PMID- 35797798
OWN - NLM
STAT- MEDLINE
DCOM- 20221018
LR  - 20221018
IS  - 1532-8198 (Electronic)
IS  - 1092-9134 (Linking)
VI  - 60
DP  - 2022 Oct
TI  - Challenges and limitation of MTAP immunohistochemistry in diagnosing desmoplastic 
      mesothelioma/sarcomatoid pleural mesothelioma with desmoplastic features.
PG  - 152004
LID - S1092-9134(22)00106-X [pii]
LID - 10.1016/j.anndiagpath.2022.152004 [doi]
AB  - AIM: Genomic-based ancillary assays including immunohistochemistry (IHC) for 
      BRCA-1 associated protein-1 (BAP1) and methylthioadenosine phosphorylase (MTAP), 
      and fluorescence in situ hybridization (FISH) for CDKN2A are effective for 
      differentiating pleural mesothelioma (PM) from reactive mesothelial 
      proliferations. We previously reported a combination of MTAP and BAP1 IHC 
      effectively distinguishes sarcomatoid PM from fibrous pleuritis (FP). 
      Nevertheless, cases of sarcomatoid PM with desmoplastic features (desmoPM) are 
      encountered where the IHC assessment is unclear. METHODS AND RESULTS: We 
      evaluated assessment of MTAP IHC, BAP1 IHC, and CDKN2A FISH in 20 desmoPM 
      compared to 24 FP. MTAP and BAP1 IHC could not be assessed in 11 (55 %) and 10 
      (50 %) cases, respectively, due to loss or faint immunoreactivity of internal 
      positive control cells, while CDKN2A FISH could be evaluated in all cases. The 
      sensitivities for MTAP loss, BAP1 loss, and CDKN2A homozygous deletion in desmoPM 
      were 40 %, 10 %, and 100 %. A combination of MTAP loss and BAP1 loss yielded 45 % 
      of sensitivity. CONCLUSIONS: MTAP IHC is a useful surrogate diagnostic marker in 
      differentiating ordinary sarcomatoid PM from FP, but its effectiveness is limited 
      in desmoPM. CDKN2A FISH is the most effective diagnostic assays with 100 % 
      sensitivity and specificity in discriminating desmoPM from FP in the facilities 
      where the FISH assay is available.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Sa-Ngiamwibool, Prakasit
AU  - Sa-Ngiamwibool P
AD  - Department of Pathology, Fukuoka University School of Medicine and Hospital, 
      Fukuoka, Japan; Department of Pathology, Faculty of Medicine, Khon Kaen 
      University, Khon Kean, Thailand.
FAU - Hamasaki, Makoto
AU  - Hamasaki M
AD  - Department of Pathology, Fukuoka University School of Medicine and Hospital, 
      Fukuoka, Japan.
FAU - Kinoshita, Yoshiaki
AU  - Kinoshita Y
AD  - Department of Respiratory Medicine, Fukuoka University Chikushi Hospital, 
      Chikushino, Japan.
FAU - Matsumoto, Shinji
AU  - Matsumoto S
AD  - Department of Pathology, Fukuoka University School of Medicine and Hospital, 
      Fukuoka, Japan.
FAU - Sato, Ayuko
AU  - Sato A
AD  - Department of Pathology, Hyogo College of Medicine, Nishinomiya, Japan.
FAU - Tsujimura, Tohru
AU  - Tsujimura T
AD  - Department of Pathology, Hyogo College of Medicine, Nishinomiya, Japan.
FAU - Kasai, Takahiko
AU  - Kasai T
AD  - Department of Pathology, National Hospital Organization Kinki-chuo Chest Medical 
      Center, Osaka, Japan.
FAU - Hiroshima, Kenzo
AU  - Hiroshima K
AD  - Department of Pathology, Tokyo Women's Medical University Yachiyo Medical Center, 
      Yachiyo, Japan.
FAU - Kushitani, Kei
AU  - Kushitani K
AD  - Department of Pathology, Hiroshima University Graduate School of Biomedical and 
      Health Sciences, Hiroshima, Japan.
FAU - Takeshima, Yukio
AU  - Takeshima Y
AD  - Department of Pathology, Hiroshima University Graduate School of Biomedical and 
      Health Sciences, Hiroshima, Japan.
FAU - Kawahara, Kunimitsu
AU  - Kawahara K
AD  - Department of Pathology, Osaka Habikino Medical Center, Osaka, Japan.
FAU - Iwasaki, Akinori
AU  - Iwasaki A
AD  - Department of Thoracic Surgery, Fukuoka University School of Medicine and 
      Hospital, Fukuoka, Japan.
FAU - Nabeshima, Kazuki
AU  - Nabeshima K
AD  - Department of Pathology, Fukuoka University School of Medicine and Hospital, 
      Fukuoka, Japan. Electronic address: kaznabes@fukuoka-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20220630
PL  - United States
TA  - Ann Diagn Pathol
JT  - Annals of diagnostic pathology
JID - 9800503
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - EC 2.4.2.1 (Purine-Nucleoside Phosphorylase)
RN  - EC 2.4.2.28 (5'-methylthioadenosine phosphorylase)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - Biomarkers, Tumor
MH  - Cyclin-Dependent Kinase Inhibitor p16/genetics
MH  - Homozygote
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - *Lung Neoplasms/diagnosis/genetics
MH  - *Mesothelioma/diagnosis/genetics
MH  - *Mesothelioma, Malignant/diagnosis
MH  - *Pleural Neoplasms/diagnosis
MH  - Purine-Nucleoside Phosphorylase
MH  - *Sarcoma
MH  - Sequence Deletion
MH  - *Soft Tissue Neoplasms
MH  - Ubiquitin Thiolesterase/genetics
OTO - NOTNLM
OT  - BAP1
OT  - CDKN2A FISH
OT  - Desmoplastic mesothelioma
OT  - MTAP
OT  - Pleural mesothelioma
OT  - Sarcomatoid mesothelioma
COIS- Declaration of competing interest All authors have no conflicts of interest.
EDAT- 2022/07/08 06:00
MHDA- 2022/10/19 06:00
CRDT- 2022/07/07 18:19
PHST- 2022/05/12 00:00 [received]
PHST- 2022/06/12 00:00 [revised]
PHST- 2022/06/27 00:00 [accepted]
PHST- 2022/07/08 06:00 [pubmed]
PHST- 2022/10/19 06:00 [medline]
PHST- 2022/07/07 18:19 [entrez]
AID - S1092-9134(22)00106-X [pii]
AID - 10.1016/j.anndiagpath.2022.152004 [doi]
PST - ppublish
SO  - Ann Diagn Pathol. 2022 Oct;60:152004. doi: 10.1016/j.anndiagpath.2022.152004. 
      Epub 2022 Jun 30.