PMID- 35774978
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 9
DP  - 2022
TI  - Detection of Multiple Lineages of PRRSV in Breeding and Growing Swine Farms.
PG  - 884733
LID - 10.3389/fvets.2022.884733 [doi]
LID - 884733
AB  - The detection and co-circulation of multiple variants of porcine reproductive and 
      respiratory syndrome virus (PRRSV) have been observed and reported in swine. 
      However, the potential long-term impact of multiple prevailing PRRSV variants on 
      pig-performance is not yet fully understood. The primary objective of this study 
      was to describe the genetic variation of PRRSV in processing fluid (PF), oral 
      fluid (OF), and tonsil scraping (TS) specimens from five swine farms with 
      different production types and PRRS status over a period of time (~1 year). 
      Furthermore, the association between PRRSV prevalence and production parameters 
      was investigated. Results showed that PRRSV was detected by RT-qPCR in 21-25% of 
      all types of specimens. In breeding farms, PRRSV detection in PF and/or TS 
      samples was correlated with stillborn and mummified fetuses, and pre-weaning 
      mortality throughout the study period. Although ORF5 sequences were obtained in 
      <16% of all sample types, simultaneous detection of PRRSV variants including 
      field and vaccine strains within a single sampling event was identified in both 
      breeding and growing pig farms. Phylogenetic analyses based on the ORF5 sequence 
      classified the detected field PRRSV into L1A and L1H, two sub-lineages of lineage 
      1 (L1). Our study demonstrated the presence of multiple PRRSV lineages, 
      sub-lineages, and variants in swine herds and its potential association with 
      swine reproductive performance under field conditions.
CI  - Copyright (c) 2022 Cheng, Campler, Schroeder, Yang, Mor, Ferreira and Arruda.
FAU - Cheng, Ting-Yu
AU  - Cheng TY
AD  - Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The 
      Ohio State University, Columbus, OH, United States.
FAU - Campler, Magnus R
AU  - Campler MR
AD  - Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The 
      Ohio State University, Columbus, OH, United States.
FAU - Schroeder, Declan C
AU  - Schroeder DC
AD  - Department of Veterinary Population Medicine, College of Veterinary Medicine, 
      University of Minnesota, Saint Paul, MN, United States.
AD  - School of Biological Sciences, University of Reading, Reading, United Kingdom.
FAU - Yang, My
AU  - Yang M
AD  - Department of Veterinary Population Medicine, College of Veterinary Medicine, 
      University of Minnesota, Saint Paul, MN, United States.
FAU - Mor, Sunil K
AU  - Mor SK
AD  - Department of Veterinary Population Medicine, College of Veterinary Medicine, 
      University of Minnesota, Saint Paul, MN, United States.
FAU - Ferreira, Juliana B
AU  - Ferreira JB
AD  - Department of Population Health & Pathobiology, College of Veterinary Medicine, 
      North Carolina State University, Raleigh, NC, United States.
FAU - Arruda, Andreia G
AU  - Arruda AG
AD  - Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The 
      Ohio State University, Columbus, OH, United States.
LA  - eng
PT  - Journal Article
DEP - 20220614
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC9237545
OTO - NOTNLM
OT  - oral fluid
OT  - porcine reproductive and respiratory syndrome virus
OT  - processing fluid
OT  - swine
OT  - tonsil scrapings
OT  - viral lineage
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/07/02 06:00
MHDA- 2022/07/02 06:01
PMCR- 2022/01/01
CRDT- 2022/07/01 02:46
PHST- 2022/02/26 00:00 [received]
PHST- 2022/05/11 00:00 [accepted]
PHST- 2022/07/01 02:46 [entrez]
PHST- 2022/07/02 06:00 [pubmed]
PHST- 2022/07/02 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2022.884733 [doi]
PST - epublish
SO  - Front Vet Sci. 2022 Jun 14;9:884733. doi: 10.3389/fvets.2022.884733. eCollection 
      2022.