PMID- 35777905
OWN - NLM
STAT- MEDLINE
DCOM- 20220707
LR  - 20220716
IS  - 2090-1224 (Electronic)
IS  - 2090-1232 (Print)
IS  - 2090-1224 (Linking)
VI  - 39
DP  - 2022 Jul
TI  - Rapid and sensitive UHPLC-MS/MS methods for dietary sample analysis of 43 
      mycotoxins in China total diet study.
PG  - 15-47
LID - S2090-1232(21)00204-6 [pii]
LID - 10.1016/j.jare.2021.10.008 [doi]
AB  - INTRODUCTION: Mycotoxins are toxic metabolites produced by fungi that commonly 
      contaminate foods. As recommended by the World Health Organization, total diet 
      study (TDS) is the most efficient and effective way to estimate the dietary 
      intakes of certain chemical substances for general populations. It requires 
      sensitive and reliable analytical methods applicable to a wide range of complex 
      food matrices and ready-to-eat dishes. OBJECTIVES: A novel strategy with high 
      selectivity and sensitivity, incorporating three methods based on 
      ultra-high-performance liquid chromatography coupled with tandem mass 
      spectrometry (UHPLC-MS/MS), was designed for measuring 43 mycotoxins in dietary 
      samples in a China TDS. METHODS: The 43 mycotoxins were divided into 3 groups for 
      analysis to achieve better performance. For each group, an UHPLC-MS/MS method was 
      developed to determine the target compounds after clean-up by solid phase 
      extraction. A total of 21 isotope internal standards were employed for accurate 
      quantitation. Method validation in terms of linearity, selectivity, sensitivity, 
      accuracy, and precision was performed for all the 43 mycotoxins in 12 complex 
      food matrices. RESULTS: The limits of detection (LODs) and limits of quantitation 
      (LOQs) were 0.002-1 ng mL(-1) and 0.006-3 ng mL(-1), respectively. The method 
      recoveries of the 43 mycotoxins spiked in 12 food categories were in the range of 
      60.3%-175.9% after internal standard correction, with relative standard 
      deviations (RSDs) below 13.9%. For practical application, this method was 
      utilized for 72 dietary samples collected from 6 provinces in the 6th China TDS. 
      More than 80% of the samples were found contaminated by mycotoxins. DON, SMC, 
      FB(1), ZEN, BEA, ENNB(1), and ENNB were most detected. CONCLUSIONS: The proposed 
      methods with high sensitivity, accuracy, and robustness provide powerful tools 
      for multi-mycotoxin monitoring and dietary exposure assessment, allowing 43 
      mycotoxins, including some emerging mycotoxins, to be accurately investigated in 
      a total diet study for the first time.
CI  - Copyright (c) 2022. Production and hosting by Elsevier B.V.
FAU - Qiu, Nannan
AU  - Qiu N
AD  - NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food 
      Safety Risk Assessment, Beijing 100021, PR China.
FAU - Sun, Danlei
AU  - Sun D
AD  - NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food 
      Safety Risk Assessment, Beijing 100021, PR China.
FAU - Zhou, Shuang
AU  - Zhou S
AD  - NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food 
      Safety Risk Assessment, Beijing 100021, PR China. Electronic address: 
      szhoupku@gmail.com.
FAU - Li, Jingguang
AU  - Li J
AD  - NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food 
      Safety Risk Assessment, Beijing 100021, PR China.
FAU - Zhao, Yunfeng
AU  - Zhao Y
AD  - NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food 
      Safety Risk Assessment, Beijing 100021, PR China. Electronic address: 
      zhaoyf@cfsa.net.cn.
FAU - Wu, Yongning
AU  - Wu Y
AD  - NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food 
      Safety Risk Assessment, Beijing 100021, PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211019
PL  - Egypt
TA  - J Adv Res
JT  - Journal of advanced research
JID - 101546952
RN  - 0 (Mycotoxins)
SB  - IM
MH  - Chromatography, High Pressure Liquid/methods
MH  - Diet
MH  - *Mycotoxins/analysis/chemistry
MH  - Solid Phase Extraction
MH  - *Tandem Mass Spectrometry/methods
PMC - PMC9264008
OTO - NOTNLM
OT  - Complex food matrices
OT  - Determination
OT  - Mycotoxins
OT  - Total diet study
OT  - UHPLC-MS/MS
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/07/02 06:00
MHDA- 2022/07/08 06:00
PMCR- 2021/10/19
CRDT- 2022/07/01 21:05
PHST- 2021/04/14 00:00 [received]
PHST- 2021/09/27 00:00 [revised]
PHST- 2021/10/16 00:00 [accepted]
PHST- 2022/07/01 21:05 [entrez]
PHST- 2022/07/02 06:00 [pubmed]
PHST- 2022/07/08 06:00 [medline]
PHST- 2021/10/19 00:00 [pmc-release]
AID - S2090-1232(21)00204-6 [pii]
AID - 10.1016/j.jare.2021.10.008 [doi]
PST - ppublish
SO  - J Adv Res. 2022 Jul;39:15-47. doi: 10.1016/j.jare.2021.10.008. Epub 2021 Oct 19.