PMID- 35781650
OWN - NLM
STAT- MEDLINE
DCOM- 20230201
LR  - 20230202
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 478
IP  - 2
DP  - 2023 Feb
TI  - Pluronic F127-liposome-encapsulated curcumin activates Nrf2/Keap1 signaling 
      pathway to promote cell migration of HaCaT cells.
PG  - 241-247
LID - 10.1007/s11010-022-04481-6 [doi]
AB  - Curcumin (CUR) is an extract of Curcuma longa Linn., which has various 
      pharmacological activities. The instability, low water solubility and 
      bioavailability of CUR greatly limit its clinical application. This work prepared 
      Pluronic F127-liposome-encapsulated curcumin (CUR-LIP-F127) and explored its 
      functional role in wound healing. Liposome-encapsulated curcumin (CUR-LIP) and 
      CUR-LIP-F127 were prepared. Human keratinocyte cell line (HaCaT) was treated with 
      CUR, Pluronic F127-liposome (LIP-F127) and CUR-LIP-F127, or combined with ML385 
      (Nrf2 inhibitor). The expression of mRNAs and proteins was detected by 
      quantitative real-time PCR and western blotting. MTT and wound healing assays 
      were performed to detect cell viability and migration. CUR, LIP-F127 and 
      CUR-LIP-F127 all had no influence on cell viability of HaCaT cells. CUR-LIP-F127 
      treatment significantly accelerated cell migration and enhanced the expression of 
      nuclear factor erythroid-related factor 2 (Nrf2) and kelch-like erythroid 
      cell-derived protein 1 (Keap1) in HaCaT cells with respect to CUR or LIP-F127 
      treatment. ML385 treatment impaired CUR-LIP-F127-mediated promotion of migration 
      and up-regulation of Nrf2 and Keap1 in HaCaT cells. This work demonstrated that 
      CUR-LIP-F127 activated Nrf2/Keap1 signaling pathway to promote migration of HaCaT 
      cells, suggesting that CUR-LIP-F127 may contribute to wound healing.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Zhou, Quan
AU  - Zhou Q
AD  - Department of Emergency, The First Hospital of Hunan University of Chinese 
      MedicineYuhua DistrictHunan Province, No. 95, Shaoshan Middle Road, Changsha, 
      410000, People's Republic of China.
FAU - Cai, Xu
AU  - Cai X
AD  - Department of Orthopaedic, The First Hospital of Hunan University of Chinese 
      Medicine, Hunan Province, Changsha, 410000, People's Republic of China.
FAU - Huang, Ying
AU  - Huang Y
AD  - Department of Neurosurgery, Guangdong Province, Shenzhen Sami International 
      Medical Center, Shenzhen, 518038, People's Republic of China.
FAU - Zhou, Youliang
AU  - Zhou Y
AUID- ORCID: 0000-0002-9063-3906
AD  - Department of Emergency, The First Hospital of Hunan University of Chinese 
      MedicineYuhua DistrictHunan Province, No. 95, Shaoshan Middle Road, Changsha, 
      410000, People's Republic of China. zhouyouliang0125@163.com.
LA  - eng
GR  - 202104101005/Hunan Provincial Health Commission/
GR  - 2021227/Hunan Administration of traditional Chinese Medicine/
PT  - Journal Article
DEP - 20220704
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - IT942ZTH98 (Curcumin)
RN  - 0 (Liposomes)
RN  - 9038-95-3 (UCON 50-HB-5100)
RN  - 106392-12-5 (Poloxamer)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Kelch-Like ECH-Associated Protein 1)
RN  - 0 (KEAP1 protein, human)
SB  - IM
MH  - Humans
MH  - *Curcumin/pharmacology
MH  - Liposomes
MH  - Poloxamer
MH  - NF-E2-Related Factor 2
MH  - HaCaT Cells
MH  - Kelch-Like ECH-Associated Protein 1
MH  - Signal Transduction
OTO - NOTNLM
OT  - Curcumin
OT  - Liposome
OT  - Migration
OT  - Nrf2/Keap1 signaling pathway
OT  - Pluronic F127
OT  - Wound healing
EDAT- 2022/07/06 06:00
MHDA- 2023/02/02 06:00
CRDT- 2022/07/05 09:21
PHST- 2022/02/10 00:00 [received]
PHST- 2022/05/13 00:00 [accepted]
PHST- 2022/07/06 06:00 [pubmed]
PHST- 2023/02/02 06:00 [medline]
PHST- 2022/07/05 09:21 [entrez]
AID - 10.1007/s11010-022-04481-6 [pii]
AID - 10.1007/s11010-022-04481-6 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2023 Feb;478(2):241-247. doi: 10.1007/s11010-022-04481-6. Epub 
      2022 Jul 4.