PMID- 35769615
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2671-8790 (Print)
IS  - 2671-8804 (Electronic)
IS  - 2671-8790 (Linking)
VI  - 35
IP  - 1
DP  - 2021 Mar 31
TI  - Comparison of single nucleotide polymorphisms and short tandem repeats as markers 
      for differentiating between donors and recipients in solid organ transplantation.
PG  - 1-7
LID - 10.4285/kjt.20.0035 [doi]
AB  - BACKGROUND: To analyze transplant rejection and to distinguish between donor and 
      recipient, it is necessary to select a marker from single nucleotide polymorphism 
      (SNP), short tandem repeat (STR), and human leukocyte antigen (HLA) testing. SNPs 
      are bi-allelic and the polymerase chain reaction method used for SNP testing has 
      the advantage of lower cost than sequencing methods. In this study, we aimed to 
      distinguish donors from recipients using a combination of existing commercialized 
      STRs and the SNPs identified. METHODS: All selected SNPs complied with the 
      following criterion known and validated minor allele frequency (MAF) >/=43% in 
      Korean and reported ethnicities from global populations (HapMap, 1000 Genomes, 
      and the Korean Reference Genome project). The STR assays were performed for 16 
      tetranucleotide repeat loci. RESULTS: DNA from the 52 donor/recipient pairs were 
      tested for informative markers. The median age of the recipients was 47 years. 
      MAF in the 52 pairs was 1.0%-76.0%. The probability of informative genotypes (I) 
      was 0.001-0.124. The summation of I was 0.680. In the 52 donor recipient pairs, 
      the selected SNPs showed a 0.031 average probability of being informative. The 
      probability of identity in our study was 0.122-0.348. SNP panel configuration 
      distinguished 100% of 52 donors/recipient pairs. CONCLUSIONS: Donors and 
      recipients were distinguished by STR and 22 SNPs with MAF identified from SNP 
      databases. Seventeen SNPs were able to distinguish between donors and recipients 
      (I value=0.039).
CI  - Copyright (c) 2021 The Korean Society for Transplantation.
FAU - Lee, Hyun-Ji
AU  - Lee HJ
AUID- ORCID: 0000-0002-9021-5632
AD  - Department of Laboratory Medicine, and Research Institute for Convergence of 
      Biomedical Science and Technology, Pusan National University Yangsan Hospital, 
      Yangsan, Korea.
FAU - Shin, Kyoung-Hwa
AU  - Shin KH
AUID- ORCID: 0000-0002-8454-4448
AD  - Department of Laboratory Medicine, Pusan National University Hospital, Busan, 
      Korea.
FAU - Jeong, Su Jeong
AU  - Jeong SJ
AUID- ORCID: 0000-0002-5581-3813
AD  - Department of Laboratory Medicine, and Research Institute for Convergence of 
      Biomedical Science and Technology, Pusan National University Yangsan Hospital, 
      Yangsan, Korea.
FAU - Kim, In Suk
AU  - Kim IS
AUID- ORCID: 0000-0002-7243-9173
AD  - Department of Laboratory Medicine, and Research Institute for Convergence of 
      Biomedical Science and Technology, Pusan National University Yangsan Hospital, 
      Yangsan, Korea.
LA  - eng
PT  - Journal Article
DEP - 20210224
PL  - Korea (South)
TA  - Korean J Transplant
JT  - Korean journal of transplantation
JID - 101775609
PMC - PMC9235337
OTO - NOTNLM
OT  - Chimerism
OT  - Organ transplantation
OT  - Short tandem repeats
OT  - Single nucleotide polymorphism
EDAT- 2021/03/31 00:00
MHDA- 2021/03/31 00:01
PMCR- 2021/03/31
CRDT- 2022/06/30 02:40
PHST- 2020/08/14 00:00 [received]
PHST- 2020/11/12 00:00 [revised]
PHST- 2020/12/10 00:00 [accepted]
PHST- 2022/06/30 02:40 [entrez]
PHST- 2021/03/31 00:00 [pubmed]
PHST- 2021/03/31 00:01 [medline]
PHST- 2021/03/31 00:00 [pmc-release]
AID - kjt-35-1-1 [pii]
AID - 10.4285/kjt.20.0035 [doi]
PST - ppublish
SO  - Korean J Transplant. 2021 Mar 31;35(1):1-7. doi: 10.4285/kjt.20.0035. Epub 2021 
      Feb 24.