PMID- 35788050 OWN - NLM STAT- MEDLINE DCOM- 20220825 LR - 20221015 IS - 1567-7257 (Electronic) IS - 1567-1348 (Linking) VI - 103 DP - 2022 Sep TI - Genome analysis of human respiratory syncytial virus in Fujian Province, Southeast China. PG - 105329 LID - S1567-1348(22)00126-5 [pii] LID - 10.1016/j.meegid.2022.105329 [doi] AB - BACKGROUND: Respiratory syncytial virus (RSV) is one of the main causes of acute respiratory infections (ARI) leading to a heavy disease burden. Reports on RSV in China are limited, especially in Fujian Province, and RSV whole-genome sequences in Fujian Province are not reported. This study aimed to explore the genomic characteristics of RSV to provide evidence for the development of vaccines and medicines. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify and obtain the attachment (G) gene and whole-genome fragments from the positive samples. Genetic evolution as well as nucleotide and amino acid homology analyses of the virus sequences were conducted to identify any amino acid mutations. RESULTS: A total of 72 RSV-positive cases were collected, and 53 G gene sequences were obtained using polymerase chain reaction (PCR) amplification. The ON1 and BA11 genotypes were found to be dominant using the Basic Local Alignment Search Tool (BLAST) on the NCBI website. The 40 genotype ON1 sequences had high nucleotide identity (95.3%-99.8%) and amino acid similarity (92.5%-100%), whereas the 13 BA11 genotype sequenceshad 97.3% - 99.6% nucleotide identity and 94.8% - 99.7% amino acid similarity. Compared to the ON1 prototype (JN257693) and BA11 prototype (AY333364), the obtained sequences had no nucleotide insertions or deletions, indicating high similarity among the samples. A total of 17 RSV whole genome sequences were obtained, 10 of which were genotype ON1 and seven were genotype BA11. Certain amino acid mutations were found in the antigen site and epitope of the fusion (F) protein but not in the G protein. Glycosylation analyses of specific RSV genes revealed high positive selection rates for the gene, and the N- and O-linked glycosylation sequences in the F gene were relatively conserved. CONCLUSIONS: From July 2018 to January 2020, ON1 and BA11 were the most prevalent RSV genotypes in Fujian Province. A high nucleotide identity and amino acid similarity were observed between the reference strain and the obtained strains, as well as among the sequences of the obtained isotypes. Certain amino acid mutations occur at the antigen site and the epitope of the F protein. CI - Copyright (c) 2022 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Chen, Guangmin AU - Chen G AD - The Practice Base on the School of Public Health, Fujian Medical University, Fuzhou 350001, China; Fujian Provincial Center for Disease Control & Prevention, Fuzhou 350001, China; Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou 350001, China. FAU - Lan, Meifang AU - Lan M AD - The Practice Base on the School of Public Health, Fujian Medical University, Fuzhou 350001, China; Fujian Provincial Center for Disease Control & Prevention, Fuzhou 350001, China; Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou 350001, China. FAU - Lin, Sixian AU - Lin S AD - The Practice Base on the School of Public Health, Fujian Medical University, Fuzhou 350001, China; Fujian Provincial Center for Disease Control & Prevention, Fuzhou 350001, China; Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou 350001, China. FAU - Zhang, Yanhua AU - Zhang Y AD - The Practice Base on the School of Public Health, Fujian Medical University, Fuzhou 350001, China; Fujian Provincial Center for Disease Control & Prevention, Fuzhou 350001, China; Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou 350001, China. FAU - Zhang, Dongjuan AU - Zhang D AD - The Practice Base on the School of Public Health, Fujian Medical University, Fuzhou 350001, China; Fujian Provincial Center for Disease Control & Prevention, Fuzhou 350001, China; Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou 350001, China. FAU - Weng, Yuwei AU - Weng Y AD - The Practice Base on the School of Public Health, Fujian Medical University, Fuzhou 350001, China; Fujian Provincial Center for Disease Control & Prevention, Fuzhou 350001, China; Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou 350001, China. FAU - Zheng, Kuicheng AU - Zheng K AD - The Practice Base on the School of Public Health, Fujian Medical University, Fuzhou 350001, China; Fujian Provincial Center for Disease Control & Prevention, Fuzhou 350001, China; Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou 350001, China. Electronic address: kingdadi9909@126.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220703 PL - Netherlands TA - Infect Genet Evol JT - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JID - 101084138 RN - 0 (Amino Acids) RN - 0 (Epitopes) SB - IM MH - Amino Acids/genetics MH - China/epidemiology MH - Epitopes MH - Genotype MH - Humans MH - Infant MH - Phylogeny MH - *Respiratory Syncytial Virus Infections/epidemiology MH - *Respiratory Syncytial Virus, Human/genetics OTO - NOTNLM OT - Acute respiratory infection OT - Respiratory syncytial virus OT - Whole genome EDAT- 2022/07/06 06:00 MHDA- 2022/08/26 06:00 CRDT- 2022/07/05 18:00 PHST- 2022/03/02 00:00 [received] PHST- 2022/06/12 00:00 [revised] PHST- 2022/06/20 00:00 [accepted] PHST- 2022/07/06 06:00 [pubmed] PHST- 2022/08/26 06:00 [medline] PHST- 2022/07/05 18:00 [entrez] AID - S1567-1348(22)00126-5 [pii] AID - 10.1016/j.meegid.2022.105329 [doi] PST - ppublish SO - Infect Genet Evol. 2022 Sep;103:105329. doi: 10.1016/j.meegid.2022.105329. Epub 2022 Jul 3.