PMID- 35781013
OWN - NLM
STAT- MEDLINE
DCOM- 20220816
LR  - 20221006
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 135
DP  - 2022 Oct
TI  - Characterization and functional analysis of a myeloid differentiation factor 88 
      in Ostrinia furnacalis Guenee larvae infected by Bacillus thuringiensis.
PG  - 104489
LID - S0145-305X(22)00151-3 [pii]
LID - 10.1016/j.dci.2022.104489 [doi]
AB  - Myeloid differentiation factor 88 (MyD88) is a pivotal adapter protein involved 
      in activating nuclear factor NF-kappaB of the Toll pathway in insect innate immunity. 
      MyD88 has been extensively studied in vertebrates and Drosophila. However, the 
      information ascribed to MyD88 in Lepidoptera is scarce. In the present study, an 
      Ostrinia furnacalis MyD88 (OfMyD88) cDNA was cloned and functionally 
      characterized (GenBank accession no. MN906311). The complete cDNA sequence of 
      OfMyD88 is 804 bp, and contains a 630 bp open reading frame encoding 209 amino 
      acid residues. OfMyD88 has the death domain (DD), an intermediate domain, and the 
      Toll/interleukin 1 receptor (TIR) domain. OfMyD88 was widely expressed in 
      immune-related tissues such as hemocytes, fat body, midgut, and integument, with 
      the highest expression level in hemocytes, and the lowest expression level in 
      integument. To clarify the immune function of MyD88, O. furnacalis larvae were 
      challenged with Bacillus thuringiensis (Bt) through feeding. Bt oral infection 
      had significantly up-regulated the expression of OfMyD88 and immune genes, 
      including PPO2 (prophenoloxidase 2), Attacin, Gloverin, Cecropin, Moricin, GRP3 
      (beta-1, 3-Glucan recognition protein 3), and Lysozyme, and increased the activities 
      of PO and lysozyme in hemolymph of O. furnacalis larvae. Knockdown of OfMyD88 by 
      RNA interference suppressed the expression levels of immune related genes, but 
      not PPO2 in the larvae orally infected with Bt, suggesting that OfMyD88 is 
      involved in defending against Bt invasion through the Toll signaling pathway, but 
      does not affect the PPO expression in O. furnacalis larvae.
CI  - Copyright (c) 2022. Published by Elsevier Ltd.
FAU - Alradi, Mohamed F
AU  - Alradi MF
AD  - Department of Plant Protection, College of Horticulture and Plant Protection, 
      Yangzhou University, Yangzhou, Jiangsu, 225009, China; Department of Medical 
      Entomology, College of Public and Environmental Health, University of Khartoum, 
      Khartoum, Khartoum State, 13314, Sudan.
FAU - Lu, Shiqi
AU  - Lu S
AD  - Department of Plant Protection, College of Horticulture and Plant Protection, 
      Yangzhou University, Yangzhou, Jiangsu, 225009, China.
FAU - Wang, Libao
AU  - Wang L
AD  - Department of Plant Protection, College of Horticulture and Plant Protection, 
      Yangzhou University, Yangzhou, Jiangsu, 225009, China.
FAU - Han, Zhaoyang
AU  - Han Z
AD  - Department of Plant Protection, College of Horticulture and Plant Protection, 
      Yangzhou University, Yangzhou, Jiangsu, 225009, China.
FAU - Elradi, Sana A
AU  - Elradi SA
AD  - Department of Physiology, College of Medicine, University of Khartoum, Khartoum, 
      Khartoum State, 13314, Sudan.
FAU - Khogali, Mawahib K
AU  - Khogali MK
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 
      225009, China; Department of Poultry Production, Faculty of Animal Production, 
      University of Khartoum, Khartoum, Khartoum State, 13314, Sudan.
FAU - Liu, Xu
AU  - Liu X
AD  - Department of Plant Protection, College of Horticulture and Plant Protection, 
      Yangzhou University, Yangzhou, Jiangsu, 225009, China.
FAU - Wei, Xiangyi
AU  - Wei X
AD  - Department of Plant Protection, College of Horticulture and Plant Protection, 
      Yangzhou University, Yangzhou, Jiangsu, 225009, China.
FAU - Chen, Kangkang
AU  - Chen K
AD  - Department of Plant Protection, College of Horticulture and Plant Protection, 
      Yangzhou University, Yangzhou, Jiangsu, 225009, China.
FAU - Li, Shuzhong
AU  - Li S
AD  - Department of Plant Protection, College of Horticulture and Plant Protection, 
      Yangzhou University, Yangzhou, Jiangsu, 225009, China.
FAU - Feng, Congjing
AU  - Feng C
AD  - Department of Plant Protection, College of Horticulture and Plant Protection, 
      Yangzhou University, Yangzhou, Jiangsu, 225009, China. Electronic address: 
      fengcj@yzu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220701
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (DNA, Complementary)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Bacillus thuringiensis/genetics
MH  - DNA, Complementary/genetics
MH  - Larva
MH  - *Moths
MH  - Muramidase/metabolism
MH  - Myeloid Differentiation Factor 88/genetics/metabolism
OTO - NOTNLM
OT  - Antimicrobial peptides
OT  - Lysozyme
OT  - MyD88
OT  - Ostrinia furnacalis
OT  - Phenoloxidase
OT  - Toll pathway
EDAT- 2022/07/06 06:00
MHDA- 2022/08/17 06:00
CRDT- 2022/07/05 08:46
PHST- 2022/03/10 00:00 [received]
PHST- 2022/06/23 00:00 [revised]
PHST- 2022/06/25 00:00 [accepted]
PHST- 2022/07/06 06:00 [pubmed]
PHST- 2022/08/17 06:00 [medline]
PHST- 2022/07/05 08:46 [entrez]
AID - S0145-305X(22)00151-3 [pii]
AID - 10.1016/j.dci.2022.104489 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2022 Oct;135:104489. doi: 10.1016/j.dci.2022.104489. Epub 2022 
      Jul 1.