PMID- 35781053
OWN - NLM
STAT- MEDLINE
DCOM- 20220817
LR  - 20220817
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 127
DP  - 2022 Aug
TI  - Identification and functional analysis of Mannose receptor in Asian swamp eel 
      (Monopterus albus) in response to bacterial infection.
PG  - 463-473
LID - S1050-4648(22)00375-8 [pii]
LID - 10.1016/j.fsi.2022.06.061 [doi]
AB  - Mannose receptor (MR), as a member of the C-type lectin (CLEC) family, plays an 
      important role in the internalize pathogen-associated ligands and activate immune 
      response. In the present study, MR was identified and characterized from Asian 
      swamp eel (Monopterus albus) (namely MaMR). The open reading frame of MaMR was 
      4311 bp in length encoding 1437 amino acids of a  approximately 162.308 kDa protein, including 
      a cysteine-rich (CR) domain, a fibronectin type II (FNII) domain, eight C-type 
      lectin-like domains (CTLDs), a transmembrane domain and a short cytoplasmic 
      domain. Phylogenetic analysis indicated that MaMR shared the highest similarity 
      with that of Paralichthys olivaceus. The expression of MaMR was found in all the 
      examined tissues, with the highest expression in the spleen and kidney. After 
      injection with Edwardsiella tarda, the transcript level of MaMR was initially 
      reduced and then significantly elevated in the liver, spleen, foregut and 
      hindgut. In the isolated peripheral blood leukocytes, the expression of MaMR was 
      significantly induced post stimulated with LPS and LTA. Then the MaMR-CTLD4-8 
      recombinant protein was purified. Bacterial agglutination and binding assay 
      showed that rMaMR-CTLD4-8 could bind with both Gram-positive and Gram-negative 
      bacteria and agglutinate bacteria in the presence of calcium in vitro. Further 
      analysis revealed that MaMR and TLR2 coordinately induced the expression of TRAF6 
      and promoted the phosphorylation level of p65, leading to the expression of 
      proinflammatory cytokines il-1beta and tnf-alpha in EPC cells. Taken together, these 
      results reveal that MaMR plays an important role in the immune response of fish 
      to pathogen infections.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Liu, Rongrong
AU  - Liu R
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No.88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Qi, Yue
AU  - Qi Y
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No.88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Zhai, Yaqing
AU  - Zhai Y
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No.88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Li, Hua
AU  - Li H
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No.88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - An, Liguo
AU  - An L
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No.88 East Wenhua Road, Jinan, 250014, 
      China.
FAU - Yang, Guiwen
AU  - Yang G
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No.88 East Wenhua Road, Jinan, 250014, 
      China. Electronic address: yanggw@sdnu.edu.cn.
FAU - Shan, Shijuan
AU  - Shan S
AD  - Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life 
      Sciences, Shandong Normal University, No.88 East Wenhua Road, Jinan, 250014, 
      China. Electronic address: shansj@sdnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220630
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fish Proteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Mannose Receptor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anti-Bacterial Agents
MH  - *Bacterial Infections
MH  - *Fish Diseases
MH  - Fish Proteins/chemistry
MH  - Gene Expression Regulation
MH  - Gram-Negative Bacteria
MH  - Gram-Positive Bacteria
MH  - Lectins, C-Type
MH  - Mannose Receptor
MH  - Phylogeny
MH  - *Smegmamorpha
OTO - NOTNLM
OT  - Expression profile
OT  - Inflammatory response
OT  - Mannose receptor
OT  - Microbial-binding
OT  - Monopterus albus
EDAT- 2022/07/06 06:00
MHDA- 2022/08/18 06:00
CRDT- 2022/07/05 08:47
PHST- 2022/04/17 00:00 [received]
PHST- 2022/06/18 00:00 [revised]
PHST- 2022/06/25 00:00 [accepted]
PHST- 2022/07/06 06:00 [pubmed]
PHST- 2022/08/18 06:00 [medline]
PHST- 2022/07/05 08:47 [entrez]
AID - S1050-4648(22)00375-8 [pii]
AID - 10.1016/j.fsi.2022.06.061 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2022 Aug;127:463-473. doi: 10.1016/j.fsi.2022.06.061. 
      Epub 2022 Jun 30.