PMID- 35795773
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2022
DP  - 2022
TI  - Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhance 
      Alveolar Type II Cell Proliferation and Attenuate Lung Inflammation in a Rat 
      Model of Bronchopulmonary Dysplasia.
PG  - 8465294
LID - 10.1155/2022/8465294 [doi]
LID - 8465294
AB  - Although it is known that exosomes derived from human umbilical cord mesenchymal 
      stem cells (hUCMSCs) alleviate hyperoxic lung injury of bronchopulmonary 
      dysplasia (BPD) in animal models, the role of microvesicles (MVs) derived from 
      hUCMSCs in BPD is poorly defined. Furthermore, antenatal inflammation has been 
      linked to high risk of BPD in preterm infants. The purpose of this study was to 
      explore whether MVs derived from hUCMSCs can preserve lung structure and function 
      in an antenatal lipopolysaccharide- (LPS-) induced BPD rat model and to clarify 
      the underlying mechanism. We demonstrate that antenatal LPS induced alveolar 
      simplification, altered lung function, and dysregulated pulmonary vasculature, 
      which restored by hUCMSCs and MVs treatment. Furthermore, MVs were large vesicles 
      with a diameter of 100-900 nanometers and mostly uptaken by alveolar epithelial 
      type II cells (AT2) and macrophages. Compared with the LPS-exposed group, MVs 
      restored the AT2 cell number and SP-C expression in vivo and promoted the 
      proliferation of AT2 cells in vitro. MVs also restored the level of IL-6 and 
      IL-10 in lung homogenate. Additionally, PTEN/AKT and MAPK pathways were 
      associated with the protection of MVs. Taken together, this study suggests MVs 
      derived from hUCMSCs improve lung architecture and function in an antenatal 
      LPS-induced BPD rat model by promoting AT2 cell proliferation and attenuating 
      lung inflammation; thus, MVs provide a promising therapeutic vehicle for BPD 
      treatment.
CI  - Copyright (c) 2022 Ou Zhou et al.
FAU - Zhou, Ou
AU  - Zhou O
AUID- ORCID: 0000-0001-8868-4651
AD  - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of 
      Stem Cell Therapy, Chongqing 400014, China.
FAU - You, Jingyi
AU  - You J
AUID- ORCID: 0000-0003-1694-4017
AD  - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of 
      Stem Cell Therapy, Chongqing 400014, China.
FAU - Xu, Xiaochuan
AU  - Xu X
AUID- ORCID: 0000-0003-1140-7001
AD  - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of 
      Stem Cell Therapy, Chongqing 400014, China.
FAU - Liu, Jiang
AU  - Liu J
AUID- ORCID: 0000-0002-4418-0416
AD  - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of 
      Stem Cell Therapy, Chongqing 400014, China.
FAU - Qiu, Huijun
AU  - Qiu H
AUID- ORCID: 0000-0002-6974-648X
AD  - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of 
      Stem Cell Therapy, Chongqing 400014, China.
FAU - Hao, Chang
AU  - Hao C
AUID- ORCID: 0000-0001-6243-2050
AD  - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of 
      Stem Cell Therapy, Chongqing 400014, China.
FAU - Zou, Wenjing
AU  - Zou W
AUID- ORCID: 0000-0002-0678-3443
AD  - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of 
      Stem Cell Therapy, Chongqing 400014, China.
FAU - Wu, Wenjie
AU  - Wu W
AUID- ORCID: 0000-0001-7408-4401
AD  - Department of Pediatrics, Chongqing Youyoubaobei Women and Children's Hospital, 
      Chongqing 401122, China.
FAU - Fu, Zhou
AU  - Fu Z
AUID- ORCID: 0000-0001-9024-3222
AD  - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of 
      Stem Cell Therapy, Chongqing 400014, China.
FAU - Tian, Daiyin
AU  - Tian D
AUID- ORCID: 0000-0002-6505-6027
AD  - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of 
      Stem Cell Therapy, Chongqing 400014, China.
FAU - Zou, Lin
AU  - Zou L
AUID- ORCID: 0000-0003-1084-3151
AD  - Department of Respiratory Medicine, Children's Hospital of Chongqing Medical 
      University, National Clinical Research Center for Child Health and Disorders, 
      Ministry of Education Key Laboratory of Child Development and Disorders, 
      Chongqing Key Laboratory of Pediatrics, Chongqing Engineering Research Center of 
      Stem Cell Therapy, Chongqing 400014, China.
AD  - Center of Clinical Molecular Medicine, Children's Hospital of Chongqing Medical 
      University, Chongqing 400014, China.
AD  - Clinical Research Unit, Children's Hospital of Shanghai Jiaotong University, 
      Shanghai 200062, China.
LA  - eng
PT  - Journal Article
DEP - 20220627
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC9252687
COIS- The authors declare that they have no competing interests.
EDAT- 2022/07/08 06:00
MHDA- 2022/07/08 06:01
PMCR- 2022/06/27
CRDT- 2022/07/07 02:36
PHST- 2022/01/12 00:00 [received]
PHST- 2022/06/06 00:00 [revised]
PHST- 2022/06/09 00:00 [accepted]
PHST- 2022/07/07 02:36 [entrez]
PHST- 2022/07/08 06:00 [pubmed]
PHST- 2022/07/08 06:01 [medline]
PHST- 2022/06/27 00:00 [pmc-release]
AID - 10.1155/2022/8465294 [doi]
PST - epublish
SO  - Stem Cells Int. 2022 Jun 27;2022:8465294. doi: 10.1155/2022/8465294. eCollection 
      2022.