PMID- 35775473
OWN - NLM
STAT- MEDLINE
DCOM- 20220928
LR  - 20220928
IS  - 1097-0029 (Electronic)
IS  - 1059-910X (Linking)
VI  - 85
IP  - 10
DP  - 2022 Oct
TI  - Screening of biosynthesized zinc oxide nanoparticles for their effect on Daucus 
      carota pathogen and molecular docking.
PG  - 3365-3373
LID - 10.1002/jemt.24191 [doi]
AB  - Herein, we investigate the phytogenic synthesis of zinc oxide nanoparticles 
      (ZnO-NPs) by using aqueous extract of seed coat of almond as a novel resource 
      which can acts as a stabilizing and reducing agents. Successful biosynthesis of 
      ZnO-NPs was observed by Ultraviolet-visible spectroscopy (UV-vis) showing peak at 
      ~272 nm. The scanning electron microscopy (SEM) and transmission electron 
      microscopy (TEM) techniques confirm the circular shape with an average size of 
      ~20 nm. Applications of ZnO-NPs were observed on carrot (Daucus carota) plant 
      infected with pathogenic fungus Rhizoctonia solani. Spray with 50 ppm and 100 ppm 
      ZnO-NPs caused significant increase in plant growth attributes and photosynthetic 
      pigments of carrot plants. It has been reported that the synthesized ZnO-NPs 
      demonstrated an inhibitory activity against plant pathogenic fungus R. solani and 
      reduces disease in carrot plants. Scanning electron microscopy and confocal 
      microscopy indicated adverse effect of ZnO-NPs on pathogens. Antifungal 
      efficiency of ZnO-NPs was further explained with help of molecular docking 
      analysis. Conformation with highest negative binding energy was used to predict 
      binding site of receptor with NPs to know mechanistic approach. ZnO-NPs are 
      likely to interact with the pathogens by mechanical enfolding which may be one of 
      the major toxicity actions against R. solani by ZnO-NPs. RESEARCH HIGHLIGHTS: ZnO 
      nanoparticles were synthesized using waste material from the coat of almond 
      seeds. Images from SEM, TEM, and related techniques like EDS and SAED revealed 
      the irregularity of the ZnO NPs as well as their atom composition. FTIR and XRD 
      analyses confirmed the formation and the presence of crystalline ZnO NPs in 
      nature. Biogenic ZnONPs were found to be effective against the plant pathogenic 
      fungus R. solani. A spray of 50 ppm and 100 ppm ZnO-NPs significantly increased 
      carrot plant growth characteristics and photosynthetic pigments.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - Khan, Azhar U
AU  - Khan AU
AD  - School of life and Basic Sciences, Department of Chemistry, SIILAS CAMPUS, Jaipur 
      National University, Jaipur, India.
FAU - Khan, Masudulla
AU  - Khan M
AD  - Botany Section, Women's College, Aligarh Muslim University, Aligarh, India.
FAU - Malik, Nazia
AU  - Malik N
AD  - Department of Chemistry, Aligarh Muslim University, Aligarh, India.
FAU - Parveen, Aiman
AU  - Parveen A
AD  - Department of Botany, Aligarh Muslim University, Aligarh, India.
FAU - Sharma, Poonam
AU  - Sharma P
AD  - School of life and Basic Sciences, Department of Chemistry, SIILAS CAMPUS, Jaipur 
      National University, Jaipur, India.
FAU - Min, Kim
AU  - Min K
AD  - Department of Safety Engineering, Dongguk University, 123 Dongdae-ro, 
      Gyeongju-si, Gyeongsangbuk-do, South Korea.
FAU - Gupta, Mona
AU  - Gupta M
AD  - Maharaja Bhoj Government PG College, Dhar, India.
FAU - Alam, Mahboob
AU  - Alam M
AUID- ORCID: 0000-0002-5188-1130
AD  - Department of Safety Engineering, Dongguk University, 123 Dongdae-ro, 
      Gyeongju-si, Gyeongsangbuk-do, South Korea.
LA  - eng
PT  - Journal Article
DEP - 20220701
PL  - United States
TA  - Microsc Res Tech
JT  - Microscopy research and technique
JID - 9203012
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Antifungal Agents)
RN  - 0 (Plant Extracts)
RN  - 0 (Reducing Agents)
RN  - SOI2LOH54Z (Zinc Oxide)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Antifungal Agents/pharmacology
MH  - *Daucus carota/metabolism
MH  - *Metal Nanoparticles/chemistry
MH  - Microbial Sensitivity Tests
MH  - Molecular Docking Simulation
MH  - Plant Extracts/chemistry
MH  - Reducing Agents
MH  - Spectroscopy, Fourier Transform Infrared
MH  - X-Ray Diffraction
MH  - *Zinc Oxide/chemistry/pharmacology
OTO - NOTNLM
OT  - Rhizoctonia solani
OT  - ZnO-NPs
OT  - almond
OT  - carrot
OT  - docking
EDAT- 2022/07/02 06:00
MHDA- 2022/09/28 06:00
CRDT- 2022/07/01 05:53
PHST- 2022/04/14 00:00 [revised]
PHST- 2022/01/27 00:00 [received]
PHST- 2022/06/19 00:00 [accepted]
PHST- 2022/07/02 06:00 [pubmed]
PHST- 2022/09/28 06:00 [medline]
PHST- 2022/07/01 05:53 [entrez]
AID - 10.1002/jemt.24191 [doi]
PST - ppublish
SO  - Microsc Res Tech. 2022 Oct;85(10):3365-3373. doi: 10.1002/jemt.24191. Epub 2022 
      Jul 1.