PMID- 35777002
OWN - NLM
STAT- MEDLINE
DCOM- 20220706
LR  - 20231213
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Print)
IS  - 0025-7974 (Linking)
VI  - 101
IP  - 26
DP  - 2022 Jul 1
TI  - Association analysis of single nucleotide polymorphisms in autophagy related 7 
      (ATG7) gene in patients with coronary artery disease.
PG  - e29776
LID - 10.1097/MD.0000000000029776 [doi]
LID - e29776
AB  - Recent experimental studies sparked the involvement of autophagy-related 7 (ATG7) 
      in the development of atherosclerosis. However, the genetic variants and their 
      association with coronary artery disease (CAD) are still to be unveiled. 
      Therefore, we aimed to design a retrospective case-control study for the analysis 
      of ATG7 gene polymorphisms and their association with CAD among the subjects 
      originating from Pakistan. The ATG7 noncoding polymorphisms (rs1375206; 
      Chr3:11297643 C/G and rs550744886; Chr3:11272004 C/G) were examined in 600 
      subjects, including 300 individuals diagnosed with CAD. Arginase-1 (ARG1) and 
      nitric oxide metabolites were measured by the colorimetric enzymatic assay. 
      Genotyping of noncoding ATG7 polymorphisms was accomplished by the polymerase 
      chain reaction-restriction fragment length polymorphism method. A significant 
      association of ATG7 (rs1375206 and rs550744886) was observed in individuals 
      exhibiting CAD (P < .0001, for each single-nucleotide polymorphism). Moreover, 
      variant allele G at both loci showed high occurrence and significant association 
      with the disease phenotype as compared to the wild-type allele (odds ratio [OR] = 
      2.03, P < .0001 and OR = 2.08, P < .001, respectively). Variant genotypes at ATG7 
      rs1375206 and rs550744886 showed significant association with high concentrations 
      of ARG1 and low nitric oxide metabolites among the patients (P < .0001 for each). 
      A significant difference was noted in the distribution of the haplotype G-G, 
      mapped at Chr3:11297643-11272004 between cases and controls (P < .0001). The 
      study concludes that ATG7 polymorphisms are among the risk factors for CAD in the 
      subjects from Pakistan. The study thus highlights the novel risk factors for high 
      incidents of the disease and reported for the first time to the best of our 
      knowledge.
CI  - Copyright (c) 2022 the Author(s). Published by Wolters Kluwer Health, Inc.
FAU - Sarosh, Moomal
AU  - Sarosh M
AD  - Department of Biosciences, COMSATS University Islamabad, Islamabad, Pakistan.
FAU - Nurulain, Syed Muhammad
AU  - Nurulain SM
FAU - Shah, Syed Tahir Abbas
AU  - Shah STA
FAU - Jadoon Khan, Muhammad
AU  - Jadoon Khan M
FAU - Muneer, Zahid
AU  - Muneer Z
FAU - Bibi, Nazia
AU  - Bibi N
FAU - Shah, Syed Fawad Ali
AU  - Shah SFA
FAU - Hussain, Sabir
AU  - Hussain S
LA  - eng
PT  - Journal Article
DEP - 20220701
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 6.2.1.45 (ATG7 protein, human)
RN  - EC 6.2.1.45 (Autophagy-Related Protein 7)
SB  - IM
MH  - Autophagy
MH  - *Autophagy-Related Protein 7/genetics
MH  - Case-Control Studies
MH  - *Coronary Artery Disease/genetics
MH  - Humans
MH  - Nitric Oxide
MH  - *Polymorphism, Single Nucleotide
MH  - Retrospective Studies
PMC - PMC9239656
COIS- The authors have no conflicts of interest to disclose.
EDAT- 2022/07/02 06:00
MHDA- 2022/07/07 06:00
PMCR- 2022/06/30
CRDT- 2022/07/01 17:52
PHST- 2022/07/01 17:52 [entrez]
PHST- 2022/07/02 06:00 [pubmed]
PHST- 2022/07/07 06:00 [medline]
PHST- 2022/06/30 00:00 [pmc-release]
AID - 00005792-202207010-00019 [pii]
AID - 10.1097/MD.0000000000029776 [doi]
PST - epublish
SO  - Medicine (Baltimore). 2022 Jul 1;101(26):e29776. doi: 
      10.1097/MD.0000000000029776.