PMID- 35789407
OWN - NLM
STAT- MEDLINE
DCOM- 20220706
LR  - 20220706
IS  - 1578-1267 (Electronic)
IS  - 0301-0546 (Linking)
VI  - 50
IP  - 4
DP  - 2022
TI  - Study on the antipruritic mechanism of Zanthoxylum bungeanum and Zanthoxylum 
      schinifolium volatile oil on chronic eczema based on H1R and PAR-2 mediated GRPR 
      pathway.
PG  - 83-96
LID - 10.15586/aei.v50i4.486 [doi]
AB  - OBJECTIVE: To observe the antipruritic effect and mechanism of the volatile oil 
      of Zanthoxylum bungeanum and Zanthoxylum schinifolium on chronic eczema to 
      provide data support for clinical application and new drug development of 
      Zanthoxylum bungeanum and Zanthoxylum schinifolium. METHODS: The model of chronic 
      eczema was established by using 2-dinitrochlorobenzene (DNCB), and the 
      composition and content of volatile oil in Zanthoxylum schinifolium and 
      Zanthoxylum bungeanum was determined by gas chromatography-mass spectrometry 
      (GC-MS). The antipruritic effect by (EASI) score of eczema area and severity 
      index and scratching times was then evaluated. Then, the contents of histamine, 
      gastrin-releasing peptide (GRP), interleukin-4 (IL-4), and immunoglobulin E (IgE) 
      in serum of rats was determined by enzyme-linked immunosorbent assay (ELISA). The 
      tissue morphology was observed by HE staining. The expressions of H1R, PAR-2, 
      TRPV1, TRPA1, and GRPR was then detected by immunohistochemistry, Western blot, 
      and QRT-PCR. RESULTS: The results revealed that there were differences in the 
      composition of volatile oil between Zanthoxylum bungeanum and Zanthoxylum 
      schinifolium. Compared to the model group, the medium-dose group of Zanthoxylum 
      bungeanum and Zanthoxylum schinifolium group significantly increased the 
      difference of EASI score and scratching times, significantly decreased the 
      concentrations of IL-4, IgE, GRP, and histamine, and significantly decreased the 
      expression levels of H1R, PAR-2, TRPV1, and GRPR. The degree of inhibition on the 
      pathological manifestations of chronic eczema was evident. There was no 
      significant difference in antipruritic effect between the two groups. The 
      expression of TRPA1 was inconsistent at the protein and gene level, which needs 
      to be further researched. CONCLUSION: The volatile oil of Zanthoxylum bungeanum 
      and Zanthoxylum schinifolium can reduce the expression of H1R, PAR-2, TRPV1, and 
      GRPR. The mechanism may be through the H1R and PAR-2-mediated GRPR pathway 
      intervention to achieve the effect, both of which have the same antipruritic 
      effect.
FAU - Zhou, Xiang Lu
AU  - Zhou XL
AD  - Institute of traditional Chinese medicine, Shandong Traditional Chinese Medicine 
      University, Jinan, China.
FAU - Chen, Le Le
AU  - Chen LL
AD  - Institute of traditional Chinese medicine, Shandong Traditional Chinese Medicine 
      University, Jinan, China.
FAU - Wang, Jia Feng
AU  - Wang JF
AD  - Institute of traditional Chinese medicine, Shandong Traditional Chinese Medicine 
      University, Jinan, China; wjfeng2000@126.com.
LA  - eng
PT  - Journal Article
DEP - 20220701
PL  - Singapore
TA  - Allergol Immunopathol (Madr)
JT  - Allergologia et immunopathologia
JID - 0370073
RN  - 0 (Antipruritics)
RN  - 0 (Oils, Volatile)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - Antipruritics
MH  - *Eczema
MH  - Histamine
MH  - Immunoglobulin E
MH  - Interleukin-4
MH  - *Oils, Volatile
MH  - Rats
MH  - *Zanthoxylum
OTO - NOTNLM
OT  - Zanthoxylum bungeanum
OT  - Zanthoxylum schinifolium
OT  - chronic eczema
OT  - pruritus
OT  - volatile oil
EDAT- 2022/07/06 06:00
MHDA- 2022/07/07 06:00
CRDT- 2022/07/05 19:35
PHST- 2021/08/02 00:00 [received]
PHST- 2022/11/22 00:00 [accepted]
PHST- 2022/07/05 19:35 [entrez]
PHST- 2022/07/06 06:00 [pubmed]
PHST- 2022/07/07 06:00 [medline]
AID - 10.15586/aei.v50i4.486 [doi]
PST - epublish
SO  - Allergol Immunopathol (Madr). 2022 Jul 1;50(4):83-96. doi: 
      10.15586/aei.v50i4.486. eCollection 2022.