PMID- 35793888 OWN - NLM STAT- MEDLINE DCOM- 20220708 LR - 20220708 IS - 0255-2930 (Print) IS - 0255-2930 (Linking) VI - 42 IP - 7 DP - 2022 Jul 12 TI - [Effect of acupuncture and moxibustion on skin barrier function in guinea pigs with eczema of skin damp-heat accumulation]. PG - 779-84 LID - 10.13703/j.0255-2930.20210619-k0001 [doi] AB - OBJECTIVE: To observe the effect of acupuncture and moxibustion on the water content of stratum corneum (WCSC), expression of serum inflammatory factors and aquaporin 3 (AQP(3)) in skin, lung and rectum in guinea pigs with eczema of skin damp-heat accumulation, and to explore the possible mechanism of acupuncture and moxibustion for regulating skin barrier function. METHODS: A total of 24 male albino guinea pigs were randomly divided into a blank group (n=6) and a modeling group (n=18). The guinea pigs in the modeling group were induced by 2,4-dinitrochlorobenzene (DNCB) to establish the eczema model of skin damp-heat accumulation. The guinea pigs with successful modeling were further randomly divided into a model group, a medication group and an acupuncture-moxibustion group, 6 guinea pigs in each group. The guinea pigs in the medication group were treated with loratadine tablets (0.8 mg/kg) by gavage, once a day for 7 days; the guinea pigs in the acupuncture-moxibustion group were treated with acupuncture at "Feishu" (BL 13), "Pishu" (BL 20), "Quchi" (LI 11), "Zusanli" (ST 36) and "Xuehai" (SP 10); at the same time, moxibustion was applied at "Feishu" (BL 13) and "Zusanli" (ST 36), moxibustion intervention for 10 min and needle retaining for 15 min at each acupoint, once a day for 7 days. The eczema area and severity index (EASI) score was evaluated before and After intervention, and WCSC and trans-epidermal water loss (TEWL) were measured by skin tester. After intervention, The HE staining was used to observe the changes of skin histomorphology in each group; ELISA was used to measure the contents of serum immunoglobulin E (IgE), interleukin (IL)-4 and IL-17; Western blot was used to measure the protein expression of AQP(3) in skin, lung and rectum. RESULTS: Before the intervention, compared with the blank group, the EASI scores and TEWL were increased in the remaining groups (P<0.01), and the WCSC was decreased (P<0.01). After the intervention, compared with the model group, the EASI scores and TEWL were decreased (P<0.05, P<0.01), and WCSC was increased (P<0.01) in the medication group and the acupuncture-moxibustion group. The epidermal structure in the blank group was complete and the fibers in the dermis were arranged orderly; in the model group, epidermal hyperkeratosis, proliferation of granular layer, spinous cell layer and basal layer, and disordered arrangement of dermal fibers and infiltration of inflammatory cells were observed. The morphological performance in the medication group and the acupuncture-moxibustion group was better than that in the model group. Compared with the blank group, the contents of serum IgE and IL-17 were increased (P<0.01), and the content of serum IL-4 and the protein expression of AQP(3) in skin, lung and rectum were decreased in the model group (P<0.01, P<0.05). Compared with the model group, the contents of serum IgE and IL-17 were decreased and the contents of serum IL-4 were increased in the medication group and the acupuncture-moxibustion group (P<0.01), and the protein expression of AQP(3) in skin, lung and rectum in the acupuncture- moxibustion group were increased (P<0.05). Compared with the medication group, the contents of serum IgE and IL-17 were increased (P<0.01), and the content of serum IL-4 was decreased (P<0.01) in the acupuncture-moxibustion group. CONCLUSION: Acupuncture and moxibustion could improve the epidermal water metabolism and skin tissue morphology in guinea pigs with eczema of skin damp-heat accumulation. Its mechanism may be related to regulating inflammatory factors, up-regulating the expression of AQP(3), and then repairing the skin barrier function. FAU - Wang, Xiu-Mei AU - Wang XM AD - College of TCM, Xinjiang Medical University, Urumqi 830011, China. FAU - Wu, Yi-Ge AU - Wu YG AD - College of TCM, Xinjiang Medical University, Urumqi 830011, China. FAU - He, Yi AU - He Y AD - College of TCM, Xinjiang Medical University, Urumqi 830011, China. FAU - Fan, Li-Na AU - Fan LN AD - College of TCM, Xinjiang Medical University, Urumqi 830011, China. FAU - Zhou, Guang AU - Zhou G AD - College of TCM, Xinjiang Medical University, Urumqi 830011, China. LA - chi PT - Journal Article PL - China TA - Zhongguo Zhen Jiu JT - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JID - 8600658 RN - 0 (Interleukin-17) RN - 059QF0KO0R (Water) RN - 207137-56-2 (Interleukin-4) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - *Acupuncture Therapy MH - *Eczema/therapy MH - Hot Temperature MH - Humans MH - Immunoglobulin E MH - Interleukin-17 MH - Interleukin-4 MH - Male MH - *Moxibustion MH - Water OTO - NOTNLM OT - acupuncture and moxibustion OT - aquaporin 3 (AQP3) OT - eczema, skin damp-heat accumulation OT - epidermal water metabolism EDAT- 2022/07/07 06:00 MHDA- 2022/07/09 06:00 CRDT- 2022/07/06 21:03 PHST- 2022/07/06 21:03 [entrez] PHST- 2022/07/07 06:00 [pubmed] PHST- 2022/07/09 06:00 [medline] AID - 10.13703/j.0255-2930.20210619-k0001 [doi] PST - ppublish SO - Zhongguo Zhen Jiu. 2022 Jul 12;42(7):779-84. doi: 10.13703/j.0255-2930.20210619-k0001.