PMID- 35791074
OWN - NLM
STAT- MEDLINE
DCOM- 20220729
LR  - 20221214
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Print)
IS  - 1017-7825 (Linking)
VI  - 32
IP  - 7
DP  - 2022 Jul 28
TI  - CircCOL1A2 Sponges MiR-1286 to Promote Cell Invasion and Migration of Gastric 
      Cancer by Elevating Expression of USP10 to Downregulate RFC2 Ubiquitination 
      Level.
PG  - 938-948
LID - 10.4014/jmb.2112.12044 [doi]
AB  - Gastric cancers (GC) are generally malignant tumors, occurring with high 
      incidence and threatening public health around the world. Circular RNAs 
      (circRNAs) play crucial roles in modulating various cancers, including GC. 
      However, the functions of circRNAs and their regulatory mechanism in colorectal 
      cancer (CRC) remain largely unknown. This study focuses on both the role of 
      circCOL1A2 in CRC progression as well as its downstream molecular mechanism. 
      Quantitative polymerase chain reaction (qPCR) and western blot were adopted for 
      gene expression analysis. Functional experiments were performed to study the 
      biological functions. Fluorescence in situ hybridization (FISH) and subcellular 
      fraction assays were employed to detect the subcellular distribution. Luciferase 
      reporter, RNA-binding protein immunoprecipitation (RIP), co-immunoprecipitation 
      (Co-IP), RNA pull-down, and immunofluorescence (IF) and immunoprecipitation (IP) 
      assays were used to explore the underlying mechanisms. Our results found 
      circCOL1A2 to be not only upregulated in GC cells, but that it also propels the 
      migration and invasion of GC cells. CircCOL1A2 functions as a competing 
      endogenous RNA (ceRNA) by sequestering microRNA-1286 (miR-1286) to modulate 
      ubiquitin-specific peptidase 10 (USP10), which in turn spurs the migration and 
      invasion of GC cells by regulating RFC2. In sum, CircCOL1A2 sponges miR-1286 to 
      promote cell invasion and migration of GC by elevating the expression of USP10 to 
      downregulate the level of RFC2 ubiquitination. Our study offers a potential novel 
      target for the early diagnosis and treatment of GC.
FAU - Li, Hang
AU  - Li H
AD  - Gastroenterology and Hepatobiliary Surgery, The Affiliated Hospital of Hangzhou 
      Normal University, Hangzhou 310015, Zhejiang, P.R.China.
FAU - Chai, Lixin
AU  - Chai L
AD  - Gastroenterology and Hepatobiliary Surgery, The Affiliated Hospital of Hangzhou 
      Normal University, Hangzhou 310015, Zhejiang, P.R.China.
FAU - Ding, Zujun
AU  - Ding Z
AD  - Gastroenterology and Hepatobiliary Surgery, The Affiliated Hospital of Hangzhou 
      Normal University, Hangzhou 310015, Zhejiang, P.R.China.
FAU - He, Huabo
AU  - He H
AD  - Gastroenterology and Hepatobiliary Surgery, The Affiliated Hospital of Hangzhou 
      Normal University, Hangzhou 310015, Zhejiang, P.R.China.
LA  - eng
PT  - Journal Article
DEP - 20220511
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (MicroRNAs)
RN  - 0 (RFC2 protein, human)
RN  - 0 (RNA, Circular)
RN  - 0 (USP10 protein, human)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
RN  - EC 3.6.4.- (Replication Protein C)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs/genetics
MH  - *RNA, Circular/genetics
MH  - *Replication Protein C/genetics/metabolism
MH  - *Stomach Neoplasms/pathology
MH  - *Ubiquitin Thiolesterase/genetics/metabolism
MH  - Ubiquitination
PMC - PMC9628928
OTO - NOTNLM
OT  - Gastric cancer
OT  - RFC2
OT  - USP10
OT  - circCOL1A2
OT  - miR-1286
COIS- Conflict of Interest The authors have no financial conflicts of interest to 
      declare.
EDAT- 2022/07/07 06:00
MHDA- 2022/07/30 06:00
PMCR- 2022/05/11
CRDT- 2022/07/06 00:34
PHST- 2021/12/27 00:00 [received]
PHST- 2022/04/29 00:00 [revised]
PHST- 2022/05/07 00:00 [accepted]
PHST- 2022/07/07 06:00 [pubmed]
PHST- 2022/07/30 06:00 [medline]
PHST- 2022/07/06 00:34 [entrez]
PHST- 2022/05/11 00:00 [pmc-release]
AID - jmb.2112.12044 [pii]
AID - jmb-32-7-938 [pii]
AID - 10.4014/jmb.2112.12044 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2022 Jul 28;32(7):938-948. doi: 10.4014/jmb.2112.12044. 
      Epub 2022 May 11.