PMID- 35789409
OWN - NLM
STAT- MEDLINE
DCOM- 20220706
LR  - 20220706
IS  - 1578-1267 (Electronic)
IS  - 0301-0546 (Linking)
VI  - 50
IP  - 4
DP  - 2022
TI  - Conditioned medium from the bone marrow mesenchymal stem cells modulates immune 
      response via signal transduction and activator of transcription 6 signaling 
      pathway in an allergic rhinitis mouse model.
PG  - 105-114
LID - 10.15586/aei.v50i4.572 [doi]
AB  - BACKGROUND: Allergic rhinitis (AR) is a common immune disease of the nasal mucosa 
      characterized with immunoglobulin E (IgE)-mediated allergic inflammation after 
      exposure to allergens in susceptible population. Previous reports have 
      demonstrated that the bone marrow mesenchymal stem cells (BMSCs) could reduce 
      allergic inflammation. However, there is little knowledge about whether the 
      culture supernatant of BMSCs (conditioned medium, CM) has similar anti- 
      inflammatory potential in treating AR. OBJECTIVE: The study aimed to evaluate the 
      immunoregulatory effects of conditioned medium derived from BMSCs (BMSC-CM) on 
      allergic inflammation in an AR mouse model. MATERIAL AND METHODS: The AR murine 
      model was induced by repeated sensitization and challenges with ovalbumin (OVA). 
      Subsequently the allergic symptoms of AR mice, cytokine levels, the 
      histopathological features of the nasal mucosa and T helper 1 (Th1) : T helper 2 
      (Th2) cells ratio were evaluated. RESULTS: Treatment with BMSC-CM was found as 
      effective as BMSCs in reducing allergic symptoms and inhibiting eosinophilic 
      infiltration in the nasal mucosa. After BMSC-CM or BMSCs administration, the 
      OVA-specific IgE and interleukin 4 levels in serum decreased and interferon gamma 
      level increased compared with AR mice treated with uncultured fresh medium. Flow 
      cytometry analysis revealed a decrease in Th1:Th2 cells ratio after 
      OVA-sensitization and the ratio was reversed by BMSC-CM and BMSCs treatments. 
      Furthermore, the data revealed that BMSC-CM suppressed the production of signal 
      transduction and activator of transcription 6 (STAT6) at messenger RNA and 
      protein levels in the nasal mucosa. CONCLUSION: BMSC-CM could ameliorate allergic 
      inflammation and regulate the balance of Th cells, and the underlying mechanism 
      was closely related to STAT6 signaling pathway. The immunoregulatory effects of 
      BMSCs could be achieved through paracrine function, and nasal dripping of BMSC-CM 
      might be a novel approach for the treatment of AR.
FAU - Zou, Wentao
AU  - Zou W
AD  - Department of Otolaryngology, Shanghai Tenth People's Hospital, School of 
      Medicine, Tongji University, Shanghai, China.
FAU - Zou, Pei
AU  - Zou P
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Tenth People's 
      Hospital, School of Medicine, Tongji University, Shanghai, China.
FAU - Zhang, Jiaxiong
AU  - Zhang J
AD  - Department of Otolaryngology, Shanghai Tenth People's Hospital, School of 
      Medicine, Tongji University, Shanghai, China.
FAU - Cai, Xiaojing
AU  - Cai X
AD  - Department of Otolaryngology, Shanghai Tenth People's Hospital, School of 
      Medicine, Tongji University, Shanghai, China.
FAU - Mao, Xueying
AU  - Mao X
AD  - Department of Otolaryngology, Shanghai Tenth People's Hospital Chongming Branch, 
      Shanghai, China.
FAU - Liu, Guangpeng
AU  - Liu G
AD  - Department of Plastic and Reconstructive Surgery, Shanghai Tenth People's 
      Hospital, School of Medicine, Tongji University, Shanghai, China; 
      shiyuangpliu@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220701
PL  - Singapore
TA  - Allergol Immunopathol (Madr)
JT  - Allergologia et immunopathologia
JID - 0370073
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Culture Media, Conditioned)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/therapeutic use
MH  - Culture Media, Conditioned/adverse effects
MH  - Disease Models, Animal
MH  - Immunity
MH  - Immunoglobulin E
MH  - Inflammation
MH  - *Mesenchymal Stem Cells
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin
MH  - *Rhinitis, Allergic
MH  - Signal Transduction
OTO - NOTNLM
OT  - allergic rhinitis
OT  - bone marrow mesenchymal stem cells
OT  - conditioned medium
OT  - immune regulation
OT  - signal transduction and activator of transcription 6
COIS- The authors declared no potential conflict of interest.
EDAT- 2022/07/06 06:00
MHDA- 2022/07/07 06:00
CRDT- 2022/07/05 19:35
PHST- 2021/12/26 00:00 [received]
PHST- 2022/05/02 00:00 [accepted]
PHST- 2022/07/05 19:35 [entrez]
PHST- 2022/07/06 06:00 [pubmed]
PHST- 2022/07/07 06:00 [medline]
AID - 10.15586/aei.v50i4.572 [doi]
PST - epublish
SO  - Allergol Immunopathol (Madr). 2022 Jul 1;50(4):105-114. doi: 
      10.15586/aei.v50i4.572. eCollection 2022.