PMID- 35796761
OWN - NLM
STAT- MEDLINE
DCOM- 20220728
LR  - 20220728
IS  - 1432-2242 (Electronic)
IS  - 0040-5752 (Linking)
VI  - 135
IP  - 8
DP  - 2022 Aug
TI  - Production and cytology of Brassica autoallohexaploids with two and four copies 
      of two subgenomes.
PG  - 2641-2653
LID - 10.1007/s00122-022-04136-6 [doi]
AB  - Different digenomic Brassica autoallohexaploids were produced from the crosses of 
      three allotetraploids and ancestral diploids and characterized for the 
      cytological behavior of two subgenomes with two and four copies. Interspecific 
      hybridization and allopolyploidization present an important pathway for plant 
      evolution and breeding. In this study, different types of digenomic 
      autoallohexaploids with two or four copies of two subgenomes (AAAACC, AACCCC, 
      AAAABB, BBBBCC, BBCCCC) were synthesized by the crosses between three Brassica 
      allotetraploids and their diploid progenitors and the chromosome doubling, and 
      their meiotic behaviors were analyzed by fluorescence in situ hybridization 
      (FISH). These autoallohexaploids showed some variations in pollen fertility and 
      seed-sets and produced both euploid and aneuploid progenies with some chromosomes 
      lost. Two subgenomes in these autoallohexaploids showed some aberrant pairings 
      and segregations, and the degrees of meiotic regularity were negatively 
      associated with the genome affinities. The chromosomes of the subgenome with four 
      copies formed few quadrivalents with the average number < 2, and mainly paired as 
      bivalents, and majority of the chromosomes from the subgenome with two copies 
      gave the expected bivalents. The different extents of the equal and unequal 
      segregations corresponded to the chromosome pairings. The development and 
      cytological investigation of these autoallohexaploids provide not only the new 
      germplasm for genetic research and breeding but also the new clues for the genome 
      behavior and interplay between these subgenomes with different copies.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Cai, Bowei
AU  - Cai B
AD  - National Key Laboratory of Crop Genetic Improvement, National Center of Oil Crop 
      Improvement (Wuhan), College of Plant Science and Technology, Huazhong 
      Agricultural University, Wuhan, 430070, People's Republic of China.
FAU - Wang, Tai
AU  - Wang T
AD  - National Key Laboratory of Crop Genetic Improvement, National Center of Oil Crop 
      Improvement (Wuhan), College of Plant Science and Technology, Huazhong 
      Agricultural University, Wuhan, 430070, People's Republic of China.
FAU - Yue, Fang
AU  - Yue F
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, 
      People's Republic of China.
FAU - Harun, Arrashid
AU  - Harun A
AD  - National Key Laboratory of Crop Genetic Improvement, National Center of Oil Crop 
      Improvement (Wuhan), College of Plant Science and Technology, Huazhong 
      Agricultural University, Wuhan, 430070, People's Republic of China.
FAU - Zhu, Bin
AU  - Zhu B
AD  - School of Life Sciences, Guizhou Normal University, Guiyang, 550025, People's 
      Republic of China.
FAU - Qian, Wei
AU  - Qian W
AD  - College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, 
      People's Republic of China.
FAU - Ge, Xianhong
AU  - Ge X
AUID- ORCID: 0000-0002-3950-210X
AD  - National Key Laboratory of Crop Genetic Improvement, National Center of Oil Crop 
      Improvement (Wuhan), College of Plant Science and Technology, Huazhong 
      Agricultural University, Wuhan, 430070, People's Republic of China. 
      gexianhong@mail.hzau.edu.cn.
FAU - Li, Zaiyun
AU  - Li Z
AD  - National Key Laboratory of Crop Genetic Improvement, National Center of Oil Crop 
      Improvement (Wuhan), College of Plant Science and Technology, Huazhong 
      Agricultural University, Wuhan, 430070, People's Republic of China.
LA  - eng
GR  - 31771827/Natural Science Foundation of China/
PT  - Journal Article
DEP - 20220707
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
SB  - IM
MH  - *Brassica/genetics
MH  - Chromosomes, Plant/genetics
MH  - Genome, Plant
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Breeding
MH  - Polyploidy
EDAT- 2022/07/08 06:00
MHDA- 2022/07/29 06:00
CRDT- 2022/07/07 11:13
PHST- 2021/11/18 00:00 [received]
PHST- 2022/05/22 00:00 [accepted]
PHST- 2022/07/08 06:00 [pubmed]
PHST- 2022/07/29 06:00 [medline]
PHST- 2022/07/07 11:13 [entrez]
AID - 10.1007/s00122-022-04136-6 [pii]
AID - 10.1007/s00122-022-04136-6 [doi]
PST - ppublish
SO  - Theor Appl Genet. 2022 Aug;135(8):2641-2653. doi: 10.1007/s00122-022-04136-6. 
      Epub 2022 Jul 7.