PMID- 35770634
OWN - NLM
STAT- MEDLINE
DCOM- 20220701
LR  - 20220701
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 74
IP  - 3
DP  - 2022 Jun 25
TI  - [Noradrenaline modulates the spontaneous firing activities of Purkinje cells via 
      alpha2-adrenergic receptor in mouse cerebellar cortex].
PG  - 359-369
AB  - Cerebellar Purkinje cells (PCs) exhibit two types of discharge activities: simple 
      spike (SS) and complex spike (CS). Previous studies found that noradrenaline (NA) 
      can inhibit CS and bidirectionally regulate SS, but the enhancement of NA on SS 
      is overwhelmed by the strong inhibition of excitatory molecular layer 
      interneurons. However, the mechanism underlying the effect of NA on SS discharge 
      frequency is not clear. Therefore, in the present study, we examined the 
      mechanism underlying the increasing effect of NA on SS firing of PC in mouse 
      cerebellar cortex in vivo and in cerebellar slice by cell-attached and whole-cell 
      recording technique and pharmacological methods. GABA(A) receptor was blocked by 
      100 micromol/L picrotoxin in the whole process. In vivo results showed that NA 
      significantly reduced the number of spikelets of spontaneous CS and enhanced the 
      discharge frequency of SS, but did not affect the discharge frequency of CS. In 
      vitro experiments showed that NA reduced the number of CS spikelets and after 
      hyperpolarization potential (AHP) induced by electrical stimulation, and 
      increased the discharge frequency of SS. NA also reduced the amplitude of 
      excitatory postsynaptic current (EPSC) of parallel fiber (PF)-PC and 
      significantly increased the paired-pulse ratio (PPR). Application of yohimbine, 
      an antagonist of alpha2-adrenergic receptor (AR), completely eliminated the enhancing 
      effect of NA on SS. The alpha2-AR agonist, UK14304, also increased the frequency of 
      SS. The beta-AR blocker, propranolol, did not affect the effects of NA on PC. These 
      results suggest that in the absence of GABA(A) receptors, NA could attenuate the 
      synaptic transmission of climbing fiber (CF)-PC via activating alpha2-AR, inhibit CS 
      activity and reduce AHP, thus enhancing the SS discharge frequency of PC. This 
      result suggests that NA neurons of locus coeruleus can finely regulate PC signal 
      output by regulating CF-PC synaptic transmission.
FAU - Zhang, Xu-Dong
AU  - Zhang XD
AD  - Brain Science Research Center of Yanbian University, Yanji 133002, China.
AD  - Department of Physiology and Pathophysiology, College of Medicine, Yanbian 
      University, Yanji 133002, China.
FAU - Wang, Li-Fei
AU  - Wang LF
AD  - Department of Basic Medicine, College of Medicine, Changchun Sci-Tech University, 
      Changchun 130600, China.
FAU - Xuan, Fang-Ling
AU  - Xuan FL
AD  - Brain Science Research Center of Yanbian University, Yanji 133002, China.
AD  - Department of Physiology and Pathophysiology, College of Medicine, Yanbian 
      University, Yanji 133002, China.
FAU - Qiu, De-Lai
AU  - Qiu DL
AD  - Brain Science Research Center of Yanbian University, Yanji 133002, China.
AD  - Department of Physiology and Pathophysiology, College of Medicine, Yanbian 
      University, Yanji 133002, China.
FAU - Zhang, Bin-Bin
AU  - Zhang BB
AD  - Brain Science Research Center of Yanbian University, Yanji 133002, China.
AD  - Department of Physiology and Pathophysiology, College of Medicine, Yanbian 
      University, Yanji 133002, China. bbZhang@ybu.edu.cn.
FAU - Chu, Chun-Ping
AU  - Chu CP
AD  - Brain Science Research Center of Yanbian University, Yanji 133002, China.
AD  - Department of Physiology and Pathophysiology, College of Medicine, Yanbian 
      University, Yanji 133002, China. cpchu@ybu.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (Receptors, Adrenergic, alpha-2)
RN  - 0 (Receptors, GABA-A)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Action Potentials/physiology
MH  - Animals
MH  - Cerebellar Cortex/metabolism
MH  - Cerebellum/metabolism
MH  - Mice
MH  - *Norepinephrine/pharmacology
MH  - *Purkinje Cells/metabolism
MH  - Receptors, Adrenergic, alpha-2/metabolism
MH  - Receptors, GABA-A/metabolism
EDAT- 2022/07/01 06:00
MHDA- 2022/07/02 06:00
CRDT- 2022/06/30 05:03
PHST- 2022/06/30 05:03 [entrez]
PHST- 2022/07/01 06:00 [pubmed]
PHST- 2022/07/02 06:00 [medline]
PST - ppublish
SO  - Sheng Li Xue Bao. 2022 Jun 25;74(3):359-369.