PMID- 35786481
OWN - NLM
STAT- MEDLINE
DCOM- 20220706
LR  - 20220706
IS  - 1872-2075 (Electronic)
IS  - 1000-3061 (Linking)
VI  - 38
IP  - 6
DP  - 2022 Jun 25
TI  - [Chondrogenic and ameliorated inflammatory effects of chitosan-based biomimetic 
      scaffold loaded with icariin].
PG  - 2308-2321
LID - 10.13345/j.cjb.210838 [doi]
AB  - Icariin (ICA) is a small molecule drug capable of promoting cartilage repair and 
      ameliorating inflammation. Loading ICA into a biomaterial scaffold for cartilage 
      tissue engineering will thus potentially enhance the biological functionality of 
      the engineered scaffold. In this study, short fibers processed from electrospun 
      poly(l-lactide-co-caprolactone) (PLCL) fibers which were prior coated with 
      polydopamine (PDA), were mixed with citric acid doped chitosan solution (CC) for 
      preparing short fibers reinforced chitosan hydrogel (PDA@PLCL/CC) by a 
      freeze-thawing combined freeze-drying method. Thereafter, ICA was loaded into the 
      PDA@PLCL/CC scaffold through physical adsorption to generate a newly engineered 
      biomimetic cartilage scaffold (ICA-PDA@PLCL/CC). Finally, ICA-mediated 
      chondrogenic and ameliorated inflammatory effects of the ICA-PDA@PLCL/CC scaffold 
      were examined in vitro using rabbit chondrocytes. The results showed that the 
      ICA-free PDA@PLCL/CC scaffold possessed appropriate pore size and porosity (> 
      80%), high water absorbance capacity and improved mechanical performance, and 
      also promoted chondrocyte proliferation and adhesion. The ICA-laden 
      ICA-PDA@PLCL/CC scaffold was evidenced to maintain cytomorphology, upregulate the 
      expression of chondrogenic gene (sox-9), glycosaminoglycan gene (gag), and type Ⅱ 
      collagen gene (col Ⅱ) as well as the synthesis of the cartilage matrix. In the 
      presence of a simulated inflammation, the ICA-PDA@PLCL/CC scaffold was found to 
      reduce chondrocyte fibrosis, effectively downregulate the expression of 
      proinflammatory factors interleukin-6 (il-6), interleukin-1 (il-1), and inducible 
      nitric oxide synthase (inos) in chondrocytes. It can also reduce matrix 
      metalloproteinase-3 (mmp-3) expression and promote the synthesis of the 
      extracellular matrix glycosaminoglycan (GAG) and type II collagen (Col II). The 
      newly developed ICA-PDA@PLCL/CC scaffold may find applications in the 
      regeneration and repair of cartilage defects.
FAU - Li, Huijuan
AU  - Li H
AD  - College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 
      Shanghai 201620, China.
FAU - Wang, Xianliu
AU  - Wang X
AD  - College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 
      Shanghai 201620, China.
FAU - Shen, Yanbing
AU  - Shen Y
AD  - College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 
      Shanghai 201620, China.
FAU - Tang, Han
AU  - Tang H
AD  - College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 
      Shanghai 201620, China.
FAU - Tang, Xiaohan
AU  - Tang X
AD  - College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 
      Shanghai 201620, China.
FAU - Zhang, Yanzhong
AU  - Zhang Y
AD  - College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 
      Shanghai 201620, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (Collagen Type II)
RN  - 0 (Flavonoids)
RN  - 0 (Glycosaminoglycans)
RN  - 0 (Polyesters)
RN  - 9012-76-4 (Chitosan)
RN  - VNM47R2QSQ (icariin)
SB  - IM
MH  - Animals
MH  - Biomimetics
MH  - *Chitosan
MH  - Collagen Type II/genetics
MH  - Flavonoids
MH  - Glycosaminoglycans
MH  - Inflammation/prevention & control
MH  - Polyesters
MH  - Rabbits
OTO - NOTNLM
OT  - amelioration of inflammation
OT  - cartilage regeneration
OT  - chitosan hydrogel
OT  - electrospinning
OT  - icariin
EDAT- 2022/07/06 06:00
MHDA- 2022/07/07 06:00
CRDT- 2022/07/05 13:25
PHST- 2022/07/05 13:25 [entrez]
PHST- 2022/07/06 06:00 [pubmed]
PHST- 2022/07/07 06:00 [medline]
AID - 10.13345/j.cjb.210838 [doi]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2022 Jun 25;38(6):2308-2321. doi: 
      10.13345/j.cjb.210838.