PMID- 35776292
OWN - NLM
STAT- MEDLINE
DCOM- 20220706
LR  - 20220719
IS  - 1993-0402 (Electronic)
IS  - 1672-0415 (Linking)
VI  - 28
IP  - 7
DP  - 2022 Jul
TI  - Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating 
      Th1/Th2 Balance in DNCB-Induced BALB/c Mice.
PG  - 612-619
LID - 10.1007/s11655-022-3579-7 [doi]
AB  - OBJECTIVE: To evaluate the therapeutic effects of acupoint autohemotherapy 
      (A-AHT) on 1-chloro-2,4-dinitrobenzene (DNCB)-induced atopic dermatitis (AD) in 
      mice focusing on regulating T helper 1/T helper 2 (Th1/Th2) immune responses. 
      METHODS: Thirty BALB/c mice were divided into 5 groups by a random number table, 
      including normal control (NC), AD model (AD), A-AHT, sham A-AHT (sA-AHT), and 
      acupoint injection of normal saline (A-NS) groups, 6 mice per group. Mice were 
      challenged by DNCB for the establishment of experimental AD model. On the 8th 
      day, except for the NC and AD groups, the mice in the other groups received 
      management once every other day for a total of 28 days. For the A-AHT and sA-AHT 
      groups, 0.05 mL of autologous whole blood (AWB) was injected into bilateral 
      Zusanli (ST 36) and Quchi (LI 11) and sham-acupoints (5 mm lateral to ST 36 and 
      LI 11), respectively. The A-NS group was administrated with 0.05 mL of normal 
      saline by acupoint injection into ST 36 and LI 11. Dermatitis severity for dorsal 
      skin of mice was determined using the Severity Scoring of Atopic Dermatitis 
      (SCORAD) every week. The total immunoglobulin E (IgE), interleukin-4 (IL-4), and 
      interferon-gamma (IFN-gamma) cytokine levels in serum were examined by enzyme-linked 
      immunosorbent assay (ELISA). Spleen Th1/Th2 expression were analyzed via flow 
      cytometry and immunohistochemical assay was used to detect T-box expressed in T 
      cell (T-bet) and GATA-binding protein 3 (GATA3) expressions in skin lesions of 
      mice. RESULTS: Compared with the AD group, both A-AHT and sA-AHT reduced the 
      SCORAD index and serum IgE level (P<0.05 or P<0.01); A-AHT, sA-AHT and A-NS 
      down-regulated serum IL-4 level and upregulated IFN-gamma/IL-4 ratio (P<0.05 or 
      P<0.01); A-AHT regulated the Th1/Th2 shift specifically and increased the related 
      transcription factors such as T-bet expression and T-bet/GATA3 ratio (P<0.05). 
      CONCLUSION: A-AHT showed significant effectiveness on the AD model mice, through 
      regulating Th1/Th2 immune responses.
CI  - (c) 2022. The Chinese Journal of Integrated Traditional and Western Medicine Press 
      and Springer-Verlag GmbH Germany, part of Springer Nature.
FAU - Zeng, Zhi-Wen
AU  - Zeng ZW
AD  - School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 
      510515, China.
AD  - Department of Traditional Chinese Medicine, Affiliated Dongguan People's 
      Hospital, Southern Medical University, Dongguan, Guangdong Province, 523058, 
      China.
FAU - Huang, Jin-Quan
AU  - Huang JQ
AD  - School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 
      510515, China.
FAU - Chen, Yong
AU  - Chen Y
AD  - Department of Rheumatology and Immunology, Shenzhen People's Hospital, The Second 
      Clinical Medical College of Jinan University, Shenzhen, Guangdong Province, 
      518020, China.
FAU - Yu, Xiao
AU  - Yu X
AD  - School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, 
      China.
FAU - Zhu, Wei
AU  - Zhu W
AD  - Guangzhou Center for Disease Control and Prevention, Guangzhou, 510515, China.
FAU - Zhang, Dong-Shu
AU  - Zhang DS
AD  - School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 
      510515, China. nyzds@sina.com.
AD  - Department of Traditional Chinese Medicine, Affiliated Dongguan People's 
      Hospital, Southern Medical University, Dongguan, Guangdong Province, 523058, 
      China. nyzds@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20220701
PL  - China
TA  - Chin J Integr Med
JT  - Chinese journal of integrative medicine
JID - 101181180
RN  - 0 (Dinitrobenzenes)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Saline Solution)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - *Acupuncture Points
MH  - Animals
MH  - *Dermatitis, Atopic/chemically induced/therapy
MH  - Dinitrobenzenes
MH  - Dinitrochlorobenzene
MH  - Immunoglobulin E
MH  - Interferon-gamma
MH  - Interleukin-4
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Saline Solution
OTO - NOTNLM
OT  - Th1/Th2
OT  - acupoint autohemotherapy
OT  - atopic dermatitis
OT  - immunoglobulin E
EDAT- 2022/07/02 06:00
MHDA- 2022/07/07 06:00
CRDT- 2022/07/01 11:22
PHST- 2022/03/25 00:00 [accepted]
PHST- 2022/07/01 11:22 [entrez]
PHST- 2022/07/02 06:00 [pubmed]
PHST- 2022/07/07 06:00 [medline]
AID - 10.1007/s11655-022-3579-7 [pii]
AID - 10.1007/s11655-022-3579-7 [doi]
PST - ppublish
SO  - Chin J Integr Med. 2022 Jul;28(7):612-619. doi: 10.1007/s11655-022-3579-7. Epub 
      2022 Jul 1.