PMID- 35799735
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1682-024X (Print)
IS  - 1681-715X (Electronic)
IS  - 1681-715X (Linking)
VI  - 38
IP  - 5
DP  - 2022 May-Jun
TI  - Expressions of HIF-1alpha and MiR-210 in aqueous humor of patients with central 
      retinal vein occlusion combined with macular edema.
PG  - 1327-1332
LID - 10.12669/pjms.38.5.5092 [doi]
AB  - OBJECTIVES: To detect the expressions of hypoxia-inducible factor 1alpha (HIF-1alpha) and 
      microRNA-210 (miR-210) in the aqueous humor of patients with central retinal vein 
      occlusion (CRVO) combined with macular edema, and explore their correlations with 
      CRVO combined with macular edema. METHODS: A total of 65 patients (65 eyes) with 
      CRVO combined with macular edema who were treated in the Lixiang Eye Hospital of 
      Soochow University from April 2018 to March 2020 were selected as subjects (CRVO 
      combined with macular edema group). Additionally, 74 patients (74 eyes) with 
      cataract were selected during the same period as cataract control group. The 
      expressions of HIF-1alpha mRNA and miR-210 in the aqueous humor were detected by 
      quantitative real-time PCR (qRT-PCR). The levels of monocyte chemoattractant 
      protein-1 (MCP-1), interleukin-6 (IL-6) and vascular endothelial growth factor 
      (VEGF) in the aqueous humor were measured using enzyme-linked immunosorbent assay 
      (ELISA). The correlations of HIF-1alpha mRNA and miR-210 in the aqueous humor of 
      patients with CRVO combined with macular edema with vasoactive molecule levels, 
      the diagnostic value of HIF-1alpha mRNA and miR-210 levels in the aqueous humor in 
      CRVO combined with macular edema, as well as the factors influencing the 
      occurrence of CRVO combined with macular edema were analyzed. RESULTS: The levels 
      of HIF-1alpha mRNA, miR-210, MCP-1, VEGF and IL-6 in the aqueous humor of the CRVO 
      combined with macular edema group were higher than those of the cataract control 
      group (P < 0.05). In the CRVO combined with macular edema group, HIF-1alpha mRNA and 
      miR-210 levels in the aqueous humor were positively correlated (r = 0.522, P < 
      0.05), and they were positively correlated with MCP-1, VEGF and IL-6 levels (P < 
      0.05). The area under the curve (AUC) of HIF-1alpha mRNA and miR-210 in the aqueous 
      humor in diagnosing CRVO combined with macular edema was 0.888 and 0.866, the 
      specificity was 95.9% and 85.1%, and the sensitivity was 76.9% and 80.0%, 
      respectively. The AUC of their combination was 0.937, with the specificity of 
      93.2% and the sensitivity of 86.2%. HIF-1alpha, miR-210 and VEGF were the independent 
      risk factors affecting the occurrence of CRVO combined with macular edema (P < 
      0.05). CONCLUSION: In patients with CRVO combined with macular edema, HIF-1alpha mRNA 
      and miR-210 were highly expressed in the aqueous humor, which may play an 
      important role in the occurrence and development of the disease.
CI  - Copyright: (c) Pakistan Journal of Medical Sciences.
FAU - Hu, Pinghui
AU  - Hu P
AD  - Pinghui Hu, Department of Ophthalmology, Nanjing University of Traditional 
      Chinese Medicine, Li Xiang Eye Hospital of Soochow University, Suzhou 215000, 
      Jiangsu, China.
FAU - Liu, Guanghui
AU  - Liu G
AD  - Guanghui Liu, Department of Ophthalmology, People's Hospital Affiliated to Fujian 
      University of Traditional Chinese Medicine, Fuzhou 350004, Fujian, China.
FAU - Sun, Huaping
AU  - Sun H
AD  - Huaping Sun, Department of Ophthalmology, Nanjing University of Traditional 
      Chinese Medicine, Jiangsu Provincial Hospital of Traditional Chinese Medicine, 
      Nanjing 210029, Jiangsu, China.
FAU - Wei, Wei
AU  - Wei W
AD  - Wei Wei, Department of Ophthalmology, Nanjing University of Traditional Chinese 
      Medicine, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing 
      210029, Jiangsu, China.
LA  - eng
PT  - Journal Article
PL  - Pakistan
TA  - Pak J Med Sci
JT  - Pakistan journal of medical sciences
JID - 100913117
PMC - PMC9247752
OTO - NOTNLM
OT  - Aqueous humor
OT  - Central retinal vein occlusion
OT  - Hypoxia-inducible factor 1alpha
OT  - Macular edema
OT  - microRNA-210
COIS- Conflicts of interest: None.
EDAT- 2022/07/09 06:00
MHDA- 2022/07/09 06:01
PMCR- 2022/05/01
CRDT- 2022/07/08 02:36
PHST- 2021/07/29 00:00 [received]
PHST- 2022/01/24 00:00 [revised]
PHST- 2022/02/05 00:00 [accepted]
PHST- 2022/07/08 02:36 [entrez]
PHST- 2022/07/09 06:00 [pubmed]
PHST- 2022/07/09 06:01 [medline]
PHST- 2022/05/01 00:00 [pmc-release]
AID - PJMS-38-1327 [pii]
AID - 10.12669/pjms.38.5.5092 [doi]
PST - ppublish
SO  - Pak J Med Sci. 2022 May-Jun;38(5):1327-1332. doi: 10.12669/pjms.38.5.5092.