRESS viral genomes (n = 26) and a novel genomovirus. The 27 novel viruses were 
      divided into three clusters. Phylogenetic analysis showed that the GyH1 strain 
      was more closely related to the strains from chicken (MG366592) than mammalian 
      (human and cat), the GyG1 strain was closely related to the strains from cat in 
      China (MK089245) and from chicken in Brazil (HM590588), and the CAV strain was 
      more closely related to the strains from Germany (AJ297684) and United Kingdom 
      (U66304) than that previously found in China. CONCLUSION: In this study, we 
      revealed that Gyrovirus virome showed high abundance in chickens with TVP, 
      suggesting their potential role in TVP, especially GyH1. This study is expected 
      to contribute to the knowledge of the etiology of TVP.
CI  - (c) 2022. The Author(s).
FAU - Yan, Tianxing
AU  - Yan T
AD  - Present Address: College of Veterinary Medicine, Shandong Agricultural 
      University, Shandong Provence, Tai'an, 271018, China.
FAU - Li, Gen
AU  - Li G
AD  - Present Address: College of Veterinary Medicine, Shandong Agricultural 
      University, Shandong Provence, Tai'an, 271018, China.
AD  - College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266000, 
      China.
FAU - Zhou, Defang
AU  - Zhou D
AD  - Present Address: College of Veterinary Medicine, Shandong Agricultural 
      University, Shandong Provence, Tai'an, 271018, China.
FAU - Hu, Liping
AU  - Hu L
AD  - Animal Epidemic Prevention and Control Center of Shandong Province, Jinan, China.
FAU - Hao, Xiaojing
AU  - Hao X
AD  - Animal Husbandry and Veterinary Research Institute of Qingdao, Qingdao, China.
FAU - Li, Ruiqi
AU  - Li R
AD  - Present Address: College of Veterinary Medicine, Shandong Agricultural 
      University, Shandong Provence, Tai'an, 271018, China.
FAU - Wang, Guihua
AU  - Wang G
AD  - Present Address: College of Veterinary Medicine, Shandong Agricultural 
      University, Shandong Provence, Tai'an, 271018, China.
FAU - Cheng, Ziqiang
AU  - Cheng Z
AD  - Present Address: College of Veterinary Medicine, Shandong Agricultural 
      University, Shandong Provence, Tai'an, 271018, China. czqsd@126.com.
LA  - eng
GR  - SDAIT-11-04/Modern Agricultural Technology Industry System of Shandong province/
GR  - SDAIT-11-04/Modern Agricultural Technology Industry System of Shandong province/
GR  - SDAIT-11-04/Modern Agricultural Technology Industry System of Shandong province/
GR  - SDAIT-11-04/Modern Agricultural Technology Industry System of Shandong province/
GR  - 2019JZZY010735/Shandong Science and Technology Development Project/
GR  - 2019JZZY010735/Shandong Science and Technology Development Project/
GR  - 2019JZZY010735/Shandong Science and Technology Development Project/
GR  - 2019JZZY010735/Shandong Science and Technology Development Project/
PT  - Journal Article
DEP - 20220629
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
SB  - IM
MH  - Animals
MH  - *Chicken anemia virus/genetics
MH  - Chickens
MH  - *Gyrovirus
MH  - Mammals
MH  - Phylogeny
MH  - *Poultry Diseases
MH  - Proventriculus
MH  - *Stomach Diseases/veterinary
MH  - Virome
PMC - PMC9241223
OTO - NOTNLM
OT  - Gyrovirus
OT  - Novel CRESS virus
OT  - Pacific Biosciences RSII platform
OT  - Transmissible viral proventriculitis
OT  - Virome
COIS- There are no competing interests in the study.
EDAT- 2022/06/30 06:00
MHDA- 2022/07/02 06:00
PMCR- 2022/06/29
CRDT- 2022/06/29 23:42
PHST- 2021/12/14 00:00 [received]
PHST- 2022/06/07 00:00 [accepted]
PHST- 2022/06/29 23:42 [entrez]
PHST- 2022/06/30 06:00 [pubmed]
PHST- 2022/07/02 06:00 [medline]
PHST- 2022/06/29 00:00 [pmc-release]
AID - 10.1186/s12917-022-03339-9 [pii]
AID - 3339 [pii]
AID - 10.1186/s12917-022-03339-9 [doi]
PST - epublish
SO  - BMC Vet Res. 2022 Jun 29;18(1):253. doi: 10.1186/s12917-022-03339-9.