PMID- 35774063
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2296-4185 (Print)
IS  - 2296-4185 (Electronic)
IS  - 2296-4185 (Linking)
VI  - 10
DP  - 2022
TI  - Stem Cells-Loaded 3D-Printed Scaffolds for the Reconstruction of Alveolar Cleft.
PG  - 939199
LID - 10.3389/fbioe.2022.939199 [doi]
LID - 939199
AB  - The advances in the field of tissue engineering and regenerative medicine have 
      opened new vistas for the repair of alveolar clefts. However, the currently 
      available biomaterials used for the repair of alveolar clefts have poor 
      mechanical properties and biocompatibility, which hinders the treatment outcomes. 
      Here, we aimed to develop 3D printed biomimetic scaffolds that fuses beta-tricalcium 
      phosphate (beta-TCP) and bone marrow mesenchymal stem cells (BMSCs) for improving 
      the repair of alveolar clefts. The methacrylate gelatin (GelMA) was mixed with 
      beta-TCP for the preparation of GelMA/beta-TCP hybrid scaffolds via 3D printing 
      platform and chemically cross-linking with UV light. The physicochemical 
      properties of the hydrogel scaffolds were characterized. Moreover, the survival 
      state, proliferation ability, morphological characteristics, and osteogenic 
      induction of BMSCs were examined. The prepared hybrid scaffolds showed good 
      biocompatibility and mechanical properties. BMSCs attached well to the scaffolds 
      and proliferated, survived, differentiated, and stimulated osteogenesis for the 
      reconstruction of alveolar clefts. We expect that use of the prepared hybrid 
      hydrogel scaffold can improve the outcomes of alveolar cleft repair in clinic and 
      expand the application of hybrid hydrogel in tissue engineering repair.
CI  - Copyright (c) 2022 Luo, Chen and Chen.
FAU - Luo, Dongyuan
AU  - Luo D
AD  - Guangzhou Women and Children's Medical Center, Guangzhou, China.
FAU - Chen, Boying
AU  - Chen B
AD  - Guangzhou Women and Children's Medical Center, Guangzhou, China.
FAU - Chen, Yu
AU  - Chen Y
AD  - Guangzhou Women and Children's Medical Center, Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20220614
PL  - Switzerland
TA  - Front Bioeng Biotechnol
JT  - Frontiers in bioengineering and biotechnology
JID - 101632513
PMC - PMC9237206
OTO - NOTNLM
OT  - 3D printing
OT  - alveolar cleft
OT  - bone marrow mesenchymal stem cells
OT  - methacrylated gelatin
OT  - beta-tricalcium phosphate
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/02 06:00
MHDA- 2022/07/02 06:01
PMCR- 2022/01/01
CRDT- 2022/07/01 02:25
PHST- 2022/05/08 00:00 [received]
PHST- 2022/05/26 00:00 [accepted]
PHST- 2022/07/01 02:25 [entrez]
PHST- 2022/07/02 06:00 [pubmed]
PHST- 2022/07/02 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 939199 [pii]
AID - 10.3389/fbioe.2022.939199 [doi]
PST - epublish
SO  - Front Bioeng Biotechnol. 2022 Jun 14;10:939199. doi: 10.3389/fbioe.2022.939199. 
      eCollection 2022.