PMID- 35796963
OWN - NLM
STAT- MEDLINE
DCOM- 20220711
LR  - 20220816
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2509
DP  - 2022
TI  - Whole-Mount RNA FISH Combined with Immunofluorescence for the Analysis of the 
      Telomeric Ribonucleoproteins in the Drosophila Germline.
PG  - 157-169
LID - 10.1007/978-1-0716-2380-0_10 [doi]
AB  - The RNA fluorescence in situ hybridization (FISH) technique combined with 
      immunostaining is a powerful method to visualize a specific transcript and a 
      protein of interest simultaneously. Although whole-mount RNA FISH is routinely 
      used to determine RNA intracellular localization, a detailed picture of RNA 
      distribution in complex tissues remains a challenge. The main problem is the 
      various permeability of morphologically different cells within a tissue. We 
      overcome this challenge by developing an approach based on differential 
      permeabilization treatment of tissue specimens. We have tested and optimized 
      conditions for RNA FISH combined with immunofluorescent staining (RNA FISH/IF) to 
      detect the maternal telomeric retrotransposon HeT-A RNPs in the Drosophila 
      ovaries and syncytial embryos. Methods described here are applicable to a broad 
      variety of biological tissue specimens.
CI  - (c) 2022. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Morgunova, Valeriya
AU  - Morgunova V
AD  - Institute of Molecular Genetics of National Research Centre, "Kurchatov 
      Institute", Moscow, Russia.
FAU - Sukhova, Maria M
AU  - Sukhova MM
AD  - Institute of Molecular Genetics of National Research Centre, "Kurchatov 
      Institute", Moscow, Russia.
AD  - Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
FAU - Kalmykova, Alla
AU  - Kalmykova A
AD  - Institute of Molecular Genetics of National Research Centre, "Kurchatov 
      Institute", Moscow, Russia. allakalm@img.ras.ru.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Ribonucleoproteins)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - *Drosophila/genetics/metabolism
MH  - Fluorescent Antibody Technique
MH  - Germ Cells/metabolism
MH  - In Situ Hybridization, Fluorescence/methods
MH  - *RNA/genetics
MH  - Ribonucleoproteins/genetics
OTO - NOTNLM
OT  - Combined RNA FISH and immunofluorescence
OT  - Drosophila
OT  - Embryogenesis
OT  - Germline
OT  - Oogenesis
OT  - Permeabilization
OT  - RNP
OT  - Retrotransposon HeT-A
OT  - Telomere
OT  - piRNA pathway
EDAT- 2022/07/08 06:00
MHDA- 2022/07/12 06:00
CRDT- 2022/07/07 11:21
PHST- 2022/07/07 11:21 [entrez]
PHST- 2022/07/08 06:00 [pubmed]
PHST- 2022/07/12 06:00 [medline]
AID - 10.1007/978-1-0716-2380-0_10 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2509:157-169. doi: 10.1007/978-1-0716-2380-0_10.