PMID- 35775675
OWN - NLM
STAT- MEDLINE
DCOM- 20220704
LR  - 20230913
IS  - 1307-7945 (Electronic)
IS  - 1306-696X (Print)
IS  - 1306-696X (Linking)
VI  - 28
IP  - 7
DP  - 2022 Jul
TI  - Effects of Carvedilol on liver ischemia-reperfusion injury in rats.
PG  - 885-893
LID - 10.14744/tjtes.2021.57824 [doi]
AB  - BACKGROUND: The aim of this study was to analyze the potential protective effect 
      of Carvedilol against liver ischemia-reperfusion (I/R) injury in rats. METHODS: A 
      total of 40 Wistar albino rats were randomly divided into four groups (n=10 
      each). Group I (Sham/Control group) underwent only laparotomy, Group II 
      (Carvedilol group) was administered carvedilol and then underwent laparotomy, 
      Group III (I/R group) underwent laparotomy and hepatic ischemia/reperfusion, and 
      Group IV (I/R + Carvedilol group) was administered carvedilol and then underwent 
      laparotomy and hepatic ischemia/reperfusion. Blood samples were collected for 
      malondialdehyde, glutathione (GSH), and myeloperoxidase (MPO) analysis. Liver 
      sections were obtained for histopathological analysis and stained with 
      hematoxy-lin-eosin. Tumor necrosis factor-alpha (TNF-alpha) and Caspase-3 primary 
      antibodies were used for the immunohistochemical analysis. RESULTS: Serum GSH 
      levels increased in the I/R + Carvedilol group. MPO activity was increased 
      signi fi cantly in the IR group. In I/R + Carvedilol group, serum MPO levels were 
      similar to the control group. Histopathological  fi ndings showed reduced dilatation 
      and congestion in vena centralis, regenerative changes in hepatocyte cells with 
      the protected nucleus structure in the I/R + Carvedilol group. Hepatocyte nuclei 
      with increased pycnosis and apoptosis and the dilated vena centralis were 
      observed in I/R group. In the control group, TNF-alpha showed a positive reaction in 
      macrophage cells around vena centralis. An increase in TNF-alpha expression was 
      observed in hepatocyte cells of I/R group. Positive expression of caspase-3 in 
      hepatocyte cells and a small number of endothelial and Kupffer cells were seen in 
      I/R group. However, negative caspase-3 expression was seen in hepatocyte, 
      endothelial, and Kupffer cells in I/R + Carvedilol group. CONCLUSION: Carvedilol 
      may prevent initiation of oxidative stress process, inflammation induction and 
      apoptotic progression.
FAU - Kafadar, Mehmet Tolga
AU  - Kafadar MT
AD  - Department of General Surgery, Dicle University Faculty of Medicine, 
      Diyarbakir-Turkiye.
FAU - Gok, Mehmet Ali
AU  - Gok MA
AD  - Department of General Surgery, Health Sciences University Kartal Dr. Lutfi Kirdar 
      City Hospital, Istanbul-Turkiye.
LA  - eng
PT  - Journal Article
TT  - Karvedilol'un sicanlarda karaciger iskemi-reperfuzyon hasari uzerine etkileri.
PL  - Turkey
TA  - Ulus Travma Acil Cerrahi Derg
JT  - Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency 
      surgery : TJTES
JID - 101274231
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0K47UL67F2 (Carvedilol)
RN  - EC 3.4.22.- (Caspase 3)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - *Carvedilol/pharmacology
MH  - Caspase 3/metabolism
MH  - Glutathione/metabolism
MH  - *Liver/blood supply/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - *Reperfusion Injury/drug therapy/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
PMC - PMC10493845
COIS- Conflict of Interest: None declared.
EDAT- 2022/07/02 06:00
MHDA- 2022/07/06 06:00
PMCR- 2022/07/01
CRDT- 2022/07/01 07:13
PHST- 2022/07/01 07:13 [entrez]
PHST- 2022/07/02 06:00 [pubmed]
PHST- 2022/07/06 06:00 [medline]
PHST- 2022/07/01 00:00 [pmc-release]
AID - TJTES-28-885 [pii]
AID - 10.14744/tjtes.2021.57824 [doi]
PST - ppublish
SO  - Ulus Travma Acil Cerrahi Derg. 2022 Jul;28(7):885-893. doi: 
      10.14744/tjtes.2021.57824.