PMID- 35777794
OWN - NLM
STAT- MEDLINE
DCOM- 20220706
LR  - 20220720
IS  - 1550-8080 (Electronic)
IS  - 0091-7370 (Linking)
VI  - 52
IP  - 3
DP  - 2022 May
TI  - High Circulating Levels of Neutrophil Extracellular Traps Parameters Predicting 
      Poor Outcome in COVID-19.
PG  - 374-381
AB  - OBJECTIVE: Exploration of biomarkers to predict the severity of COVID-19 is 
      important to reduce mortality. Upon COVID-19 infection, neutrophil extracellular 
      traps (NET) are formed, which leads to a cytokine storm and host damage. Hence, 
      the extent of NET formation may reflect disease progression and predict mortality 
      in COVID-19. METHODS: We measured 4 NET parameters - cell-free double stranded 
      DNA (cell-free dsDNA), neutrophil elastase, citrullinated histone H3 (Cit-H3), 
      and histone - DNA complex - in 188 COVID-19 patients and 20 healthy controls. 
      Survivors (n=166) were hospitalized with or without oxygen supplementation, while 
      non-survivors (n=22) expired during in-hospital treatment. RESULTS: Cell-free 
      dsDNA was significantly elevated in non-survivors in comparison with survivors 
      and controls. The survival rate of patients with high levels of cell-free dsDNA, 
      neutrophil elastase, and Cit-H3 was significantly lower than that of patients 
      with low levels. These three markers significantly correlated with inflammatory 
      markers (absolute neutrophil count and C-reactive protein). CONCLUSION: Since the 
      increase in NET parameters indicates the unfavourable course of COVID-19 
      infection, patients predisposed to poor outcome can be rapidly managed through 
      risk stratification by using these NET parameters.
CI  - (c) 2022 by the Association of Clinical Scientists, Inc.
FAU - Kim, Seunghwan
AU  - Kim S
AD  - Department of Laboratory Medicine, Seoul National University College of Medicine.
FAU - Hong, Ki Ho
AU  - Hong KH
AD  - Department of Laboratory Medicine, Yonsei University College of Medicine.
FAU - Gu, Ja-Yoon
AU  - Gu JY
AD  - Department of Laboratory Medicine, Seoul National University College of Medicine.
AD  - Cancer Research Institute, Seoul National University College of Medicine.
FAU - In, Ji Won
AU  - In JW
AD  - Department of Laboratory Medicine, Seoul Medical Center.
FAU - Ahn, Mi Young
AU  - Ahn MY
AD  - Department of Internal Medicine, Seoul Medical Center, Seoul, Korea.
FAU - Kim, Hyun Kyung
AU  - Kim HK
AD  - Department of Laboratory Medicine, Seoul National University College of Medicine 
      lukekhk@snu.ac.kr.
AD  - Cancer Research Institute, Seoul National University College of Medicine.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
RN  - 0 (Biomarkers)
RN  - 0 (Cell-Free Nucleic Acids)
RN  - 0 (Histones)
RN  - EC 3.4.21.37 (Leukocyte Elastase)
SB  - IM
MH  - Biomarkers/metabolism
MH  - *COVID-19/diagnosis
MH  - Cell-Free Nucleic Acids/blood/metabolism
MH  - *Extracellular Traps/metabolism
MH  - Histones/blood/metabolism
MH  - Humans
MH  - Leukocyte Elastase/blood/metabolism
MH  - Neutrophils/metabolism
MH  - Prognosis
OTO - NOTNLM
OT  - Cell-free DNA
OT  - Citrullinated histone H3
OT  - Histone-DNA complex
OT  - Neutrophil elastase
OT  - Neutrophil extracellular trap
EDAT- 2022/07/02 06:00
MHDA- 2022/07/07 06:00
CRDT- 2022/07/01 20:43
PHST- 2022/07/01 20:43 [entrez]
PHST- 2022/07/02 06:00 [pubmed]
PHST- 2022/07/07 06:00 [medline]
AID - 52/3/374 [pii]
PST - ppublish
SO  - Ann Clin Lab Sci. 2022 May;52(3):374-381.