PMID- 35787000
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220816
IS  - 2046-3758 (Print)
IS  - 2046-3758 (Electronic)
IS  - 2046-3758 (Linking)
VI  - 11
IP  - 7
DP  - 2022 Jul
TI  - Osteocyte-specific dentin matrix protein 1 : the role of mineralization 
      regulation in low-magnitude high-frequency vibration enhanced osteoporotic 
      fracture healing.
PG  - 465-476
LID - 10.1302/2046-3758.117.BJR-2021-0476.R2 [doi]
AB  - AIMS: There is an increasing concern of osteoporotic fractures in the ageing 
      population. Low-magnitude high-frequency vibration (LMHFV) was shown to 
      significantly enhance osteoporotic fracture healing through alteration of 
      osteocyte lacuno-canalicular network (LCN). Dentin matrix protein 1 (DMP1) in 
      osteocytes is known to be responsible for maintaining the LCN and mineralization. 
      This study aimed to investigate the role of osteocyte-specific DMP1 during 
      osteoporotic fracture healing augmented by LMHFV. METHODS: A metaphyseal fracture 
      was created in the distal femur of ovariectomy-induced osteoporotic Sprague 
      Dawley rats. Rats were randomized to five different groups: 1) DMP1 knockdown 
      (KD), 2) DMP1 KD + vibration (VT), 3) Scramble + VT, 4) VT, and 5) control (CT), 
      where KD was performed by injection of short hairpin RNA (shRNA) into marrow 
      cavity; vibration treatment was conducted at 35 Hz, 0.3 g; 20 minutes/day, five 
      days/week). Assessments included radiography, micro-CT, dynamic histomorphometry 
      and immunohistochemistry on DMP1, sclerostin, E11, and fibroblast growth factor 
      23 (FGF23). In vitro, murine long bone osteocyte-Y4 (MLO-Y4) osteocyte-like cells 
      were randomized as in vivo groupings. DMP1 KD was performed by transfecting cells 
      with shRNA plasmid. Assessments included immunocytochemistry on 
      osteocyte-specific markers as above, and mineralized nodule staining. RESULTS: 
      Healing capacities in DMP1 KD groups were impaired. Results showed that DMP1 KD 
      significantly abolished vibration-enhanced fracture healing at week 6. DMP1 KD 
      significantly altered the expression of osteocyte-specific markers. The lower 
      mineralization rate in DMP1 KD groups indicated that DMP1 knockdown was 
      associated with poor fracture healing process. CONCLUSION: The blockage of DMP1 
      would impair healing outcomes and negate LMHFV-induced enhancement on fracture 
      healing. These findings reveal the importance of DMP1 in response to the 
      mechanical signal during osteoporotic fracture healing. Cite this article: Bone 
      Joint Res 2022;11(7):465-476.
FAU - Li, Meng C M
AU  - Li MCM
AUID- ORCID: 0000-0001-8720-2008
AD  - Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, 
      The Chinese University of Hong Kong, Hong Kong, China.
FAU - Chow, Simon K-H
AU  - Chow SK
AUID- ORCID: 0000-0003-1742-980X
AD  - Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, 
      The Chinese University of Hong Kong, Hong Kong, China.
FAU - Wong, Ronald M Y
AU  - Wong RMY
AUID- ORCID: 0000-0001-5615-6627
AD  - Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, 
      The Chinese University of Hong Kong, Hong Kong, China.
FAU - Chen, Bailing
AU  - Chen B
AD  - Department of Spine Surgery, The First Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou, China.
FAU - Cheng, Jack C Y
AU  - Cheng JCY
AUID- ORCID: 0000-0001-8153-364X
AD  - Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, 
      The Chinese University of Hong Kong, Hong Kong, China.
FAU - Qin, Ling
AU  - Qin L
AUID- ORCID: 0000-0001-6173-6167
AD  - Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, 
      The Chinese University of Hong Kong, Hong Kong, China.
FAU - Cheung, Wing-Hoi
AU  - Cheung WH
AUID- ORCID: 0000-0003-3247-8255
AD  - Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, 
      The Chinese University of Hong Kong, Hong Kong, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Bone Joint Res
JT  - Bone & joint research
JID - 101586057
PMC - PMC9350691
OTO - NOTNLM
OT  - Dentin matrix protein 1
OT  - Fracture healing
OT  - Osteocyte
OT  - Osteoporotic fracture healing
OT  - Vibration
OT  - distal femur
OT  - long bone
OT  - matrix protein
OT  - metaphyseal fracture
OT  - mice models
OT  - osteoporotic fracture healing
OT  - staining
OT  - vibration treatment
EDAT- 2022/07/06 06:00
MHDA- 2022/07/06 06:01
PMCR- 2022/07/13
CRDT- 2022/07/05 17:07
PHST- 2022/07/05 17:07 [entrez]
PHST- 2022/07/06 06:00 [pubmed]
PHST- 2022/07/06 06:01 [medline]
PHST- 2022/07/13 00:00 [pmc-release]
AID - BJR-11-465 [pii]
AID - 10.1302/2046-3758.117.BJR-2021-0476.R2 [doi]
PST - ppublish
SO  - Bone Joint Res. 2022 Jul;11(7):465-476. doi: 
      10.1302/2046-3758.117.BJR-2021-0476.R2.