PMID- 35777302
OWN - NLM
STAT- MEDLINE
DCOM- 20220811
LR  - 20220816
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 124
IP  - 6
DP  - 2022 Aug
TI  - microRNA-25-3p suppresses osteogenic differentiation of BMSCs in patients with 
      osteoporosis by targeting ITGB3.
PG  - 151926
LID - S0065-1281(22)00085-X [pii]
LID - 10.1016/j.acthis.2022.151926 [doi]
AB  - This study was conducted to investigate the impact of the microRNA 
      (miR)-25-3p/ITGB3 axis on the osteogenic differentiation of bone marrow 
      mesenchymal stem cells (BMSCs) from patients with osteoporosis (OP). BMSCs 
      isolated from the bone marrow of healthy controls and OP patients were identified 
      by flow cytometry, in which ITGB3 mRNA and miR-25-3p expression was detected by 
      RT-qPCR and ITGB3, Runx2, OPN, ALP, and OSX protein expression by western blot. 
      The binding between ITGB3 and miR-25-3p was assessed by dual-luciferase reporter 
      gene and Ago2-RIP assays. BMSC osteogenic differentiation was observed by 
      alizarin red staining and ALP activity. The differentiation of BMSCs to 
      adipocytes and chondrocytes was measured by oil red O staining and alcian blue 
      staining, respectively. BMSCs were successfully isolated from the bone marrow of 
      healthy controls (normal-BMSCs) and OP patients (OP-BMSCs). ITGB3, Runx2, OPN, 
      ALP, and OSX expression was poorer and miR-25-3p expression was higher in 
      OP-BMSCs than in normal-BMSCs. Mechanistically, ITGB3 was negatively targeted by 
      miR-25-3p. After osteogenic, adipogenic, and chondrogenic differentiation of 
      BMSCs were successfully induced, adipogenic differentiation increased and 
      osteogenic and chondrogenic differentiation decreased in OP-BMSCs compared with 
      normal-BMSCs. Overexpression of ITGB3 facilitated mineralized nodule formation 
      and elevated ALP activity and Runx2, OPN, and ALP expression in OP-BMSCs. 
      miR-25-3p upregulation diminished mineralized nodule formation, ALP activity, and 
      Runx2, OPN, and ALP expression in OP-BMSCs and normal-BMSCs, which was annulled 
      by additional ITGB3 overexpression. miR-25-3p targets ITGB3, thereby suppressing 
      osteogenic differentiation of BMSCs from OP patients.
CI  - Copyright (c) 2022 Elsevier GmbH. All rights reserved.
FAU - Yu, Dongping
AU  - Yu D
AD  - Department of Orthopedics, the First Hospital of Nanchang, Nanchang, Jiangxi 
      330008, PR China.
FAU - Li, Zhen
AU  - Li Z
AD  - Department of Pathology, the First Hospital of Changsha, Changsha, Hunan 410005, 
      PR China. Electronic address: lizhen19861102@163.com.
FAU - Cao, Jie
AU  - Cao J
AD  - Department of Digestive, the First Hospital of Nanchang, Nanchang, Jiangxi 
      330008, PR China.
FAU - Shen, Feng
AU  - Shen F
AD  - Department of Orthopedics, the First Hospital of Nanchang, Nanchang, Jiangxi 
      330008, PR China.
FAU - Wei, Guowen
AU  - Wei G
AD  - Department of Orthopedics, the First Hospital of Nanchang, Nanchang, Jiangxi 
      330008, PR China.
LA  - eng
PT  - Journal Article
DEP - 20220628
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (ITGB3 protein, human)
RN  - 0 (Integrin beta3)
RN  - 0 (MIRN25 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Cell Differentiation/genetics
MH  - Cells, Cultured
MH  - Core Binding Factor Alpha 1 Subunit
MH  - Humans
MH  - Integrin beta3/genetics/metabolism
MH  - *Mesenchymal Stem Cells
MH  - *MicroRNAs/genetics/metabolism
MH  - Osteogenesis/genetics
MH  - *Osteoporosis/genetics/metabolism
OTO - NOTNLM
OT  - Bone marrow mesenchymal stem cells
OT  - ITGB3
OT  - MicroRNA-25-3p
OT  - Osteogenic differentiation
OT  - Osteoporosis
EDAT- 2022/07/02 06:00
MHDA- 2022/08/12 06:00
CRDT- 2022/07/01 18:29
PHST- 2022/03/11 00:00 [received]
PHST- 2022/06/10 00:00 [revised]
PHST- 2022/06/13 00:00 [accepted]
PHST- 2022/07/02 06:00 [pubmed]
PHST- 2022/08/12 06:00 [medline]
PHST- 2022/07/01 18:29 [entrez]
AID - S0065-1281(22)00085-X [pii]
AID - 10.1016/j.acthis.2022.151926 [doi]
PST - ppublish
SO  - Acta Histochem. 2022 Aug;124(6):151926. doi: 10.1016/j.acthis.2022.151926. Epub 
      2022 Jun 28.