PMID- 35801397
OWN - NLM
STAT- MEDLINE
DCOM- 20220719
LR  - 20220818
IS  - 2576-6422 (Electronic)
IS  - 2576-6422 (Linking)
VI  - 5
IP  - 7
DP  - 2022 Jul 18
TI  - Gas Cluster Ion Beams as a Versatile Soft-Landing Tool for the Controlled 
      Construction of Thin (Bio)Films.
PG  - 3180-3192
LID - 10.1021/acsabm.2c00169 [doi]
AB  - Surface biofunctionalization with proteins is the key to many biomedical 
      applications. In this study, a solvent-free method for the controlled 
      construction of protein thin films is reported. Using large argon gas cluster ion 
      beams, proteins are sputtered from a target (a pool of pure proteins), and 
      collected on a chosen substrate, being nearly any solid material. Time-of-flight 
      secondary ion mass spectrometry (ToF-SIMS) revealed the presence of intact 
      protein molecules on the collectors. Furthermore, lowering the energy per atom in 
      the cluster projectiles down to 1 eV/atom allowed more than 60% of bradykinin 
      molecules to be transferred intact. This protein deposition method offers a 
      precise control of the film thickness as the transferred protein quantity is 
      proportional to the argon clusters ion dose reached for the transfer. This major 
      feature enables building protein films from (sub)mono- to multilayers, without 
      upper limitation of the thickness. A procedure was developed to measure the film 
      thickness in situ the ToF-SIMS instrument. The versatility and potential of this 
      soft-landing alternative for further applications is demonstrated on the one hand 
      by building a protein thin film at the surface of paper, a substrate hardly 
      compatible with solution-based adsorption methods. On the other hand, the 
      possibility to achieve alternated multilayer buildup is demonstrated with the 
      construction of a bilayer composed of bradykinin and Irganox, with the two layers 
      well separated. These results lay the first stone toward original and complex 
      multilayers that could previously not be considered with solution-based 
      adsorption methods, and this regardless of the substrate nature.
FAU - Delmez, Vincent
AU  - Delmez V
AUID- ORCID: 0000-0002-9203-9919
AD  - Institute of Condensed Matter and Nanosciences - Bio & Soft Matter, Universite 
      catholique de Louvain, Place Louis Pasteur, 1 bte L4.01.10, B-1348 
      Louvain-la-Neuve, Belgium.
FAU - Tomasetti, Benjamin
AU  - Tomasetti B
AD  - Institute of Condensed Matter and Nanosciences - Bio & Soft Matter, Universite 
      catholique de Louvain, Place Louis Pasteur, 1 bte L4.01.10, B-1348 
      Louvain-la-Neuve, Belgium.
FAU - Daphnis, Thomas
AU  - Daphnis T
AD  - Institute of Condensed Matter and Nanosciences - Bio & Soft Matter, Universite 
      catholique de Louvain, Place Louis Pasteur, 1 bte L4.01.10, B-1348 
      Louvain-la-Neuve, Belgium.
FAU - Poleunis, Claude
AU  - Poleunis C
AD  - Institute of Condensed Matter and Nanosciences - Bio & Soft Matter, Universite 
      catholique de Louvain, Place Louis Pasteur, 1 bte L4.01.10, B-1348 
      Louvain-la-Neuve, Belgium.
FAU - Lauzin, Clement
AU  - Lauzin C
AUID- ORCID: 0000-0001-5044-5137
AD  - Institute of Condensed Matter and Nanosciences - Bio & Soft Matter, Universite 
      catholique de Louvain, Place Louis Pasteur, 1 bte L4.01.10, B-1348 
      Louvain-la-Neuve, Belgium.
FAU - Dupont-Gillain, Christine
AU  - Dupont-Gillain C
AUID- ORCID: 0000-0002-6153-4044
AD  - Institute of Condensed Matter and Nanosciences - Bio & Soft Matter, Universite 
      catholique de Louvain, Place Louis Pasteur, 1 bte L4.01.10, B-1348 
      Louvain-la-Neuve, Belgium.
FAU - Delcorte, Arnaud
AU  - Delcorte A
AUID- ORCID: 0000-0003-4127-8650
AD  - Institute of Condensed Matter and Nanosciences - Bio & Soft Matter, Universite 
      catholique de Louvain, Place Louis Pasteur, 1 bte L4.01.10, B-1348 
      Louvain-la-Neuve, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220708
PL  - United States
TA  - ACS Appl Bio Mater
JT  - ACS applied bio materials
JID - 101729147
RN  - 67XQY1V3KH (Argon)
RN  - S8TIM42R2W (Bradykinin)
SB  - IM
MH  - Argon/chemistry
MH  - *Bradykinin
MH  - *Spectrometry, Mass, Secondary Ion/methods
OTO - NOTNLM
OT  - (bio)functionalization
OT  - ToF-SIMS
OT  - gas cluster ion beam (GCIB)
OT  - multilayers
OT  - proteins
OT  - solvent-free deposition
OT  - thin films
EDAT- 2022/07/09 06:00
MHDA- 2022/07/20 06:00
CRDT- 2022/07/08 04:23
PHST- 2022/07/09 06:00 [pubmed]
PHST- 2022/07/20 06:00 [medline]
PHST- 2022/07/08 04:23 [entrez]
AID - 10.1021/acsabm.2c00169 [doi]
PST - ppublish
SO  - ACS Appl Bio Mater. 2022 Jul 18;5(7):3180-3192. doi: 10.1021/acsabm.2c00169. Epub 
      2022 Jul 8.