PMID- 35798039
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230207
IS  - 2765-0189 (Print)
IS  - 2765-0235 (Electronic)
IS  - 2765-0189 (Linking)
VI  - 36
IP  - 2
DP  - 2023 Feb
TI  - Effect of in ovo feeding of gamma-aminobutyric acid combined with embryonic thermal 
      manipulation on hatchability, growth, and hepatic gene expression in broilers.
PG  - 284-294
LID - 10.5713/ab.22.0099 [doi]
AB  - OBJECTIVE: This study investigated the effects of in ovo feeding of 
      gamma-aminobutyric acid (GABA) and embryonic thermal manipulation (ETM) on growth 
      performance, organ indices, plasma biochemical parameters, hepatic antioxidant 
      levels, and expression of lipid metabolism-related genes in broilers. METHODS: 
      Two hundred and fifty eggs were assigned to one of four treatments: control eggs 
      incubated under standard conditions (CON); eggs that received an in ovo injection 
      of 10% GABA on day 17.5 of incubation (G10); thermally manipulated eggs between 
      days 10 and 18 of incubation at 39.6 degrees C for 6 h daily (TM); and eggs that received 
      both treatments during incubation (G10+TM). After 28 days of rearing, five birds 
      per treatment were selected for blood and organ sampling. RESULTS: No differences 
      were found in hatchability or growth parameters among different treatment groups. 
      Hepatic gene expression of catalase (CAT) and glutathione peroxidase 1 (GPx1) was 
      upregulated (p = 0.046 and p = 0.006, respectively) in the G10+TM group, while 
      that of nuclear factor erythroid 2-related factor 2 (NRF2) was upregulated (p = 
      0.039) in the G10 group. In addition, the relative gene expression of NADPH 
      oxidase 1 (NOX1) was significantly lower (p = 0.007) in all treatment groups than 
      that in the CON group. Hepatic fatty acid synthase (FAS) levels and average daily 
      feed intake (ADFI) of last week showed a positive correlation (r = 0.50, p = 
      0.038). In contrast, the relative gene expression of the extracellular fatty 
      acid-binding protein (EXFAB) and peroxisome proliferator-activated receptor-gamma 
      (PPAR-gamma) were positively correlated (r = 0.48, p = 0.042 and r = 0.50, p = 0.031) 
      with the overall ADFI of birds. CONCLUSION: Taken together, the results of this 
      study suggest that the combination of in ovo feeding of GABA and ETM can enhance 
      hepatic antioxidant function in broilers.
FAU - Ncho, Chris Major
AU  - Ncho CM
AD  - Department of Animal Science, Gyeongsang National University, Jinju 52828, Korea.
AD  - Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 
      52828, Korea.
FAU - Goel, Akshat
AU  - Goel A
AD  - Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 
      52828, Korea.
FAU - Gupta, Vaishali
AU  - Gupta V
AD  - Division of Applied Life Sciences (BK21 Plus Program), Gyeongsang National 
      University, Jinju 52828, Korea.
FAU - Jeong, Chae-Mi
AU  - Jeong CM
AD  - Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 
      52828, Korea.
AD  - Division of Applied Life Sciences (BK21 Plus Program), Gyeongsang National 
      University, Jinju 52828, Korea.
FAU - Choi, Yang-Ho
AU  - Choi YH
AD  - Department of Animal Science, Gyeongsang National University, Jinju 52828, Korea.
AD  - Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju 
      52828, Korea.
AD  - Division of Applied Life Sciences (BK21 Plus Program), Gyeongsang National 
      University, Jinju 52828, Korea.
LA  - eng
GR  - Ministry of Science and ICT/
GR  - 2019H1D3A1A01071142/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20220630
PL  - Korea (South)
TA  - Anim Biosci
JT  - Animal bioscience
JID - 101774366
PMC - PMC9834729
OTO - NOTNLM
OT  - Antioxidant Status
OT  - Broiler
OT  - In ovo Feeding
OT  - Thermal Manipulation
OT  - gamma-Aminobutyric acid (GABA)
COIS- CONFLICT OF INTEREST We certify that there is no conflict of interest with any 
      financial organization regarding the material discussed in the manuscript.
EDAT- 2022/07/08 06:00
MHDA- 2022/07/08 06:01
PMCR- 2023/02/01
CRDT- 2022/07/07 19:16
PHST- 2022/03/11 00:00 [received]
PHST- 2022/06/07 00:00 [accepted]
PHST- 2022/07/08 06:00 [pubmed]
PHST- 2022/07/08 06:01 [medline]
PHST- 2022/07/07 19:16 [entrez]
PHST- 2023/02/01 00:00 [pmc-release]
AID - ab.22.0099 [pii]
AID - ab-22-0099 [pii]
AID - 10.5713/ab.22.0099 [doi]
PST - ppublish
SO  - Anim Biosci. 2023 Feb;36(2):284-294. doi: 10.5713/ab.22.0099. Epub 2022 Jun 30.