PMID- 35792764
OWN - NLM
STAT- MEDLINE
DCOM- 20220726
LR  - 20220727
IS  - 1939-1676 (Electronic)
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 36
IP  - 4
DP  - 2022 Jul
TI  - Effect of leukoreduction on inflammation in critically ill dogs receiving red 
      blood cell transfusions: A randomized blinded controlled clinical trial.
PG  - 1248-1257
LID - 10.1111/jvim.16487 [doi]
AB  - BACKGROUND: Prestorage leukoreduction of red blood cell (RBC) bags prevents 
      accumulation of pro-inflammatory mediators and experimentally attenuates 
      post-transfusion inflammation in healthy dogs. However, the effect of 
      leukoreduction on post-transfusion inflammation in critically ill dogs is 
      unclear. HYPOTHESIS: Dogs transfused with leukoreduced (LR) RBC will have lower 
      concentrations of leukocytes, interleukin (IL)-6, IL-8, monocyte chemoattractant 
      protein-1 (MCP-1), and C-reactive protein (CRP) within 24 hours of 
      post-transfusion compared to dogs transfused with nonleukoreduced (NLR) RBC. 
      ANIMALS: Sixty-one RBC-transfused dogs (LR = 34, NLR = 27). METHODS: Randomized, 
      blinded, controlled preliminary clinical trial. Blood bag processing was 
      randomized to create identically appearing LR and NLR bags. Group allocation 
      occurred with transfusion of the oldest compatible RBC bag. Blood samples were 
      collected pretransfusion and at 8 and 24 hours post-transfusion for leukocyte 
      count, IL-6, IL-8, MCP-1, and CRP. Data were analyzed on an intention-to-treat 
      basis using linear mixed effects models. Significance was set at P < .05. 
      RESULTS: No significant differences were found between groups in concentrations 
      of leukocytes (P = .93), IL-6 (P = .99), IL-8 (P = .75), MCP-1 (P = .69), or CRP 
      (P = .18) over time. Eleven LR dogs (32%) and 4 NLR dogs (15%) were euthanized in 
      the hospital (P = .14). No natural deaths occurred. CONCLUSIONS AND CLINICAL 
      IMPORTANCE: No differences in inflammation biomarker concentrations were detected 
      over time between dogs transfused with LR or NLR RBC, but heterogeneity likely 
      hampered the ability to detect a difference with this sample size. The novel 
      randomization and enrollment protocol was successfully implemented across 2 
      participating institutions and will be easily scaled up for a future multicenter 
      clinical trial.
CI  - (c) 2022 The Authors. Journal of Veterinary Internal Medicine published by Wiley 
      Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
FAU - Claus, Melissa A
AU  - Claus MA
AUID- ORCID: 0000-0003-1529-1480
AD  - Murdoch University, School of Veterinary Medicine, Murdoch, Western Australia, 
      Australia.
FAU - Poh, Denise
AU  - Poh D
AD  - Murdoch University, School of Veterinary Medicine, Murdoch, Western Australia, 
      Australia.
FAU - Smart, Lisa
AU  - Smart L
AD  - Murdoch University, School of Veterinary Medicine, Murdoch, Western Australia, 
      Australia.
FAU - Purcell, Sarah L
AU  - Purcell SL
AD  - University of Queensland, School of Veterinary Science, Gatton, Queensland, 
      Australia.
FAU - Boyd, Corrin J
AU  - Boyd CJ
AD  - Murdoch University, School of Veterinary Medicine, Murdoch, Western Australia, 
      Australia.
FAU - Sharp, Claire R
AU  - Sharp CR
AUID- ORCID: 0000-0002-1797-9783
AD  - Murdoch University, School of Veterinary Medicine, Murdoch, Western Australia, 
      Australia.
LA  - eng
GR  - American College of Veterinary Emergency and Critical Care/
GR  - Animal Hospital at Murdoch University Residency Fund/
GR  - School of Veterinary Life Sciences Research Grant/
PT  - Journal Article
PT  - Multicenter Study
PT  - Randomized Controlled Trial, Veterinary
DEP - 20220706
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Animals
MH  - Blood Preservation/veterinary
MH  - Critical Illness/therapy
MH  - *Dog Diseases/therapy
MH  - Dogs
MH  - *Erythrocyte Transfusion/veterinary
MH  - Inflammation/therapy/veterinary
MH  - Interleukin-6
MH  - Interleukin-8
PMC - PMC9308429
OTO - NOTNLM
OT  - C-reactive protein
OT  - febrile nonhemolytic transfusion reaction
OT  - interleukin-6
OT  - interleukin-8
OT  - leukocytes
OT  - monocyte chemoattractant protein-1
COIS- Authors declare no conflict of interest.
EDAT- 2022/07/07 06:00
MHDA- 2022/07/27 06:00
PMCR- 2022/07/01
CRDT- 2022/07/06 10:27
PHST- 2022/02/24 00:00 [received]
PHST- 2022/06/15 00:00 [accepted]
PHST- 2022/07/07 06:00 [pubmed]
PHST- 2022/07/27 06:00 [medline]
PHST- 2022/07/06 10:27 [entrez]
PHST- 2022/07/01 00:00 [pmc-release]
AID - JVIM16487 [pii]
AID - 10.1111/jvim.16487 [doi]
PST - ppublish
SO  - J Vet Intern Med. 2022 Jul;36(4):1248-1257. doi: 10.1111/jvim.16487. Epub 2022 
      Jul 6.