PMID- 35773566
OWN - NLM
STAT- MEDLINE
DCOM- 20220704
LR  - 20220711
IS  - 1432-072X (Electronic)
IS  - 0302-8933 (Linking)
VI  - 204
IP  - 7
DP  - 2022 Jun 30
TI  - Modulatory apoptotic effects of sinomenine on Mycoplasma pneumonia through the 
      attenuation of inflammation via ERK/JNK/NF-kappaB signaling pathway.
PG  - 441
LID - 10.1007/s00203-022-03039-w [doi]
AB  - Mycoplasma pneumoniae (MPP) induced pneumonia is a common disease of children. 
      Sinomenine (SIN) is an isoquinoline mainly sequestered from Sinomenium acutum. It 
      is a promising drug for treating arthritis, lung, colon, liver and gastric 
      cancer. Hence, the present study investigated the role and mechanism of SIN 
      treatment in MPP induced pneumonia in experimental in-vivo mice model. The BALB/c 
      male mice were separated into four groups (n = 6 mice/group): normal, MPP, 
      MPP + SIN (20 mg/kg bw), and SIN (20 mg/kg bw) alone. Results were expressed as 
      mean +/- SD. Data were analyzed using one way Analysis of Variance (ANOVA) with the 
      Dunnett's post hoc test using SPSS v 18.0. P value < 0.05 was considered 
      significant. The total protein, cell count, inflammatory cytokines, MP-IgM, 
      Monocyte chemo attractant protein-1 (MCP-1), and MP-DNA were measured. The 
      protein expressions of Bax/Bcl-2, ERK, JNK, NF-kappaB were analyzed and 
      histopathology of lungs was examined. SIN treatment significantly (p < 0.05) 
      reduced the total proteins, cell counts in BALF, inflammatory cytokines, MP-IgM, 
      MCP-1, MP-DNA and reversed the histological alterations. SIN attenuated the 
      apoptotic pathway through the modulation of Bax/Bcl-2 expression. SIN alleviated 
      pulmonary inflammatory mediators and apoptosis in MPP-infected mice via 
      suppression of ERK/JNK/NF-kappaB signaling. SIN administration diminished 
      inflammation and lung fibrosis by inhibiting apoptosis in MPP mice. Hence, SIN is 
      a potential natural protective remedy for MPP.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Chen, Yao
AU  - Chen Y
AD  - Department of Respiratory, Xi'an Children's Hospital, Xi'an City, 710000, China.
FAU - Zhang, Wen
AU  - Zhang W
AD  - Department of Respiratory, Xi'an Children's Hospital, Xi'an City, 710000, China.
FAU - Xin, Lihong
AU  - Xin L
AD  - Department of Respiratory, Xi'an Children's Hospital, Xi'an City, 710000, China.
FAU - Wang, Zhen
AU  - Wang Z
AD  - Department of Respiratory, Xi'an Children's Hospital, Xi'an City, 710000, China.
FAU - Zheng, Mao
AU  - Zheng M
AUID- ORCID: 0000-0002-2436-8956
AD  - Department of Emergency, Xi'an Children's Hospital, Xi'an City, 710000, China. 
      zhengmao19810908@sina.com.
FAU - Vijayalakshmi, Annamalai
AU  - Vijayalakshmi A
AD  - Department of Biochemistry, Rabiammal Ahamed Maideen College for Women, 
      Thiruvarur, Tamil Nadu, 610001, India.
LA  - eng
PT  - Journal Article
DEP - 20220630
PL  - Germany
TA  - Arch Microbiol
JT  - Archives of microbiology
JID - 0410427
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Morphinans)
RN  - 0 (NF-kappa B)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 63LT81K70N (sinomenine)
SB  - IM
MH  - Animals
MH  - Cytokines/metabolism
MH  - Immunoglobulin M
MH  - Inflammation
MH  - *MAP Kinase Signaling System/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Morphinans/pharmacology
MH  - *Mycoplasma pneumoniae/drug effects
MH  - *NF-kappa B/metabolism
MH  - *Pneumonia, Mycoplasma/drug therapy/metabolism
MH  - bcl-2-Associated X Protein/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Mycoplasma pneumonia
OT  - NF-kappaB
OT  - Sinomenine
OT  - cytokines
OT  - inflammation
EDAT- 2022/07/01 06:00
MHDA- 2022/07/06 06:00
CRDT- 2022/06/30 23:34
PHST- 2022/02/01 00:00 [received]
PHST- 2022/06/01 00:00 [accepted]
PHST- 2022/06/30 23:34 [entrez]
PHST- 2022/07/01 06:00 [pubmed]
PHST- 2022/07/06 06:00 [medline]
AID - 10.1007/s00203-022-03039-w [pii]
AID - 10.1007/s00203-022-03039-w [doi]
PST - epublish
SO  - Arch Microbiol. 2022 Jun 30;204(7):441. doi: 10.1007/s00203-022-03039-w.