PMID- 35785031
OWN - NLM
STAT- MEDLINE
DCOM- 20220706
LR  - 20220716
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2022
DP  - 2022
TI  - Improvement of Myocardial Cell Injury by miR-199a-3p/mTOR Axis through Regulating 
      Cell Apoptosis and Autophagy.
PG  - 1642301
LID - 10.1155/2022/1642301 [doi]
LID - 1642301
AB  - BACKGROUND: Myocardial ischemia-reperfusion injury (MIRI) is characterized by its 
      high incidence rate and mortality. miR-199a-3p is thought to be strongly linked 
      with the development of some myocardial diseases, but the influence of 
      miR-199a-3p in MIRI remains unclear. METHODS: AC16 cells were used. The 
      concentrations of mammalian target of rapamycin (mTOR), light chain 3 II/light 
      chain 3 I, and Beclin-1 were detected with western blotting and qRT-PCR. The 
      binding site between mTOR and miR-199a-3p was evaluated via luciferase report 
      assay. Cell apoptosis was evaluated through flow cytometry. RESULTS: Knockdown of 
      miR-199a-3p accelerated the myocardial cell injury after L-oxygen treatment. 
      Increased expression of mTOR and suppressed autophagy were observed after 
      knockdown of miR-199a-3p. Knockdown of miR-199a-3p or overexpression of mTOR 
      greatly aggravated cell injury through inhibiting autophagy. Conclusions. This 
      study might be helpful for the therapeutic method of MIRI through by regulating 
      miR-199a-3p/mTOR.
CI  - Copyright (c) 2022 Weixiong Wu et al.
FAU - Wu, Weixiong
AU  - Wu W
AD  - Department of Cardiovascular Surgery, Fujian Medical University Union Hospital, 
      No 29 Xinquan Road, 350001 Fuzhou, China.
FAU - Chen, Xingfeng
AU  - Chen X
AD  - Department of Cardiovascular Surgery, Fujian Medical University Union Hospital, 
      No 29 Xinquan Road, 350001 Fuzhou, China.
FAU - Hu, Qingyang
AU  - Hu Q
AD  - Department of Cardiovascular Surgery, Fujian Medical University Union Hospital, 
      No 29 Xinquan Road, 350001 Fuzhou, China.
FAU - Wang, Xuefei
AU  - Wang X
AD  - Department of Cardiovascular Surgery, Fujian Medical University Union Hospital, 
      No 29 Xinquan Road, 350001 Fuzhou, China.
FAU - Zhu, Jingyu
AU  - Zhu J
AD  - Department of Cardiovascular Surgery, Fujian Medical University Union Hospital, 
      No 29 Xinquan Road, 350001 Fuzhou, China.
FAU - Li, Qianzhen
AU  - Li Q
AUID- ORCID: 0000-0001-7414-6211
AD  - Department of Cardiovascular Surgery, Fujian Medical University Union Hospital, 
      No 29 Xinquan Road, 350001 Fuzhou, China.
AD  - Fujian Key Laboratory of Vascular Aging, Fujian Medical University, No 29 Xinquan 
      Road, 350001 Fuzhou, China.
AD  - Key Laboratory of Cardio-Thoracic Surgery, Fujian Medical University, No 29 
      Xinquan Road, 350001 Fuzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20220622
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (MicroRNAs)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Apoptosis/genetics
MH  - Autophagy/genetics
MH  - *MicroRNAs/genetics/metabolism
MH  - Myocytes, Cardiac/metabolism
MH  - TOR Serine-Threonine Kinases/metabolism
PMC - PMC9242751
COIS- The authors have no conflict of interest.
EDAT- 2022/07/06 06:00
MHDA- 2022/07/07 06:00
PMCR- 2022/06/22
CRDT- 2022/07/05 10:34
PHST- 2022/04/27 00:00 [received]
PHST- 2022/05/17 00:00 [revised]
PHST- 2022/05/23 00:00 [accepted]
PHST- 2022/07/05 10:34 [entrez]
PHST- 2022/07/06 06:00 [pubmed]
PHST- 2022/07/07 06:00 [medline]
PHST- 2022/06/22 00:00 [pmc-release]
AID - 10.1155/2022/1642301 [doi]
PST - epublish
SO  - J Immunol Res. 2022 Jun 22;2022:1642301. doi: 10.1155/2022/1642301. eCollection 
      2022.