PMID- 35790151 OWN - NLM STAT- MEDLINE DCOM- 20220720 LR - 20220727 IS - 1748-605X (Electronic) IS - 1748-6041 (Linking) VI - 17 IP - 5 DP - 2022 Jul 19 TI - 3D biomaterial P scaffolds carrying umbilical cord mesenchymal stem cells improve biointegration of keratoprosthesis. LID - 10.1088/1748-605X/ac7e98 [doi] AB - Biointegration of a keratoprosthesis (KPro) is critical for the device stability and long-term retention. Biointegration of the KPro device and host tissue takes place between the surrounding corneal graft and the central optic (made by poly (methyl methacrylate)). Our previous clinical results showed that auricular cartilage reinforcement is able to enhance the KPro biointegration. However, the auricular cartilage is non-renewable and difficult to acquire. In this study, we developed a novel type of biomaterial using a three-dimensional porous polyethylene glycol acrylate scaffold (3D biological P-scaffold) carrier with chondrocytes differentiated from induced human umbilical cord mesenchymal stem cells (hUC-MSCs) and tested in rabbit corneas. The results showed hUC-MSCs bear stem cell properties and coule be induced into chondrocytes, P-scaffold is beneficial to the growth and differentiation of hUC-MSCs bothin vivoandin vitro. Besides, after implanting the P-scaffold into the corneal stroma, no serious immune rejection response, such as corneal ulcer or perforation were seen, suggested a good biocompatibility of P-scaffold with the corneal tissue. Moreover, after implanting P-scaffold in together with the differentiated chondrocytes into the rabbit corneal stroma, they significantly increased corneal thickness and strengthened the host cornea, and chondrocytes could stably persist inside the cornea. In summary, the 3D biological P-scaffold carrying differentiated hUC-MSCs could be the preferable material for KPro reinforcement. CI - (c) 2022 IOP Publishing Ltd. FAU - Li, Yueyue AU - Li Y AD - Medical School of Chinese People's Liberation Army, Beijing 100853, People's Republic of China. AD - Department of Ophthalmology, General Hospital of People's Liberation Army, Beijing 100853, People's Republic of China. FAU - Xu, Wenqin AU - Xu W AD - Department of Ophthalmology, General Hospital of People's Liberation Army, Beijing 100853, People's Republic of China. FAU - Li, Qian AU - Li Q AD - Medical School of Chinese People's Liberation Army, Beijing 100853, People's Republic of China. AD - Department of Ophthalmology, General Hospital of People's Liberation Army, Beijing 100853, People's Republic of China. FAU - Li, Xiaoqi AU - Li X AD - Medical School of Chinese People's Liberation Army, Beijing 100853, People's Republic of China. AD - Department of Ophthalmology, General Hospital of People's Liberation Army, Beijing 100853, People's Republic of China. FAU - Li, Junyang AU - Li J AD - Department of Biomedical Engineering, School of Medicine, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, People's Republic of China. FAU - Kang, Li AU - Kang L AD - Department of Ophthalmology, General Hospital of People's Liberation Army, Beijing 100853, People's Republic of China. FAU - Fang, Yifan AU - Fang Y AD - Medical School of Chinese People's Liberation Army, Beijing 100853, People's Republic of China. AD - Department of Ophthalmology, General Hospital of People's Liberation Army, Beijing 100853, People's Republic of China. FAU - Cheng, Shuaishuai AU - Cheng S AD - Medical School of Chinese People's Liberation Army, Beijing 100853, People's Republic of China. FAU - Zhao, Peng AU - Zhao P AD - Department of Biomedical Engineering, School of Medicine, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, People's Republic of China. FAU - Jiang, Shumeng AU - Jiang S AD - Department of Biomedical Engineering, School of Medicine, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, People's Republic of China. FAU - Liu, Wei AU - Liu W AD - Department of Biomedical Engineering, School of Medicine, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, People's Republic of China. FAU - Yan, Xiaojun AU - Yan X AD - Department of Biomedical Engineering, School of Medicine, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, People's Republic of China. FAU - Du, Yanan AU - Du Y AUID- ORCID: 0000-0003-2627-9727 AD - Department of Biomedical Engineering, School of Medicine, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, People's Republic of China. FAU - Wang, Liqiang AU - Wang L AD - Medical School of Chinese People's Liberation Army, Beijing 100853, People's Republic of China. AD - Department of Ophthalmology, General Hospital of People's Liberation Army, Beijing 100853, People's Republic of China. FAU - Huang, Yifei AU - Huang Y AUID- ORCID: 0000-0002-1078-2515 AD - Medical School of Chinese People's Liberation Army, Beijing 100853, People's Republic of China. AD - Department of Ophthalmology, General Hospital of People's Liberation Army, Beijing 100853, People's Republic of China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220719 PL - England TA - Biomed Mater JT - Biomedical materials (Bristol, England) JID - 101285195 RN - 0 (Biocompatible Materials) SB - IM MH - Animals MH - Biocompatible Materials MH - Cell Differentiation MH - Cornea MH - *Corneal Diseases MH - Humans MH - *Mesenchymal Stem Cell Transplantation MH - *Mesenchymal Stem Cells MH - Prostheses and Implants MH - Rabbits MH - Umbilical Cord OTO - NOTNLM OT - 3D biological scaffold OT - histocompatibility OT - keratoprosthesis OT - umbilical cord mesenchymal stem cells EDAT- 2022/07/06 06:00 MHDA- 2022/07/22 06:00 CRDT- 2022/07/05 20:18 PHST- 2022/01/14 00:00 [received] PHST- 2022/07/05 00:00 [accepted] PHST- 2022/07/06 06:00 [pubmed] PHST- 2022/07/22 06:00 [medline] PHST- 2022/07/05 20:18 [entrez] AID - 10.1088/1748-605X/ac7e98 [doi] PST - epublish SO - Biomed Mater. 2022 Jul 19;17(5). doi: 10.1088/1748-605X/ac7e98.