PMID- 35777357
OWN - NLM
STAT- MEDLINE
DCOM- 20220715
LR  - 20220812
IS  - 2213-6711 (Electronic)
IS  - 2213-6711 (Linking)
VI  - 17
IP  - 7
DP  - 2022 Jul 12
TI  - Small-molecule inhibitor cocktail promotes the proliferation of pre-existing 
      liver progenitor cells.
PG  - 1589-1603
LID - S2213-6711(22)00276-4 [pii]
LID - 10.1016/j.stemcr.2022.05.023 [doi]
AB  - A recent study showed that a cocktail of three small molecules, Y-27632, A83-01, 
      and CHIR99021 (YAC), converts mature hepatocytes (MHs) into proliferative 
      bipotent cells that can be induced into MHs and cholangiocytes in rats. However, 
      when we reproduced these experiments, it was found that bipotent cells may be 
      derived from resident liver progenitor cells (LPCs), whose proliferative activity 
      was promoted by YAC. A simple and efficient sorting scheme was also developed in 
      this study to harvest high-purity and high-yield LPCs. The inducible bipotency of 
      purified LPCs was verified; in addition, they were found to spontaneously 
      differentiate into hepatocytes and cholangiocytes due to changes in proliferative 
      status even without induction. Moreover, during the differentiation process, some 
      hepatocytes spontaneously reconverted to LPCs under certain conditions, such as 
      the release of contact inhibition. These findings may improve our understanding 
      of LPCs and provide a cell source for regenerative medicine.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Fu, Qingjie
AU  - Fu Q
AD  - Department of Gastroenterology and Hepatology, Hokkaido University Graduate 
      School of Medicine, Sapporo 060-8638, Japan.
FAU - Ohnishi, Shunsuke
AU  - Ohnishi S
AD  - Department of Gastroenterology and Hepatology, Hokkaido University Graduate 
      School of Medicine, Sapporo 060-8638, Japan; Laboratory of Molecular and Cellular 
      Medicine, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 
      060-0812, Japan. Electronic address: sonishi@pop.med.hokudai.ac.jp.
FAU - Suda, Goki
AU  - Suda G
AD  - Department of Gastroenterology and Hepatology, Hokkaido University Graduate 
      School of Medicine, Sapporo 060-8638, Japan.
FAU - Sakamoto, Naoya
AU  - Sakamoto N
AD  - Department of Gastroenterology and Hepatology, Hokkaido University Graduate 
      School of Medicine, Sapporo 060-8638, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220630
PL  - United States
TA  - Stem Cell Reports
JT  - Stem cell reports
JID - 101611300
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Hepatocytes
MH  - *Liver
MH  - Rats
MH  - *Stem Cells
PMC - PMC9287679
OTO - NOTNLM
OT  - cell differentiation
OT  - cell proliferation
OT  - liver development
OT  - liver progenitor cells
OT  - regenerative medicine
OT  - small molecules
EDAT- 2022/07/02 06:00
MHDA- 2022/07/16 06:00
PMCR- 2022/06/30
CRDT- 2022/07/01 18:42
PHST- 2021/03/03 00:00 [received]
PHST- 2022/05/31 00:00 [revised]
PHST- 2022/05/31 00:00 [accepted]
PHST- 2022/07/02 06:00 [pubmed]
PHST- 2022/07/16 06:00 [medline]
PHST- 2022/07/01 18:42 [entrez]
PHST- 2022/06/30 00:00 [pmc-release]
AID - S2213-6711(22)00276-4 [pii]
AID - 10.1016/j.stemcr.2022.05.023 [doi]
PST - ppublish
SO  - Stem Cell Reports. 2022 Jul 12;17(7):1589-1603. doi: 
      10.1016/j.stemcr.2022.05.023. Epub 2022 Jun 30.