PMID- 3579782 OWN - NLM STAT- MEDLINE DCOM- 19870612 LR - 20151119 IS - 0005-2086 (Print) IS - 0005-2086 (Linking) VI - 31 IP - 1 DP - 1987 Jan-Mar TI - Effects of Bordetella avium toxin on turkey tracheal organ cultures as measured with a tetrazolium-reduction assay. PG - 136-9 AB - Turkey tracheal organ cultures (TOCs) were exposed to one of the following Bordetella avium fractions or controls: live B. avium, formalin-killed B. avium, B. avium sonicate, heat-inactivated sonicate, culture supernatant, heat-inactivated culture supernatant, phosphate-buffered saline, or brain-heart infusion broth. After the TOCs were incubated for 2 hours with the bacterial fractions, the cellular metabolism of each TOC was evaluated using a tetrazolium chloride reduction assay, and cellular morphology was determined by light microscopy. Additionally, bacterial fractions and controls were injected into turkeys to test lethality. Although the bacterial sonicate was lethal for turkeys, neither the sonicate nor any other B. avium fraction significantly affected the metabolism or morphology of turkey TOCs. FAU - Van Alstine, W G AU - Van Alstine WG FAU - Arp, L H AU - Arp LH LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PL - United States TA - Avian Dis JT - Avian diseases JID - 0370617 RN - 0 (Bacterial Toxins) RN - 0 (Tetrazolium Salts) SB - IM MH - Animals MH - Bacterial Toxins/*pharmacology MH - *Bordetella MH - Organ Culture Techniques MH - *Tetrazolium Salts MH - Trachea/*drug effects/metabolism/pathology MH - Turkeys/*microbiology EDAT- 1987/01/01 00:00 MHDA- 1987/01/01 00:01 CRDT- 1987/01/01 00:00 PHST- 1987/01/01 00:00 [pubmed] PHST- 1987/01/01 00:01 [medline] PHST- 1987/01/01 00:00 [entrez] PST - ppublish SO - Avian Dis. 1987 Jan-Mar;31(1):136-9.